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tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Products 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Enzymes
  • POCT 
    • Integrated POCT Platform
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Lateral Flow System
    • Freeze-Drying System
  • About 
    • About SBS
    • Solutions
    • Achievements
    • Legal Statement
  • Contact
  • …  
    • Home
    • Products 
      • All Products
      • Custom Services
      • Catalog Products
      • Innovative Systems
      • Nucleic Acid Related
      • Enzymes
    • POCT 
      • Integrated POCT Platform
      • LAMP
      • RPA
      • CRISPR
      • DNA-Free Enzymes
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      • Freeze-Drying System
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pRD29B-luc

pRD29B-luc

pRD29B-luc (plant reporter gene plasmid) is a reporter gene plasmid developed for transient transfection of firefly luciferase (firefly luciferase) protoplasts into plant (such as Arabidopsis thaliana, etc.).
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The main information of pRD29B-luc plasmid is as follows:

  • Feature Nucleotide
  • Position
  • Ampicillin resistance ORF
  • 137-997
  • Ampicillin promoter
  • 998-1102
  • M13 forward primer(M13 fwd)
  • 1576-1592
  • RD29B promoter
  • 1646-2226
  • Luciferase
  • 2229-3881
  • NOS terminator
  • 4036-4288
  • M13 reverse primer(M13 rev)
  • 4307-4323
  • lac operator
  • 4331-4347
  • lac promoter
  • 4355-4385
  • CAP binding site
  • 4400-4421
  • pUC origin
  • 4709-5297

The map of pRD29B-luc plasmid (5479bp) is as follows:

pRD29B-luc

Restriction enzymes that do not cut pRD29B-luc include:

Acc65IAccIAfeIAgeIAleIApaIAscI
AsiSIAvrIIBaeIBbvCIBclIBlpIBmtI
Bpu10IBsgIBsmIBstXIBstZ17IEagIFseI
HpaIKpnIMluIMscINaeINgoMIVNheI
NotINruIPaeR7IPflFIPflMIPmeIPmlI
PshAIPspOMIPspXIRsrIISacIISalISexAI
SfiISmaISnaBISpeISrfIStuISwaI
TspMITth111IXhoIXmaI


Restriction enzymes that cut pRD29B-luc once include:

AatIIG,ACGT`C1128DraIIICAC,NNN`GTG1884
AflIIA`CRYG,T4065EcoNICCTNN`N,NNAGG3846
AhdIGACNN,N`NNGTC211EcoRVGAT|ATC3567
AlwNICAG,NNN`CTG5061HincIIGTY|RAC3533
AvaIC`YCGR,G3285HindIIIA`AGCT,T1597
BamHIG`GATC,C1622NcoIC`CATG,G2228
BfuAIACCTGCNNNN`NNNN,3627NmeAIIIGCCGAG(N)19,NN`357
BglIIA`GATC,T4285NsiIA,TGCA`T4152
BmgBICAC|GTC2030PacITTA,AT`TAA3551
BsaBIGATNN|NNATC4099PpuMIRG`GWC,CY3408
BsaIGGTCTCN`NNNN,272PsiITTA|TAA2554
BseRIGAGGAG(N)8,NN`3782PstIC,TGCA`G1613
BsoBIC`YCGR,G3285SbfICC,TGCA`GG1608
BspMIACCTGCNNNN`NNNN,3627ScaIAGT|ACT691
BsrGIT`GTAC,A2719SgrAICR`CCGG,YG3657
BstAPIGCAN,NNN`NTGC1379StyIC`CWWG,G2227
BstEIIG`GTNAC,C2837XcmICCANNNN,N`NNNNTGG2963
Bsu36ICC`TNA,GG2842XmnIGAANN|NNTTC809
BtgIC`CRYG,G2227ZraIGAC|GTC1130
CspCI,NN`(N)11CAA(N)5GTGG(N)10,NN`1904


Restriction enzymes that cut pRD29B-luc once include:

M13 forward primer(1576-1592): 5'-GTAAAACGACGGCCAGT-3'

luc promoter primer(2558-2274): 5'-GATAGAATGGCGCCGGG-3'


Storage Conditions

Store at -20 °C.


Precautions

  • No commercial use of this plasmid is permitted without written permission from SBS Genetech. Nor shall it be transferred to any individual or institution outside the purchaser’s laboratory.
  • This product is intended solely for scientific‑research use by professionals. It shall not be adopted for clinical diagnosis or therapy, food‑related or pharmaceutical applications, nor kept in residential premises.
  • For your safety and health, wear a lab coat and disposable gloves during operation.

 

 

Only for research and not intended for treatment of humans or animals

 

 

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