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pGLuc-Dura-TA

pGLuc-Dura-TA

pGLuc-Dura-TA (reporter gene plasmid) is a new generation plasmid independently developed by our company for detection of secreted, highly stable, ATP-independent Gaussia-Dura Luciferase (Gluc-Dura) luciferase reporter gene in mammalian cells.
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The main information of pGLuc-Dura-TA plasmid is as follows:

  • Feature Nucleotide
  • Position
  • Multiple cloning region
  • 1-59
  • Minimal TA promoter (pTA)
  • 66-88
  • GLuc (MT) reporter gene
  • 130-687
  • SV40 late poly (A) signal
  • 718-939
  • SV40 early enhancer/promoter
  • 987-1405
  • Synthetic neomycin phosphotransferase (Neor) coding region
  • 1430-2224
  • Synthetic poly (A) signal
  • 2249-2297
  • Reporter Vector primer 4 (RVprimer4) binding region
  • 2364-2383
  • ColE1-derived plasmid replication origin
  • 2621
  • Synthetic Beta-lactamase (Ampr) coding region
  • 3412-4272
  • Synthetic poly (A) signal/transcriptional pause site
  • 4377-4530
  • Reporter Vector primer 3 (RVprimer3) binding region
  • 4479-4498

 

The map of pGLuc-Dura-TA plasmid (4530bp) is as follows:

pGLuc-Dura-TA

 

Restriction enzymes that do not cut pGLuc-Dura-TA include:

AatIIAclIAflIIAscIAsiSIBmgBIBpmI
BsaAIBsaIBsiWIBspEIBsrGIBssHIICspCI
DraIIIEcoRIEcoRVNdeIPacIPflFIPflMI
PmeIPmlIRsrIISbfISmaISnaBISrfI
SwaITspMITth111IXcmIXmaIZraI
  

The single restriction enzyme cleavage sites (Restriction enzymes that cut pGLuc-Dura-TA) in pGLuc-Dura-TA include:

Acc65IG`GTAC,C14Eco53kIGAG|CTC34
AgeIA`CCGG,T2234EcoNICCTNN`N,NNAGG1904
AleICACNN|NNGTG3405Esp3ICGTCTCN`NNNN,4327
ApaIG,GGCC`C1500FspITGC|GCA988
ApoIR`AATT,Y793HindIIIA`AGCT,T55
AvaIC`YCGR,G26HpaIGTT|AAC848
BaeI,(N)5`(N)10ACNNNNGTAYC(N)7,(N)5`1630KpnIG,GTAC`C19
BamHIG`GATC,C950MfeIC`AATT,G857
BbvCICC`TCA,GC2115MluIA`CGCG,T39
BciVIGTATCC(N)5,N`2765NheIG`CTAG,C21
BcoDIGTCTCN`NNNN,4327NotIGC`GGCC,GC3383
BglIGCCN,NNN`NGGC9NruITCG|CGA235
BglIIA`GATC,T45PaeR7IC`TCGA,G26
BmtIG,CTAG`C20PciIA`CATG,T2563
BpmICTGGAG(N)14,NN`308PsiITTA|TAA828
BsaXI,NNN`(N)9AC(N)5CTCC(N)7,NNN`122PspOMIG`GGCC,C1500
BsmAIGTCTCN`NNNN,4327PspXIVC`TCGA,GB26
BsmBICGTCTCN`NNNN,4327PvuIICAG|CTG1060
BsoBIC`YCGR,G26SacIG,AGCT`C37
BspHIT`CATG,A3283SfiIGGCCN,NNN`NGGCC5
BssSIC`ACGA,G2736SgrAICR`CCGG,YG1646
BstBITT`CG,AA2299SpeIA`CTAG,T4314
BstEIIG`GTNAC,C3410StuIAGG|CCT1383
BstXICCAN,NNNN`NTGG3403XhoIC`TCGA,G27
BstZ17IGTA|TAC3995XmnIGAANN|NNTTC391
Bsu36ICC`TNA,GG3841  
 
 

The sequencing primer sequences that can be used for pGLuc-Dura-TA plasmid are as follows:

RVprimer3 (4479-4498): CTA GCA AAA TAG GCT GTC CC


Storage Conditions

Store at -20 °C.


Precautions

  • No commercial use of this plasmid is permitted without written permission from SBS Genetech. Nor shall it be transferred to any individual or institution outside the purchaser’s laboratory.
  • This product is intended solely for scientific‑research use by professionals. It shall not be adopted for clinical diagnosis or therapy, food‑related or pharmaceutical applications, nor kept in residential premises.
  • For your safety and health, wear a lab coat and disposable gloves during operation.

 

 

Only for research and not intended for treatment of humans or animals

 

 

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

 

 

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

 

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