thumbnail image
tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Product 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Natural Compounds
    • Synthetic Biology
    • Enzymes
  • POCT Solution 
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Freeze-Drying System
    • Lateral Flow System
  • About 
    • About SBS
    • Achievements
    • Ecosystem
    • Legal Statement
  • Contact
  • …  
    • Home
    • Product 
      • All Products
      • Custom Services
      • Catalog Products
      • Innovative Systems
      • Nucleic Acid Related
      • Natural Compounds
      • Synthetic Biology
      • Enzymes
    • POCT Solution 
      • LAMP
      • RPA
      • CRISPR
      • DNA-Free Enzymes
      • Freeze-Drying System
      • Lateral Flow System
    • About 
      • About SBS
      • Achievements
      • Ecosystem
      • Legal Statement
    • Contact
    • Login
tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Product 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Natural Compounds
    • Synthetic Biology
    • Enzymes
  • POCT Solution 
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Freeze-Drying System
    • Lateral Flow System
  • About 
    • About SBS
    • Achievements
    • Ecosystem
    • Legal Statement
  • Contact
  • …  
    • Home
    • Product 
      • All Products
      • Custom Services
      • Catalog Products
      • Innovative Systems
      • Nucleic Acid Related
      • Natural Compounds
      • Synthetic Biology
      • Enzymes
    • POCT Solution 
      • LAMP
      • RPA
      • CRISPR
      • DNA-Free Enzymes
      • Freeze-Drying System
      • Lateral Flow System
    • About 
      • About SBS
      • Achievements
      • Ecosystem
      • Legal Statement
    • Contact
    • Login
Beijing SBS Genetech Co.,Ltd.
Go Back
SELEX Aptamer Library Screening

SELEX Aptamer Library Screening

SELEX (Systematic Evolution of Ligands by Exponential Enrichment) is a technique used to screen highly specific nucleic acid aptamers from a random single-stranded nucleic acid sequence library. The fundamental concept of SELEX is to chemically synthesize an in vitro single-stranded oligonucleotide library, mix it with the target substance, and through repeated rounds of selection and amplification, wash away nucleic acid molecules that do not bind or have low affinity for the target substance, thereby isolating and purifying nucleic acid aptamers with high affinity for the target substance.
Get a Quote
More Details

Description

SELEX (Systematic Evolution of Ligands by Exponential Enrichment) is a technique used to screen highly specific nucleic acid aptamers from a random single-stranded nucleic acid sequence library. The fundamental concept of SELEX is to chemically synthesize an in vitro single-stranded oligonucleotide library, mix it with the target substance, and through repeated rounds of selection and amplification, wash away nucleic acid molecules that do not bind or have low affinity for the target substance, thereby isolating and purifying nucleic acid aptamers with high affinity for the target substance.

SELEX technology and nucleic acid aptamers offer several advantages, including a large library capacity, broad applicability, high resolution, strong practicality, high affinity, relatively simple, fast, and economical screening processes, and small aptamer size. This technology has applications in in vitro diagnostics, in vivo therapy, and drug development.

Client Provided Samples

Applicable Targets for SELEX Aptamer Screening Services: Proteins, small molecules, cells, bacteria, etc.

Protein Sample Requirements: Please provide the protein size, volume, concentration, buffer composition, protein gel image, and information on whether the protein contains any tags and the corresponding tag details.

  1. Proteins should not contain large tags.
  2. Buffers should not contain free amines, such as sodium azide.
  3. Tris buffer should not be used, and proteins should be stable in PBS buffer.
  4. Purity should be greater than 95% (recommended >95%, minimum 90%).
  5. Total protein amount should be 500 μg to 1 mg, with a concentration of 1 mg/ml.

Small Molecule Sample Requirements: Please provide the sample mass, concentration, volume, and storage conditions.

