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tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Products 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Enzymes
  • POCT 
    • Integrated POCT Platform
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Lateral Flow System
    • Freeze-Drying System
  • About 
    • About SBS
    • Solutions
    • Achievements
    • Legal Statement
  • Contact
  • …  
    • Home
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      • All Products
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pTac-His-MBP-PreScission

pTac-His-MBP-PreScission

pTac-His-MBP-PreScission is a prokaryotic expression plasmid that is used to fuse and express a double tag (His tag) and an MBP tag (MBP tag) at the N-terminus of the target protein at the same time, and has a PreScission restriction site before the tag and the target protein. The fusion expression of the MBP tag is conducive to improving the expression level and protein stability of the target protein, and promoting the solubility and correct folding of the protein; while the His tag is conducive to the separation and purification of the target protein MBP fusion protein through a nickel column. Using this plasmid, the target protein containing His and MBP double tags obtained after nickel column purification can be digested with PreScission Protease (P2302/P2303) to remove the His tag and MBP tag.
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More Details

All products have special prices for bulk purchase, please contact for more details if required.

 

 

The main information of pTac-His-MBP-PreScission plasmid is as follows:

 

  • Feature Nucleotide
  • Position
  • LacI promoter
  • 3-80
  • LacI coding sequence
  • 81-1163
  • Tac promoter
  • 1406-1434
  • Lac operator
  • 1442-1458
  • His-tag coding sequence
  • 1531-1560
  • MBP-tag coding sequence
  • 1561-2658
  • PreScission Protease recognition site sequence
  • 2671-2694
  • Multiple cloning sites (EcoRI -XhoI)
  • 2696-2752
  • M13 forward primer (M13 fwd)
  • 2760-2776
  • Ampicillin resistance ORF
  • 3441-4301
  • f1 origin
  • 4401-4856
  • pBR322 origin
  • 4967-5555

 

The map of pTac-His-MBP-PreScission plasmid (6669bp) is as follows:

pTac-His-MBP-PreScission

 

Restriction enzymes that do not cut pTac-His-MBP-PreScission include:
 

AatII    AflII    AgeI    AleI    AscI    AsiSI    AvrII
BaeI    BbvCI    BmtI    BseRI    BspDI    BsrGI    BstBI
Bsu36I    ClaI    CspCI    EagI    EcoNI    FseI    MfeI
NheI    NotI    NruI    PacI    PaqCI    PmeI    PmlI
PshAI    RsrII    SbfI    SexAI    SfiI    SgrAI    SnaBI
SpeI    SphI    SrfI    StuI    XmnI    ZraI    
 

The single restriction enzyme cutting sites (Restriction enzymes that cut pTac-His-MBP-PreScission) in pTac-His-MBP-PreScission include:
 

Acc65IG`GTAC,C2739NdeICA`TA,TG1526
AhdIGACNN,N`NNGTC4227NgoMIVG`CCGG,C4726
BamHIG`GATC,C2733NsiIA`TGCA,T1528
BglIIA`GATC,T1917PaeR7IC`TCGA,G2751
BlpIGC`TNA,GC2344PciIA`CATG,T5611
BmgBICAC|GTC2100PflFIGACN`N,NGTC5869
Bpu10ICC`TNA,GC6506PflMICCAN,NNN`NTGG10
BsiWIC`GTAC,G1848PluTIG,GCGC`C1070
BsmIGAATG,CN`2176PspXIVC`TCGA,GB2751
BspQIGCTCTTCN`NNN,5728PstIC,TGCA`G2719
BstEIIG`GTNAC,C612SacIG,AGCT`C2664
BstZ17IGTA|TAC5844SacIICC,GC`GG2504
DraIIICAC,NNN`GTG4622SalIG`TCGA,C2707
Eco53kIGAG|CTC2666SapIGCTCTTCN`NNN,5728
EcoRIG`AATT,C2695ScaIAGT|ACT3747
EcoRVGAT|ATC881SfoIGGC|GCC1072
HindIIIA`AGCT,T2701SmaICCC|GGG2729
HpaIGTT|AAC937StyIC`CWWG,G2745
KasIG`GCGC,C1070SwaIATTT|AAAT4400
KpnIG,GTAC`C2739TspMIC`CCGG,G2727
MluIA`CGCG,T431Tth111IGACN`N,NGTC5869
MscITGG|CCA6644XbaIT`CTAG,A2713
NaeIGCC|GGC4728XhoIC`TCGA,G2751
NarIGG`CG,CC1071XmaIC`CCGG,G2727
NcoIC`CATG,G2745 


Storage Conditions

Store at -20 °C.


Precautions

  • No commercial use of this plasmid is permitted without written permission from SBS Genetech. Nor shall it be transferred to any individual or institution outside the purchaser’s laboratory.
  • This product is intended solely for scientific‑research use by professionals. It shall not be adopted for clinical diagnosis or therapy, food‑related or pharmaceutical applications, nor kept in residential premises.
  • For your safety and health, wear a lab coat and disposable gloves during operation.

 

 

Only for research and not intended for treatment of humans or animals

 

 

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