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tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Products 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Enzymes
  • POCT 
    • Integrated POCT Platform
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Lateral Flow System
    • Freeze-Drying System
  • About 
    • About SBS
    • Solutions
    • Achievements
    • Legal Statement
  • Contact
  • …  
    • Home
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      • All Products
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      • Catalog Products
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pT7-N-His-SUMO-WELQ

pT7-N-His-SUMO-WELQ

pT7-N-His-SUMO-WELQ is a prokaryotic expression plasmid used to express the target protein containing both His tag and SUMO tag at the N terminus. The His tag is beneficial to the purification of the target protein through affinity chromatography, while the SUMO tag is beneficial to improving the folding of the target protein and increasing the solubility and yield of the target protein. The expressed fusion protein containing the target protein can be digested by PreScission Protease (P2302/P2303) to remove the His tag but retain the SUMO tag, or it can be digested by WELQ Protease (P2311) to remove both His and SUMO tags at the N terminus.
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All products have special prices for bulk purchase, please contact for more details if required.

 

 

The main information of pT7-N-His-SUMO-WELQ plasmid is as follows:
 

  • Feature Nucleotide
  • Position
  • f1 origin
  • 12-467
  • Kanamycin resistance ORF
  • 560-1375
  • PBR322 origin
  • 1497-2085
  • LacI coding sequence
  • 3515-4597
  • LacI promoter
  • 4598-4675
  • T7 promoter
  • 4988-5004
  • lac operator
  • 5007-5031
  • RBS
  • 5046-5068
  • His tag coding sequence
  • 5088-5105
  • PreScission recognition site sequence  
  • 5115-5138
  • SUMO tag coding sequence
  • 5151-5444
  • WELQ site
  • 5454-5465
  • Multiple cloning site (PstI-XhoI)
  • 5460-5498
  • T7 Terminator
  • 5565-5612

 

The map of pT7-N-His-SUMO-WELQ plasmid (5637bp) is as follows:

pT7-N-His-SUMO-WELQ

 

Restriction sites not found in pT7-N-His-SUMO-WELQ include:
 

AarIAatIIAbsIAcc65IAgeIAhdIAscI
AvrIIBaeIBbvCIBfuAIBmgBIBplIBsaI
BseRIBsiWIBspMIBsrGIBstBIBsu36ICspCI
EcoRVFalIFseII-CeuII-PpoII-SceIKflI
KpnIMauBIMfeIMreIMscINcoIPacI
PasIPI-PspIPI-SceIPmeIPmlIPsrIRsrII
SacIISalISbfIScaISexAISfiISgrDI
SnaBISpeISrfIStuISwaIZraI
  

The single restriction sites in pT7-N-His-SUMO-WELQ include:
 

AccIGT`MK,AC2373NheIG`CTAG,C5145
AflIIC`TTAA,G5336NmeAIIIGCCGAG(N)19,NN`4042
AleICACNN|NNGTG5495NotIGC`GGCC,GC5485
AlwNIGAG,NNN`CTG1732NruITCG|CGA1286
AsiSIGCG,AT`CGC945PaeR7IC`TCGA,G5493
BamHIG`GATC,C5442PciIA`CATG,T2141
Bg1IGCCN,NNN`NGGC3182PflFIGACN`N,NGTC2399
Bg1IIA`GATC,T5251PpuMIRG`GWC,CY3136
BmtIG,CTAG`C5149PshAIGACNN|NNGTC3401
BspQIGCTCTTCN`NNN,2258PsiITTA|TAA370
BssHIIG`CGCG,C3831PspXIVC`TCGA,GB5493
BssSaIC`ACGA,G1968PstIC,TGCA`G5464
BstAPIGCAN,NNN`NTGC4566PvuICG,AT`CG945
BstEIIG`GTNAC,C4060SacIG,AGCT`C5476
BstZ17IGTA|TAC2374SapIGCTCTTCN`NNN,2258
DraITTT|AAA5227SgrAICR`CCGG,YG4923
DraIIICAC,NNN`GTG245SmaICCC|GGG1069
EagIC`GGCC,G5485SphIG,CATG`C4775
Eco53kIGAG|CTC5474StyIC`CWWG,G5576
EcoRIG`AATT,C5466TatIW`GTAC,W2337
FspITGC|GCA3164TspMIC`CCGG,G1067
FspAIRTGC|GCAY3164Tth111IGACN`N,NGTC2399
HincIIGTY|RAC3740XbaIT`CTAG,A5034
HindIIIA`AGCT,T5478XhoIC`TCGA,G5493
HpaIGTT|AAC3740XmaIC`CCGG,G1067
MluIA`CGCG,T4242  
 
 

The recommended sequencing primer sequences for pT7-N-His-WELQ plasmid are as follows:
Forward primer (4988-5006): 5'-TAATACGACTCACTATAGG-3'

Reverse primer (5551-5570): 5'-TGCTAGTTATTGCTCAGCGG-3'


Storage Conditions

Store at -20 °C.


Precautions

  • No commercial use of this plasmid is permitted without written permission from SBS Genetech. Nor shall it be transferred to any individual or institution outside the purchaser’s laboratory.
  • This product is intended solely for scientific‑research use by professionals. It shall not be adopted for clinical diagnosis or therapy, food‑related or pharmaceutical applications, nor kept in residential premises.
  • For your safety and health, wear a lab coat and disposable gloves during operation.

 

 

Only for research and not intended for treatment of humans or animals

 

 

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