  1. Good water solubility (≥1 mM).
  2. Stability: resistant to oxidation and lactonization, etc.
  3. Minimal heat of dilution.
  4. Small molecule purity >95% (recommended >95%, minimum 90%).
  5. Sample amount: 10 mg.

Special Note: It is recommended to send small molecules in dry powder form. They can be transported with ice packs, or dry ice for added safety, depending on the storage requirements of the small molecules.

For Cells and Bacterial Targets: Please consult for specific sample requirements and quantities before sending.

 

Delivery

Final Product Delivery:

  • Five aptamer sequences selected by the client

Delivery Report:

  • NGS sequencing results
  • Complete experimental report (including experimental procedures, enrichment rate data during the screening process, related photos, etc.)

 

Related Projects

Project:

  • Aptamer Affinity Characterization Service

Project Description:

  • Affinity characterization service supporting the aptamer screening service, primarily for determining the affinity between the target (protein or small molecule) and the aptamer. Specific charges need to be consulted.
 
 
 
 
With over 20 years of leading experience in oligo synthesis, SBS Genetech is recognized as one of the global major leading industry players in Oligonucleotide Synthesis by third-party market researchers. For more details, please visit Oligonucleotide Synthesis Services Market landscape, Top Competitor Analysis, Revenue, Sales With Forecast Data from 2022 to 2028.
 

Selected Published Papers

SBS Genetech supplies high-quality oligonucleotides, empowering researchers to publish over 800 papers in prestigious journals such as Nucleic Acids Research, Biosensors and Bioelectronics, and Blood

Measuring specific interaction of transcription factor ZmDREB1A with its DNA responsive element at the molecular level

Nucleic Acids Research | 01 Jan 2004 | DOI: https://doi.org/10.1093/nar/gnh100

All the DNA sequences were custom synthesized from SBS Genetech Co. Ltd. (Beijing, China). These include the DRE element sequence (ACCGAC), 5′-NH 2 -GATATACT ACCGAC ATGAGTTC-3′, and its complementary ssDNA, 3′-CTATATGATGGCTGTACTCAAG-5′; the DRE element sequence (GCCGAC), 5′-NH 2 -GATATACT GCCGAC ATGAGTTC-3′, and its complementary ssDNA, 3′-CTATATGACGGCTGTACTCAAG-5′; the ERE element (GCCGCC), 5′-NH 2 -CGCAGACATA GCCGCC ATTT-3′, and its complementary ssDNA, 3′-GCGTCTGTATCGGCGGTAAA-5′; the mutant DRE element sequence (ACCGAG), 5′-NH 2 -GATATACT ACCGAG ATGAGTTC-3′, and its complementary ssDNA, 3′-CTATATGATGGCTCTACTCAAG-5′ (the element sequences are underlined).

 

Novel heat shock protein Hsp70L1 activates dendritic cells and acts as a Th1 polarizing adjuvant

Blood | 01 March 2004 | DOI: https://doi.org/10.1182/blood-2003-08-2828

To assess Hsp70L1 expression in DCs, 6-day DCs generated from human peripheral monocytes were stimulated at 5 × 105 cells/mL for 8 hours with 1 μg/mL lipopolysaccharide (LPS) (Sigma), 30 μg/mL CpG oligonucleotides (5′-TCGTCGTTCCCCCCCCCC-CC-3′; SBS Genetech, Beijing, China)

 

A thermodynamic overview of naturally occurring intramolecular DNA quadruplexes

Nucleic Acids Research | 30 Aug 2008 | DOI: https://doi.org/10.1093/nar/gkn543

HPLC purified oligonucleotides were procured from SBS Genetech, China. The concentrations of these oligonucleotides were calculated by extrapolation of tabulated values of the monomer bases and dimers at 25°C using procedures reported earlier

 

Quartz crystal microbalance detection of protein amplified by nicked circling, rolling circle amplification and biocatalytic precipitation

Biosensors and Bioelectronics | 31 Oct 2014 | DOI: https://doi.org/10.1016/j.bios.2014.10.055

Deoxyribonucleoside 5′-triphosphates (dNTPs) mixture and all oligonucleotides as depicted in Table S1 of Supporting information were purchased from SBS Genetech. Co. Ltd. (China).

