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tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Products 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Enzymes
  • POCT 
    • Integrated POCT Platform
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Lateral Flow System
    • Freeze-Drying System
  • About 
    • About SBS
    • Solutions
    • Achievements
    • Legal Statement
  • Contact
  • …  
    • Home
    • Products 
      • All Products
      • Custom Services
      • Catalog Products
      • Innovative Systems
      • Nucleic Acid Related
      • Enzymes
    • POCT 
      • Integrated POCT Platform
      • LAMP
      • RPA
      • CRISPR
      • DNA-Free Enzymes
      • Lateral Flow System
      • Freeze-Drying System
    • About 
      • About SBS
      • Solutions
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Beijing SBS Genetech Co.,Ltd.
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pGL6-TA

pGL6-TA

pGL6-TA (reporter gene plasmid) is a new generation plasmid independently developed by our company for detection of firefly luciferase (also known as luciferase) reporter gene in mammalian cells.
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More Details

All products have special prices for bulk purchase, please contact for more details if required.

 

 

The main information of pGL6-TA plasmid is as follows:

  • Base pairs
  • 5629
  • Multiple cloning region
  • 1-59
  • Minimal TA promoter (pTA)
  • 66-88
  • luc2 reporter gene
  • 120-1782
  • SV40 late poly(A) signal
  • 1817-2038
  • SV40 early enhancer/promoter
  • 2086-2504
  • Synthetic neomycin phosphotransferase(Neor)coding region
  • 2529-3323
  • Synthetic poly(A) signal
  • 3348-3396
  • Reporter Vector primer 4 (RVprimer4) binding region
  • 3463-3482
  • ColE1-derived plasmid replication origin
  • 3720
  • Synthetic Beta-lactamase (Ampr) coding region
  • 4511-5371
  • Synthetic poly(A) signal/transcriptional pause site
  • 5476-5629
  • Reporter Vector primer 3 (RVprimer3) binding region
  • 5578-5597

 

The map of pGL6-TA plasmid is as follows:

pGL6-TA

 

Restriction enzymes that do not cut pGL6-TA include:

Aat IIAfl IIAsc IAse IBsa IBsaA IBsiW I
BspM IIBssH IIEco72 IEcoR IEcoR VNde INru I
PflM IPme IPml IPsp1406 IPspA IRsr IISma I
SnaB ISpl ISrf ITth111 I
  

Restriction enzymes that cut pGL6-TA once include:

SfiIGGCCN,NNN`NGGCC9 Stu IAGG|CCT2483
BglIGCCN,NNN`NGGC9 EcoN ICCTNN`N,NNAGG3004
Acc65 IG`GTAC,C15 BsiC ITT`CG,AA3399
Asp718G`GTAC,C15 BstB ITT`CG,AA3399
Kpn IG,GTAC`C19 Sal IG`TCGA,C3413
Nhe IG`CTAG,C21 ApaL IG`TGCA,C3977
PaeR7IC`TCGA,G27 HgiE IIACCNNNNNNGGT -1/134242
Xho IC`TCGA,G27 Not IGC`GGCC,GC4483
Sac IG,AGCT`C37 BstX ICCAN,NNNN`NTGG4507
Mlu IA`CGCG,T39 BstE IIG`GTNAC,C4510
Bgl IIA`GATC,T45 Ahd IGACNN,N`NNGTC4585
Hind IIIA`AGCT,T55 Bsu36 ICC`TNA,GG4941
BsrG IT`GTAC,A621 Pvu ICG,AT`CG4955
Dra IIICAC,NNN`GTG1277 Sac IICC,GC`GG4979
Gsu ICTGGAG 21/191510 Bst1107 IGTA|TAC5095
Bpm ICTGGAG 22/201511 Xca IGTA|TAC5095
Apo IR`AATT,Y1893 Spe IA`CTAG,T5414
Mun IC`AATT,G1957 BsmA IGTCTC`/95426
BamH IG`GATC,C2050 BsmB ICGTCTC 7/115427
  
 
 

The recommended sequencing primer sequences for pGL6-TA plasmid are as follows:

  RVprimer3(5578-5597):
  CTA GCA AAA TAG GCT GTC CC


Storage Conditions

Store at -20 °C.


Precautions

  • No commercial use of this plasmid is permitted without written permission from SBS Genetech. Nor shall it be transferred to any individual or institution outside the purchaser’s laboratory.
  • This product is intended solely for scientific‑research use by professionals. It shall not be adopted for clinical diagnosis or therapy, food‑related or pharmaceutical applications, nor kept in residential premises.
  • For your safety and health, wear a lab coat and disposable gloves during operation.

 

 

Only for research and not intended for treatment of humans or animals

 

 

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

 

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