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tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Products 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Enzymes
  • POCT 
    • Integrated POCT Platform
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Lateral Flow System
    • Freeze-Drying System
  • About 
    • About SBS
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    • Legal Statement
  • Contact
  • …  
    • Home
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      • All Products
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pTac-His-MBP-WELQ

pTac-His-MBP-WELQ

pTac-His-MBP-WELQ is a prokaryotic expression plasmid used to fuse and express a double tag (His tag) and an MBP tag (MBP tag) at the N-terminus of the target protein at the same time, and there is a WELQ Protease (P2311) restriction site between the two tags and the target protein. The fusion expression of the MBP tag is conducive to improving the expression level and protein stability of the target protein, and promoting the solubility and correct folding of the protein; while the His tag is conducive to the separation and purification of the target protein MBP fusion protein through a nickel column. Using this plasmid, the target protein containing His and MBP double tags obtained after nickel column purification can be digested by WELQ Protease to remove the His tag and MBP tag.
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All products have special prices for bulk purchase, please contact for more details if required.

 

 

The main information of pTac-His-MBP-WELQ plasmid is as follows:
 

  • Feature Nucleotide
  • Position
  • lacI promoter
  • 3-80
  • LacI coding sequence
  • 81-1163
  • Tac promoter
  • 1406-1434
  • lac operator
  • 1442-1458
  • His tag coding sequence
  • 1531-1560
  • MBP tag coding sequence
  • 1561-2658
  • WELQ site
  • 2683-2694
  • Multiple cloning sites (PstⅠ-XhoI)
  • 2689-2750
  • M13 forward primer (M13 fwd)
  • 2754-2770
  • Ampicillin resistance ORF
  • 3435-4295
  • f1 origin
  • 4337-4850
  • pBR322 origin
  • 4961-5549

 

The map of pTac-His-MBP-WELQ plasmid (6663bp) is as follows:

pTac-His-MBP-WELQ

Restriction sites not found in pTac-His-MBP-WELQ include:
 

AarIAatIIAbsIAflIIAgeIAleIAscI
AsiSIAvrIIBaeIBarIBbvCIBmtIBplI
BseRIBspDIBsrGIBstBIBsu36IClaICspCI
EagIEcoNIFalIFseII-CeuII-PpoII-SceI
KflIMauBIMfeIMreINheINotINruI
PacIPasIPI-PspIPI-SceIPmeIPmlIPshAI
PsrIRsrIISbfISexAISfiISgrAISgrDI
SnaBISpeISphISrfIStuIXmnIZraI
 

The single restriction sites in pTac-His-MBP-WELQ include:
 

Acc65IG`GTAC,C2733NcoIC`CATG,G2739
AhdIGACNN,N`NNGTC4222NdeICA`TA,TG1526
ApaIG,GGCC`C642NgoMIVG`CCGG,C4720
BamHIG`GATC,C2727NsiIA`TGCA,T1532
BglIIA`GATC,T1917PaeR7IC`TCGA,G2745
BlpIGC`TNA,GC2344PciIA`CATG,T5605
BmgBICAC|GTC2100PflFIGACN`N,NGTC5863
Bpu10ICC`TNA,GC6500PflMICCAN,NNN`NTGG13
BsiWIC`GTAC,G1848PluTIG,GCGC`C1074
BsmIGAATG,CN`2178PspOMIG`GGCC,C638
BspQIGCTCTTCN`NNN,5722PspXIVC`TCGA,GB2745
BstEIIG`GTNAC,C612PstIC,TGCA`G2693
BstZ17IGTA|TAC5838SacIG,AGCT`C2668
DraIIICAC,NNN`GTG4619SacIICC,GC`GG2506
Eco53kIGAG|CTC2666SalIG`TCGA,C2707
EcoRIG`AATT,C2695SapIGCTCTTCN`NNN,5722
EcoRVGAT|ATC881ScaIAGT|ACT3741
FspAIRTGC|GCAY6628SfoIGGC|GCC1072
HindIIIA`AGCT,T2701SmaICCC|GGG2723
HpaIGTT|AAC937StyIC`CWWG,G2739
KasIG`GCGC,C1070SwaIATTT|AAAT4394
KpnIG,GTAC`C2737TspMIC`CCGG,G2721
MluIA`CGCG,T431Tth111IGACN`N,NGTC5863
MscITGG|CCA6638XbaIT`CTAG,A2713
NaeIGCC|GGC4722XhoIC`TCGA,G2745
NarIGG`CG,CC1071XmaIC`CCGG,G2721
  
 

The recommended sequencing primer sequences for pTac-His-MBP-WELQ plasmid are as follows:
Forward primer (2754-2770): 5'-ACTGGCCGTCGTTTTAC-3'
Reverse primer (1446-1469): 5'-GAGCGGATAACAATTTCACACAGG-3'


Storage Conditions

Store at -20 °C.


Precautions

  • No commercial use of this plasmid is permitted without written permission from SBS Genetech. Nor shall it be transferred to any individual or institution outside the purchaser’s laboratory.
  • This product is intended solely for scientific‑research use by professionals. It shall not be adopted for clinical diagnosis or therapy, food‑related or pharmaceutical applications, nor kept in residential premises.
  • For your safety and health, wear a lab coat and disposable gloves during operation.

 

 

Only for research and not intended for treatment of humans or animals

 

 

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