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tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Products 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Enzymes
  • POCT 
    • Integrated POCT Platform
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Lateral Flow System
    • Freeze-Drying System
  • About 
    • About SBS
    • Solutions
    • Achievements
    • Legal Statement
  • Contact
  • …  
    • Home
    • Products 
      • All Products
      • Custom Services
      • Catalog Products
      • Innovative Systems
      • Nucleic Acid Related
      • Enzymes
    • POCT 
      • Integrated POCT Platform
      • LAMP
      • RPA
      • CRISPR
      • DNA-Free Enzymes
      • Lateral Flow System
      • Freeze-Drying System
    • About 
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pGRE-luc

pGRE-luc

pGRE-luc (reporter gene plasmid) is a reporter gene plasmid independently developed by Beyuntian for detecting the transcription activation level of glucocorticoid response element (GRE). pGRE-luc uses our company pGL6 plasmid as a template and inserts multiple GRE sites into its multiple cloning site. It can detect the activation level of GRE with high sensitivity and detect the activation level of the glucocorticoid-mediated signaling pathway.
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More Details

All products have special prices for bulk purchase, please contact for more details if required.

 

 

The main information of pGRE-luc plasmid is as follows:

  • Base pairs
  • 5615
  • Glucocorticoid response element (GRE)
  • 26-70
  • luc2 reporter gene
  • 116-1768
  • SV40 late poly(A) signal
  • 1803-2024
  • SV40 early enhancer/promoter
  • 2072-2490
  • Synthetic neomycin phosphotransferase
  • (Neor) coding region
  • 2515-3309
  • Synthetic poly(A) signal
  • 3334-3382
  • Reporter Vector primer 4 (RVprimer4) binding region
  • 3449-3468
  • ColE1-derived plasmid replication origin
  • 3706
  • Synthetic Beta-lactamase (Ampr) coding region
  • 4497-5357
  • Synthetic poly(A) signal/transcriptional pause site
  • 5462-5615
  • Reporter Vector primer 3 (RVprimer3) binding region
  • 5564-5583

The map of pGRE-luc plasmid is as follows:

pGRE-luc

 

 

Restriction enzymes that do not cut pGRE-luc include:

Aat IIAfl IIAsc IAse IBsa IBsaA IBsiW IBspM II
BssH IIEco72 IEcoR IEcoR VMlu INde INru IPaeR7 I
PflM IPme IPml IPsp1406 IPspA IRsr IISac ISma I
SnaB ISpl ISrf ITth111 IVsp IXcm IXho IXma I
 
  

Restriction enzymes that cut pGRE-luc once include:

Sfi IGGCCN,NNN`NGGCC9 BsiC ITT`CG,AA3385
Bgl IGCCN,NNN`NGGC9 BstB ITT`CG,AA3385
Acc65 IG`GTAC,C15 Sal IG`TCGA,C3399
Asp718G`GTAC,C15 Afl IIIA`CRYG,T3649
Kpn IG,GTAC`C19 ApaL IG`TGCA,C3963
Nhe IG`CTAG,C21 Not IGC`GGCC,GC4469
Bgl IIA`GATC,T72 BstX ICCAN,NNNN`NTGG4493
Hind IIIA`AGCT,T82 BstE IIG`GTNAC,C4496
BsrG IT`GTAC,A607 Ahd IGACNN,N`NNGTC4571
Dra IIICAC,NNN`GTG1263 Bsu36 ICC`TNA,GG4927
Gsu ICTGGAG 21/191496 Pvu ICG,AT`CG4941
Bpm ICTGGAG 22/201497 Sac IICC,GC`GG4965
Apo IR`AATT,Y1879 Bst1107 IGTA|TAC5081
Mun IC`AATT,G1943 Xca IGTA|TAC5081
BamH IG`GATC,C2036 Spe IA`CTAG,T5400
Stu IAGG|CCT2469 BsmA IGTCTC`/95412
EcoN ICCTNN`N,NNAGG2990 BsmB ICGTCTC 7/115413


The recommended sequencing primer sequences for pGRE-luc plasmid are as follows:

RVprimer3(5564-5583):
 CTA GCA AAA TAG GCT GTC CC


Storage Conditions

Store at -20 °C.


Precautions

  • No commercial use of this plasmid is permitted without written permission from SBS Genetech. Nor shall it be transferred to any individual or institution outside the purchaser’s laboratory.
  • This product is intended solely for scientific‑research use by professionals. It shall not be adopted for clinical diagnosis or therapy, food‑related or pharmaceutical applications, nor kept in residential premises.
  • For your safety and health, wear a lab coat and disposable gloves during operation.

 

 

Only for research and not intended for treatment of humans or animals

 

 

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

 

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