 

Electrochemical aptasensor based on one-step synthesis of Cu2O@aptamer nanospheres for sensitive thrombin detection

Sensors and Actuators B: Chemical | 10 June 2015 | DOI: https://doi.org/10.1016/j.snb.2015.05.089

Blood sera and aptamer were acquired from SBS Genetech Co. Ltd. (Beijing, China) and has the following sequences: 5′-TCTCTCAGTCCGTGGTAGGGCAGGGTTGGGGTGACT-3′......

 

Carbon-based nanocomposites with aptamer-templated silver nanoclusters for the highly sensitive and selective detection of platelet-derived growth factor

Biosensors and Bioelectronics | 16 Nov 2016 | DOI: https://doi.org/10.1016/j.bios.2016.11.019

Aptamer was obtained from SBS Genetech Co., Ltd. (Beijing, China), and the sequence was: 5′-CAG GCT ACG GCA CGT AGA GCA TCA CCA TGA TCC

 

Fe(III)-based metal–organic framework-derived core–shell nanostructure: Sensitive electrochemical platform for high trace determination of heavy metal ions

Biosensors and Bioelectronics | 17 Mar 2017 | DOI: https://doi.org/10.1016/j.bios.2017.03.014

Aptamer strands were obtained from SBS Genetech Co. Ltd (Beijing, China).

 

A CRISPR/Cas12a-based fluorescence aptasensor for the rapid and sensitive detection of ampicillin

International Journal of Biological Macromolecules | 9 June 2023 | DOI: https://doi.org/10.1016/j.ijbiomac.2023.125211

The oligonucleotides employed in this experiment were procured from Beijing SBS Genetech Co. Ltd. (China). The sequences of the reporter and three aptamers were obtained in accordance with their relevant references [36,37], while crRNA and activator DNA sequences were designed in our laboratory.

 

Smart co-delivery of plasmid DNA and doxorubicin using MCM-chitosan-PEG polymerization functionalized with MUC-1 aptamer against breast cancer

Biomedicine & Pharmacotherapy | 19 March 2024 | DOI: https://doi.org/10.1016/j.biopha.2024.116465

The aptamer used (sequence: 5′-GCCCGCCGTGGCTGGGTCTTCCTTGGTCGGTCTACAAAAAAAAAA-SH-3′) was obtained from SBS Genetech Co. Ltd.

 

Exploring aptamer-aTF sandwich and CRISPR-Cas12a methods for sensitive L-lactate biosensing in human serum and saliva

Sensors and Actuators B: Chemical | 28 November 2024 | DOI: https://doi.org/10.1016/j.snb.2024.137015

The oligonucleotides utilised in this study were obtained from Beijing SBS Genetech Co. Ltd. (Beijing, China). The sequences of the dsDNA, reporter, and aptamers were obtained following their relevant references while gRNA...

 

Nucleic acid-free aptamer-CRISPR/Cas14 biosensor for prosthetic joint infection rapid detection

Sensors and Actuators Reports | 24 March 2025 | DOI: https://doi.org/10.1016/j.snr.2025.100318

Nucleotide sequences were synthesized by Beijing SBS Genetech Co., Ltd.

 
 
 
 
 
 
Only for research and not intended for treatment of humans or animals
 
 

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

 

 

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

 

  • background image
    background image
    background image
    background image
    background image
    background image
    background image
  • Featured Publications

    We are honored to create value for our customers and facilitate the development of science

SBS Genetech © Copyright 2000-2025

from China, for the World

for Superior Biology Services since 2000

    Home
    Journals
    Contact
    Posts
Cookie Use
We use cookies to ensure a smooth browsing experience. By continuing we assume you accept the use of cookies.
Learn More