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tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Products 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Enzymes
  • POCT 
    • Integrated POCT Platform
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Lateral Flow System
    • Freeze-Drying System
  • About 
    • About SBS
    • Solutions
    • Achievements
    • Legal Statement
  • Contact
  • …  
    • Home
    • Products 
      • All Products
      • Custom Services
      • Catalog Products
      • Innovative Systems
      • Nucleic Acid Related
      • Enzymes
    • POCT 
      • Integrated POCT Platform
      • LAMP
      • RPA
      • CRISPR
      • DNA-Free Enzymes
      • Lateral Flow System
      • Freeze-Drying System
    • About 
      • About SBS
      • Solutions
      • Achievements
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pISRE-TA-luc

pISRE-TA-luc

pISRE-TA-luc (reporter gene plasmid) is a reporter gene plasmid independently developed by Beyuntian for detecting the level of ISRE transcription activity. pISRE-TA-luc uses our company pGL6-TA plasmid as a template and inserts multiple ISRE binding sites into its multiple cloning site, which can detect the activation level of ISRE with high sensitivity.
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More Details

All products have special prices for bulk purchase, please contact for more details if required.

 

 

The main information of pISRE-TA-luc plasmid is as follows:

  • Base pairs
  • 5671
  • ISRE response element
  • 26-85
  • Minimal TA promoter (pTA)
  • 108-130
  • luc2 reporter gene
  • 172-1824
  • SV40 late poly(A) signal
  • 1859-2080
  • SV40 early enhancer/promoter
  • 2128-2546
  • Synthetic neomycin phosphotransferase
  •  
  • (Neor) coding region
  • 2571-3365
  • Synthetic poly(A) signal
  • 3390-3438
  • Reporter Vector primer 4 (RVprimer4) binding region
  • 3505-3524
  • ColE1-derived plasmid replication origin
  • 3762
  • Synthetic Beta-lactamase (Ampr) coding region
  • 4553-5413
  • Synthetic poly(A) signal/transcriptional pause site
  • 5518-5671
  • Reporter Vector primer 3 (RVprimer3) binding region
  • 5620-5639

The map of pISRE-TA-luc plasmid is as follows:

pISRE-TA-luc

 

 

Restriction enzymes that do not cut pISRE-TA-luc include:

Aat IIAfl IIAsc IAse IBsa IBsaA IBsiW IBspM II
BssH IIEco72 IEcoR IEcoR VMlu INde INru IPaeR7 I
PflM IPme IPml IPsp1406 IPspA IRsr IISac ISma I
SnaB ISpl ISrf ITth111 IVsp IXcm IXho I
 
  

Restriction enzymes that cut pISRE-TA-luc once include:

Sfi IGGCCN,NNN`NGGCC9 BstB ITT`CG,AA3441
Bgl IGCCN,NNN`NGGC9 Sal IG`TCGA,C3455
Acc65 IG`GTAC,C15 Afl IIIA`CRYG,T3705
Asp718G`GTAC,C15 ApaL IG`TGCA,C4019
Kpn IG,GTAC`C19 HgiE IIACCNNNNNNGGT -1/134284
Nhe IG`CTAG,C21 Not IGC`GGCC,GC4525
Bgl IIA`GATC,T87 BstX ICCAN,NNNN`NTGG4549
Hind IIIA`AGCT,T97 BstE IIG`GTNAC,C4552
BsrG IT`GTAC,A663 Ahd IGACNN,N`NNGTC4627
Dra IIICAC,NNN`GTG1319 Bsu36 ICC`TNA,GG4983
Gsu ICTGGAG 21/191552 Pvu ICG,AT`CG4997
Bpm ICTGGAG 22/201553 Sac IICC,GC`GG5021
Apo IR`AATT,Y1935 Bst1107 IGTA|TAC5137
Mun IC`AATT,G1999 Xca IGTA|TAC5137
BamH IG`GATC,C2092 Xca IGTA|TAC5137
Stu IAGG|CCT2525 Spe IA`CTAG,T5456
EcoN ICCTNN`N,NNAGG3046 BsmA IGTCTC`/95468
BsiC ITT`CG,AA3441 BsmB ICGTCTC 7/115469


The recommended sequencing primer sequences for pISRE-TA-luc plasmid are as follows:

RVprimer3 (5620-5639):
GCA AAA TAG GCT GTC CC


Storage Conditions

Store at -20 °C.


Precautions

  • No commercial use of this plasmid is permitted without written permission from SBS Genetech. Nor shall it be transferred to any individual or institution outside the purchaser’s laboratory.
  • This product is intended solely for scientific‑research use by professionals. It shall not be adopted for clinical diagnosis or therapy, food‑related or pharmaceutical applications, nor kept in residential premises.
  • For your safety and health, wear a lab coat and disposable gloves during operation.

 

 

Only for research and not intended for treatment of humans or animals

 

 

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

 

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