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tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Products 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Enzymes
  • POCT 
    • Integrated POCT Platform
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Lateral Flow System
    • Freeze-Drying System
  • About 
    • About SBS
    • Solutions
    • Achievements
    • Legal Statement
  • Contact
  • …  
    • Home
    • Products 
      • All Products
      • Custom Services
      • Catalog Products
      • Innovative Systems
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    • POCT 
      • Integrated POCT Platform
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pT7-N-His-WELQ

pT7-N-His-WELQ

pT7-N-His-WELQ is a prokaryotic expression plasmid used to express fusion proteins containing His tag at the N terminus. The N-terminal His tag of this plasmid contains a WELQ Protease (P2311) restriction site, and the N-terminal His tag can be removed by WELQ Protease.
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The main information of pT7-N-His-WELQ plasmid is as follows:
 

  • Feature Nucleotide
  • Position
  • f1 origin
  • 12-467
  • Kanamycin resistance ORF
  • 560-1375
  • pBR322 origin
  • 1497-2085
  • lacI coding sequence
  • 3515-4597
  • lacI promoter
  • 4598-4675
  • T7 promoter
  • 4988-5006
  • lac operator
  • 5007-5031
  • His tag coding sequence
  • 5079-5096
  • WELQ site
  • 5109-5120
  • Multiple cloning site (PstI-XhoI)
  • 5115-5162
  • T7 Terminator
  • 5229-5276

 

The map of pT7-N-His-WELQ plasmid (5301bp) is as follows:

 

pT7-N-His-WELQ

 

Restriction sites not found in pT7-N-His-WELQ include:
 

AarIAatIIAbsIAcc65IAflIIAgeIAhdI
AleIAscIAvrIIBaeIBbvCIBfuAIBmgBI
BmtIBplIBsaIBseRIBsiWIBspMIBsrGI
BstBIBsu36ICspCIDraIEagIEco53kIFalI
FseII-CeuII-PpoII-SceIKflIKpnIMauBI
MfeIMreIMscINcoINheINotIPacI
PasIPI-PspIPI-SceIPmeIPmlIPspXIPsrI
RsrIISacISacIISbfIScaISexAISfiI
SgrDISnaBISpeISrfIStuISwaIZraI
 

The single restriction sites in pT7-N-His-WELQ include:
 

AcuICTGAAG(N)14,NN`1599NmeAIIIGCCGAG(N)18-19,NN`4042
AlwNICAG,NNN`CTG1732NruITCG|CGA1286
ApaIG,GGCC`C4039PaeR7IC`TCGA,G5157
AsiSIGCG,AT`CGC945PciIA`CATG,T2141
BamHIG`GATC,C5121PflFIGACN`N,NGTC2399
BclIT`GATC,A4228PpuMIRG`GWC,CY3136
BglIGCCN,NNN`NGGC3185PshAIGACNN|NNGTC3401
BglIIA`GATC,T5151PsiITTA|TAA370
BlpIGC`TNA,GC5218PspOMIG`GGCC,C4035
BspQIGCTCTTCN`NNN,2258PstIC,TGCA`G5119
BssHIIG`CGCG,C3831PvuICG,AT`CG945
BssSaIC`ACGA,G1968SalIG`TCGA,C5145
BstAPIGCAN,NNN`NTGC4566SapIGCTCTTCN`NNN,2258
BstEIIG`GTNAC,C4060SgrAICR`CCGG,YG4923
BstZ17IGTA|TAC2374SmaICCC|GGG1069
DraIIICAC,NNN`GTG245SphIG,CATG`C4775
EcoRIG`AATT,C5133StyIC`CWWG,G5240
EcoRVGAT|ATC5141TatIW`GTAC,W2337
FspITGC|GCA3164TspMIC`CCGG,G1067
FspAIRTGC|GCAY3164Tth111IGACN`N,NGTC2399
HindIIIA`AGCT,T5127XbaIT`CTAG,A5034
HpaIGTT|AAC3740XhoIC`TCGA,G5157
MluIA`CGCG,T4242XmaIC`CCGG,G1067
NdeICA`TA,TG5074 
  
 

The recommended sequencing primer sequences for pT7-N-His-WELQ plasmid are as follows:
Forward primer (4988-5006): 5'-TAATACGACTCACTATAGG-3'

Reverse primer (5215-5234): 5'-TGCTAGTTATTGCTCAGCGG-3'


Storage Conditions

Store at -20 °C.


Precautions

  • No commercial use of this plasmid is permitted without written permission from SBS Genetech. Nor shall it be transferred to any individual or institution outside the purchaser’s laboratory.
  • This product is intended solely for scientific‑research use by professionals. It shall not be adopted for clinical diagnosis or therapy, food‑related or pharmaceutical applications, nor kept in residential premises.
  • For your safety and health, wear a lab coat and disposable gloves during operation.

 

 

Only for research and not intended for treatment of humans or animals

 

 

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