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      • DNA-Free Enzymes
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    • About 
      • About SBS
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tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Products 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Enzymes
  • POCT 
    • Integrated POCT Platform
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Lateral Flow System
    • Freeze-Drying System
  • About 
    • About SBS
    • Solutions
    • Achievements
    • Legal Statement
  • Contact
  • …  
    • Home
    • Products 
      • All Products
      • Custom Services
      • Catalog Products
      • Innovative Systems
      • Nucleic Acid Related
      • Enzymes
    • POCT 
      • Integrated POCT Platform
      • LAMP
      • RPA
      • CRISPR
      • DNA-Free Enzymes
      • Lateral Flow System
      • Freeze-Drying System
    • About 
      • About SBS
      • Solutions
      • Achievements
      • Legal Statement
    • Contact
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Beijing SBS Genetech Co.,Ltd.
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pGL6-miR

pGL6-miR

pGL6-miR (reporter gene plasmid) is a luciferase reporter gene plasmid independently developed by our company for microRNA (miRNA) and other research.
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More Details

All products have special prices for bulk purchase, please contact for more details if required.

 

 

The main information of pGL6-miR plasmid is as follows:

  • Base pairs
  • 6097bp
  • Feature Nucleotide
  • Position
  • PGK promoter
  • 20-538
  • luc2 reporter gene
  • 549-2202
  • Multiple cloning region
  • 2208-2261
  • SV40 late poly(A) sinal
  • 2268-2512
  • SV40 early enhancer/promoter
  • 2554-2972
  • Synthetic neomycin phosphotransferase(Neor)coding region
  • 2998-3792
  • Synthetic poly(A) signal
  • 3817-3865
  • Synthetic Beta-lactamase(Ampr) coding region
  • 4980-5840
  • Synthetic poly(A) signal/transcriptional pause site
  • 5945-6097

The pGL6-miR plasmid map is as follows:

pGL6-miR

 

Restriction enzymes that do not cut pGL6-miR include:

Aat IIAfl IIAsc IAse IBsa IBsaA IBsiW I
BssH IIEco72 IEcoR INde INru IPflM IPme I
Pml IPsp1406 IPspA IRsr IISam ISnaB ISpl I
Srf ITth111 IVsp IXcm I
  

Restriction enzymes that cut pGL6-miR once include:

Sfi IGGCCN,NNN`NGGCC9 Sal IG`TCGA,C3881
BspM IIT`CCGG,A445 EcoN ICCTNN`N,NNAGG3472
BsaB IGATNN|NNATC997 Xba IT`CTAG,A3796
BsrG IT`GTAC,A1040 BsiC ITT`CG,AA3867
Dra IIICAC,NNN`GTG1696 BstB ITT`CG,AA3867
Acc65 IG`GTAC,C2209 ApaL IG`TGCA,C4445
Asp718G`GTAC,C2209 Ple IGAGTC 9/104510
Kpn IG,GTAC`C2213 HgiE IIACCNNNNNNGGT -1/134710
BamH IG`GATC,C2221 Not IGC`GGCC,GC4951
EcoR VGAT|ATC2229 BstX ICCAN,NNNN`NTGG4975
PaeR7 IC`TCGA,G2233 Pst IC,TGCA`G4975
Xho IC`TCGA,G2233 BstE IIG`GTNAC,C4978
Sac IG,AGCT`C243 Ahd IGACNN,N`NNGTC5053
Mlu IA`CGCG,T2245 Bsu36 ICC`TNA,GG5409
Bgl IIA`GATC,T2251 Pvu ICG,AT`CG5423
HinD IIIA`AGCT,T2257 Sac IICC,GC`GG5447
Apo IR`AATT,Y2361 Bst1107 IGTA|TAC5563
Mun IC`AATT,G2425 Xca IGTA|TAC5563
  
 
 

The recommended sequencing primer sequences for pGL6-miR plasmid are as follows:

   Forward primer (2135-2154): 5′GTTGGACGCCCGCAAGATCC 3′
   Reverse primer (2303-2322): 5′GGTTTGTCCAAACTCATCAA 3′


Storage Conditions

Store at -20 °C.


Precautions

  • No commercial use of this plasmid is permitted without written permission from SBS Genetech. Nor shall it be transferred to any individual or institution outside the purchaser’s laboratory.
  • This product is intended solely for scientific‑research use by professionals. It shall not be adopted for clinical diagnosis or therapy, food‑related or pharmaceutical applications, nor kept in residential premises.
  • For your safety and health, wear a lab coat and disposable gloves during operation.

 

 

Only for research and not intended for treatment of humans or animals

 

 

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

 

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