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tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Products 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Enzymes
  • POCT 
    • Integrated POCT Platform
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Lateral Flow System
    • Freeze-Drying System
  • About 
    • About SBS
    • Solutions
    • Achievements
    • Legal Statement
  • Contact
  • …  
    • Home
    • Products 
      • All Products
      • Custom Services
      • Catalog Products
      • Innovative Systems
      • Nucleic Acid Related
      • Enzymes
    • POCT 
      • Integrated POCT Platform
      • LAMP
      • RPA
      • CRISPR
      • DNA-Free Enzymes
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      • Freeze-Drying System
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pUBI10-GUS

pUBI10-GUS

pUBI10-GUS (reporter gene plasmid) is a self-developed reporter gene plasmid for transient transfection of plant (such as Arabidopsis, etc.) protoplasts.
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The main information of pUBI10-GUS plasmid is as follows:

  • Feature Nucleotide
  • Position
  • NOS terminator
  • 1-253
  • M13 forward primer(M13 fwd)
  • 262-278
  • f1 origin of ss-DNA replication
  • 491-946
  • Ampicillin promoter
  • 1228-1332
  • Ampicillin resistance ORF
  • 1333-2193
  • pUC origin
  • 2364-2952
  • CAP binding site
  • 3240-3261
  • lac promoter
  • 3276-3306
  • lac operator
  • 3314-3330
  • M13 reverse primer(M13 rev)
  • 3338-3354
  • UBI10 promoter
  • 3388-4704
  • β-glucuronidase(GUS)
  • 4732-6543

The map of pUBI10-GUS plasmid (6614bp) is as follows:

pUBI10-GUS

Restriction enzymes that do not cut pUBI10-GUS include:

Acc65IAfeIAgeIApaIAscIAsiSI
AvrIIBamHIBbvCIBlpIBmgBIBmtI
BseRIBsiWIBspDIBspEIBstAPIBstEII
BstXIBstZ17IBsu36IClaICspCIEagI
EcoNIFseIHpaIKpnINcoINdeI
NheINotIPacIPaeR7IPflMIPmeI
PspOMIPspXIRsrIISacIISexAISfiI
SgrAISpeISphISrfIStuIStyI
SwaIXcmIXhoI


Restriction enzymes that cut pUBI10-GUS once include:

AatIIG,ACGT`C1197NmeAIIIGCCGAG(N)19,NN`1970
AflIIA`CRYG,T30NsiIA,TGCA`T121
AhdIGACNN,N`NNGTC2121PflFIGACN`N,NGTC4267
BaeI,(N)5`(N)1ACNNNNGTAYC(N)7,(N)5`3673PluTIG,GCGC`C417
BbsIGAAGACNN`NNNN,4508PmlICAC|GTG4216
BglIIA`GATC,T4634PpuMIRG`GWC,CY3925
BpmICTGGAG(N)14,NN`2049PshAIGACNN|NNGTC5305
BsaIGGTCTCN`NNNN,2055PstIC,TGCA`G3387
BseYIC`CCAG,C2704SbfICC,TGCA`GG3382
BsgIGTGCAG(N)14,NN`6251ScaIAGT|ACT1640
BsmIGAATG,CN`4787SfoIGGC|GCC419
BstBITT`CG,AA5825SmaICCC|GGG4714
DraIIICAC,NNN`GTG721SnaBITAC|GTA5116
HindIIIA`AGCT,T3368TspMICCCGG,G4711
KasIG`GCGC,C417Tth111IGACN`N,NGTC4267
MfeIC`AATT,G4862XmaIC`CCGG,G4711
MscITGG|CCA5353ZraIGAC|GTC1199
NarIGG`CG,CC418


The sequencing primer sequences that can be used for pUBI10-GUS plasmid are as follows:

GUS promoter primer(4744-4762): 5'-CACGGGTTGGGGTTTCTAC-3'

M13 reverse primer(3338-3354): 5'-CAGGAAACAGCTATGAC-3'


Storage Conditions

Store at -20 °C.


Precautions

  • No commercial use of this plasmid is permitted without written permission from SBS Genetech. Nor shall it be transferred to any individual or institution outside the purchaser’s laboratory.
  • This product is intended solely for scientific‑research use by professionals. It shall not be adopted for clinical diagnosis or therapy, food‑related or pharmaceutical applications, nor kept in residential premises.
  • For your safety and health, wear a lab coat and disposable gloves during operation.

 

 

Only for research and not intended for treatment of humans or animals

 

 

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