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The main information of the pISRE-TA-GLuc-Dura plasmid is as follows:
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- Minimal TA promoter (pTA)
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- SV40 late poly (A) signal
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- SV40 early enhancer/promoter
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- Synthetic neomycin phosphotransferase (Neor) coding region
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- Synthetic poly (A) signal
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- Reporter Vector primer 4 (RVprimer4) binding region
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- ColE1-derived plasmid replication origin
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- Synthetic Beta-lactamase (Ampr) coding region
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- Synthetic poly (A) signal/transcriptional pause site
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- Reporter Vector primer 3 (RVprimer3) binding region
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The main information of the pISRE-TA-GLuc-Dura plasmid is as follows:
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Restriction enzymes that do not cut pISRE-TA-GLuc-Dura include:
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| AatII | AclI | AflII | AscI | AseI | AsiSI | AvaI | | BmgBI | BsaAI | BsaI | BsiWI | BsoBI | BspEI | BsrGI | | BssHII | CspCI | DraIII | Eco53kI | EcoRI | EcoRV | MluI | | NdeI | PacI | PaeR7I | PflFI | PflMI | PmeI | PmlI | | PspXI | RsrII | SacI | SbfI | SmaI | SnaBI | SrfI | | SwaI | TspMI | Tth111I | XcmI | XhoI | XmaI | ZraI |
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The single restriction enzyme sites in pISRE-TA-GLuc-Dura (Restriction enzymes that cut pISRE-TA-GLuc-Dura) include:
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| Acc65I | G`GTAC,C | 14 | BstXI | CCAN,NNNN`NTGG | 3449 | | AflIII | A`CRYG,T | 2609 | BstZ17I | GTA|TAC | 4041 | | AgeI | A`CCGG,T | 2280 | Bsu36I | CC`TNA,GG | 3887 | | AleI | CACNN|NNGTG | 3451 | EcoNI | CCTNN`N,NNAGG | 1950 | | ApaI | G,GGCC`C | 1546 | Esp3I | CGTCTCN`NNNN, | 4373 | | ApoI | R`AATT,Y | 839 | FspI | TGC|GCA | 1034 | | BaeI | ,(N)5`(N)10ACNNNNGTAYC(N)7,(N)5` | 1676 | HindIII | A`AGCT,T | 97 | | BamHI | G`GATC,C | 996 | HpaI | GTT|AAC | 894 | | BbvCI | CC`TCA,GC | 2161 | KpnI | G,GTAC`C | 19 | | BciVI | GTATCC(N)5,N` | 2811 | MfeI | C`AATT,G | 903 | | BcoDI | GTCTCN`NNNN, | 4373 | NheI | G`CTAG,C | 21 | | BglI | GCCN,NNN`NGGC | 9 | NotI | GC`GGCC,GC | 3429 | | BglII | A`GATC,T | 87 | NruI | TCG|CGA | 277 | | BmtI | G,CTAG`C | 20 | PciI | A`CATG,T | 2609 | | BpmI | CTGGAG(N)14,NN` | 350 | PsiI | TTA|TAA | 874 | | BsaXI | ,NNN`(N)9AC(N)5CTCC(N)7,NNN` | 164 | PspOMI | G`GGCC,C | 1546 | | BsmAI | GTCTCN`NNNN, | 4373 | PvuII | CAG|CTG | 1106 | | BsmBI | CGTCTCN`NNNN, | 4373 | SfiI | GGCCN,NNN`NGGCC | 5 | | BspHI | T`CATG,A | 3333 | SgrAI | CR`CCGG,YG | 1692 | | BssSI | C`ACGA,G | 2782 | SpeI | A`CTAG,T | 4360 | | BstBI | TT`CG,AA | 2345 | StuI | AGG|CCT | 1429 | | BstEII | G`GTNAC,C | 3456 | XmnI | GAANN|NNTTC | 433 |
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The sequencing primer sequences that can be used for the pISRE-TA-GLuc-Dura plasmid are as follows:
RVprimer3 (4525-4544): CTA GCA AAA TAG GCT GTC CC
Storage Conditions
Store at -20 °C.
Precautions
- No commercial use of this plasmid is permitted without written permission from SBS Genetech. Nor shall it be transferred to any individual or institution outside the purchaser’s laboratory.
- This product is intended solely for scientific‑research use by professionals. It shall not be adopted for clinical diagnosis or therapy, food‑related or pharmaceutical applications, nor kept in residential premises.
- For your safety and health, wear a lab coat and disposable gloves during operation.
Only for research and not intended for treatment of humans or animals
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