thumbnail image
tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Products 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Enzymes
  • POCT 
    • Integrated POCT Platform
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Lateral Flow System
    • Freeze-Drying System
  • About 
    • About SBS
    • Solutions
    • Achievements
    • Legal Statement
  • Contact
  • …  
    • Home
    • Products 
      • All Products
      • Custom Services
      • Catalog Products
      • Innovative Systems
      • Nucleic Acid Related
      • Enzymes
    • POCT 
      • Integrated POCT Platform
      • LAMP
      • RPA
      • CRISPR
      • DNA-Free Enzymes
      • Lateral Flow System
      • Freeze-Drying System
    • About 
      • About SBS
      • Solutions
      • Achievements
      • Legal Statement
    • Contact
    • Login
tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Products 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Enzymes
  • POCT 
    • Integrated POCT Platform
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Lateral Flow System
    • Freeze-Drying System
  • About 
    • About SBS
    • Solutions
    • Achievements
    • Legal Statement
  • Contact
  • …  
    • Home
    • Products 
      • All Products
      • Custom Services
      • Catalog Products
      • Innovative Systems
      • Nucleic Acid Related
      • Enzymes
    • POCT 
      • Integrated POCT Platform
      • LAMP
      • RPA
      • CRISPR
      • DNA-Free Enzymes
      • Lateral Flow System
      • Freeze-Drying System
    • About 
      • About SBS
      • Solutions
      • Achievements
      • Legal Statement
    • Contact
    • Login
Beijing SBS Genetech Co.,Ltd.
Go Back
pTac-GST-WELQ

pTac-GST-WELQ

pTac-GST-WELQ is a prokaryotic expression plasmid used to express fusion proteins containing a GST tag (Glutathione S-transferase tag, GST tag) at the N terminus. The N-terminal GST tag of this plasmid contains a WELQ Protease (P2311) enzyme cleavage site, and the N-terminal GST tag can be removed by WELQ Protease.
Get a Quote
More Details

All products have special prices for bulk purchase, please contact for more details if required.

 

 

The main information of pTac-GST-WELQ plasmid is as follows:
 

  • Feature Nucleotide
  • Position
  • tac promoter
  • 183-211
  • lac operator
  • 219-235
  • GST tag coding sequence
  • 258-911
  • WELQ site
  • 930-941
  • Multiple cloning site (PstI-NotI)
  • 936-980
  • AmpR promoter
  • 1286-1390
  • Ampicillin resistance ORF
  • 1391-2251
  • pBR322 origin
  • 2422-3010
  • lacI promoter
  • 3254-3331
  • lacI coding sequence
  • 3332-4414
  • lac promoter
  • 4463-4493
  • lac operator
  • 4501-4517
  • M13 reverse primer (M13 rev)
  • 4525-4541
  • lacZα
  • 4537-81
  • M13 forward primer (M13 fwd)
  • 4557-4573


The map of pTac-GST-WELQ plasmid (4983bp) is as follows:

pTac-GST-WELQ

 

Restriction sites not found in pTac-GST-WELQ include:
 

AarIAbsIAccIAfeIAflIIAgeIAjuI
AlfIAloIAscIAsiSIAvrIIBarIBbvCI
BglIIBlpIBmgBIBplIBpu10IBsaBIBseRI
BsiWIBsmIBspDIBspEIBsrGIBstZ17IClaI
CspCIDraIIIEco53kIFalIFseIFspAIHindIII
I-CeuII-PpoII-SceIKasIKflIMauBIMfeI
MreINaeINarINdeINgoMIVNruINsiI
PacIPasIPI-PspIPI-SceIPluTIPmeIPmlI
PpuMIPshAIPsiIPsrIRsrIISacISacII
SalISbfISexAISfiISfoISgrAISgrDI
SmaISnaBISpeISphISrfIStuITspMI
XbaIXmaI    
  

The single restriction sites in pTac-GST-WELQ include:
 

AatIIG,ACGT`C1259EcoNICCTNN`N,NNAGG268
Acc65IG`GTAC,C963EcoRIG`AATT,C954
AhdIGACNN,N`NNGTC2178EcoRVGAT|ATC4132
AleICACNN|NNGTG4843HpaIGTT|AAC4188
AlwNICAG,NNN`CTG2657KpnIG,GTAC`C967
ApaIG,GGCC`C3893MluIA`CGCG,T3682
AvaIC`YCGR,G968MscITGG|CCA465
BamHIG`GATC,C945NcoIC`CATG,G959
BanIIG,RGCY`C3893NhelG`CTAG,C4318
BfuAIACCTGC(N)4`(N)4,62NotIGC`GGCC,GC974
BmeT110ICY`CG,RG969PaeR7IC`TCGA,G968
BmtIG,CTAG`C4322PflFIGACN`N,NGTC1153
BsaIGGTCTCN`(N)4,2112PflMICCAN,NNN`NTGG3264
BsaAIYAC|GTR1160PfoIT`CCNGG,A1050
BsoBIC`YCGR,G968PspOMIG`GGCC,C3889
BspMIACCTGC(N)4`(N)4,62PspXIVC`TCGA,GB968
BssHIIG`CGCG,C4093PstIC,TGCA`G940
BstAPIGCAN,NNN`NTGC3365StyIC`CWWG,G959
BstBITT`CG,AA655SwaIATTT|AAAT685
BstEIIG`GTNAC,C3863Tth111IGACN`N,NGTC1153
Bsu36ICC`TNA,GG4775XhoIC`TCGA,G968
EagIC`GGCC,G974ZraIGAC|GTC1257


The recommended sequencing primer sequences for pTac-GST-WELQ plasmid are as follows:
Forward Primer (869-891): 5'-GGGCTGGCAAGCCACGTTTGGTG-3'

Reverse Primer (1033-1055): 5'-CCGGGAGCTGCATGTGTCAGAGG-3'


Storage Conditions

Store at -20 °C.


Precautions

  • No commercial use of this plasmid is permitted without written permission from SBS Genetech. Nor shall it be transferred to any individual or institution outside the purchaser’s laboratory.
  • This product is intended solely for scientific‑research use by professionals. It shall not be adopted for clinical diagnosis or therapy, food‑related or pharmaceutical applications, nor kept in residential premises.
  • For your safety and health, wear a lab coat and disposable gloves during operation.

 

 

Only for research and not intended for treatment of humans or animals

 

 

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

 

 

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

 

  • Precision Molecular Technologies

    from Research to Commercial Scale

    For over 25 years, SBS Genetech has delivered

    high-quality, rigorously validated molecular reagents

    trusted by leading research institutions and

    biotechnology companies across 60+ countries.

    Explore Products
    OEM & Custom Solutions
  • Scientific Validation Behind Our Technologies

    We are honored to create value for our customers and facilitate the development of science

SBS Genetech © Copyright 2000-2026

from China, for the World

for Superior Biology Services since 2000

    Home
    Journals
    Contact
    Posts
Cookie Use
We use cookies to ensure a smooth browsing experience. By continuing we assume you accept the use of cookies.
Learn More