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The main information of pGLuc plasmid is as follows:
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- SV40 late poly (A) signal
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- SV40 early enhancer/promoter
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- Synthetic neomycin phosphotransferase (Neor) coding region
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- Synthetic poly (A) signal
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- Reporter Vector primer 4 (RVprimer4) binding region
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- ColE1-derived plasmid replication origin
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- Synthetic Beta-lactamase (Ampr) coding region
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- Synthetic poly (A) signal/transcriptional pause site
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- Reporter Vector primer 3 (RVprimer3) binding region
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The map of pGLuc plasmid (4489bp) is as follows:
Restriction enzymes that do not cut pGLuc include:
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| AatII | AclI | AflII | AscI | AsiSI | BmgBI | BpmI | | BsaAI | BsaI | BsiWI | BspEI | BssHII | CspCI | DraIII | | EcoRI | EcoRV | NdeI | NruI | PacI | PflFI | PflMI | | PmeI | PmlI | RsrII | SbfI | SmaI | SnaBI | SrfI | | SwaI | TspMI | Tth111I | XcmI | XmaI | XmnI | ZraI |
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The single restriction enzyme cleavage sites (Restriction enzymes that cut pGLuc) in pGLuc include:
| Acc65I | G`GTAC,C | 14 | Eco53kI | GAG|CTC | 34 | | AgeI | A`CCGG,T | 2193 | EcoNI | CCTNN`N,NNAGG | 1863 | | AhdI | GACNN,N`NNGTC | 3443 | Esp3I | CGTCTCN`NNNN, | 4286 | | AleI | CACNN|NNGTG | 3364 | FspI | TGC|GCA | 947 | | ApaI | G,GGCC`C | 1459 | HindIII | A`AGCT,T | 55 | | AseI | AT`TA,AT | 643 | HpaI | GTT|AAC | 807 | | AvaI | C`YCGR,G | 26 | KpnI | G,GTAC`C | 19 | | BaeI | ,(N)5`(N)10ACNNNNGTAYC(N)7,(N)5` | 1589 | MfeI | C`AATT,G | 816 | | BamHI | G`GATC,C | 909 | MluI | A`CGCG,T | 39 | | BbvCI | CC`TCA,GC | 2074 | NotI | GC`GGCC,GC | 3342 | | BciVI | GTATCC(N)5,N` | 2724 | PaeR7I | C`TCGA,G | 26 | | BglI | GCCN,NNN`NGGC | 9 | PciI | A`CATG,T | 2522 | | BglII | A`GATC,T | 45 | PsiI | TTA|TAA | 787 | | BsaBI | GATNN|NNATC | 908 | PspOMI | G`GGCC,C | 1459 | | BsaXI | ,NNN`(N)9AC(N)5CTCC(N)7,NNN` | 81 | PspXI | VC`TCGA,GB | 26 | | BsmBI | CGTCTCN`NNNN, | 4286 | PvuI | CG,AT`CG | 3812 | | BsoBI | C`YCGR,G | 26 | PvuII | CAG|CTG | 1019 | | BspHI | T`CATG,A | 3242 | SacI | G,AGCT`C | 37 | | BsrGI | T`GTAC,A | 332 | SacII | CC,GC`GG | 3836 | | BssSI | C`ACGA,G | 2695 | SalI | G`TCGA,C | 2272 | | BstBI | TT`CG,AA | 2258 | SfiI | GGCCN,NNN`NGGCC | 5 | | BstEII | G`GTNAC,C | 3369 | SgrAI | CR`CCGG,YG | 1605 | | BstXI | CCAN,NNNN`NTGG | 3362 | SpeI | A`CTAG,T | 4273 | | BstZ17I | GTA|TAC | 3954 | StuI | AGG|CCT | 1342 | | Bsu36I | CC`TNA,GG | 3800 | XhoI | C`TCGA,G | 27 |
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The sequencing primer sequences that can be used for pGLuc plasmid are as follows:
RVprimer3 (4438-4457): CTA GCA AAA TAG GCT GTC CC
Storage Conditions
Store at -20 °C.
Precautions
- No commercial use of this plasmid is permitted without written permission from SBS Genetech. Nor shall it be transferred to any individual or institution outside the purchaser’s laboratory.
- This product is intended solely for scientific‑research use by professionals. It shall not be adopted for clinical diagnosis or therapy, food‑related or pharmaceutical applications, nor kept in residential premises.
- For your safety and health, wear a lab coat and disposable gloves during operation.
Only for research and not intended for treatment of humans or animals
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