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tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Products 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Enzymes
  • POCT 
    • Integrated POCT Platform
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Lateral Flow System
    • Freeze-Drying System
  • About 
    • About SBS
    • Solutions
    • Achievements
    • Legal Statement
  • Contact
  • …  
    • Home
    • Products 
      • All Products
      • Custom Services
      • Catalog Products
      • Innovative Systems
      • Nucleic Acid Related
      • Enzymes
    • POCT 
      • Integrated POCT Platform
      • LAMP
      • RPA
      • CRISPR
      • DNA-Free Enzymes
      • Lateral Flow System
      • Freeze-Drying System
    • About 
      • About SBS
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pGL6

pGL6

pGL6 (reporter gene plasmid) is a new generation plasmid independently developed by our company for detection of firefly luciferase reporter gene in mammalian cells. This reporter gene plasmid has been comprehensively improved over Promega's pGL3 series. On the one hand, the encoding of luciferase has been improved to ensure better expression in mammalian cells. At the same time, all predicted possible transcription factor binding sites in the entire plasmid have been appropriately mutated, minimizing the non-specific binding of various transcription factors on the plasmid while maintaining the original function.
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More Details

All products have special prices for bulk purchase, please contact for more details if required.

 

 

The main information of pGL6 plasmid is as follows:

  • Base pairs
  • 5588
  • Multiple cloning region
  • 1-59
  • luc2 reporter gene
  • 89-1741
  • SV40 late poly(A) signal
  • 1776-1997
  • SV40 early enhancer/promoter
  • 2045-2463
  • Synthetic neomycin phosphotransferase(Neor)coding region
  • 2488-3282
  • Synthetic poly(A) signal
  • 3307-3355
  • Reporter Vector primer 4 (RVprimer4) binding region
  • 3422-3441
  • ColE1-derived plasmid replication origin
  • 3679
  • Synthetic Beta-lactamase (Ampr) coding region
  • 4470-5330
  • Synthetic poly(A) signal/transcriptional pause site
  • 5435-5588
  • Reporter Vector primer 3 (RVprimer3) binding region
  • 5537-5556

 

The map of pGL6 plasmid is as follows:

pGL6

 

Restriction enzymes that do not cut pGL6 include:

Aat IIAfl IIAsc IAse IBsa IBsaA IBsiW I
BspM IIBssH IIEco72 IEcoR IEcoR VNde INru I
PflM IPme IPml IPsp1406 IPspA IRsr II
Sma ISnaB ISpl ISrf ITth111 I
  

Restriction enzymes that cut pGL6 once include:

Sfi IGGCCN,NNN`NGGCC9 Stu IAGG|CCT2442
Bgl IGCCN,NNN`NGGC9 EcoN ICCTNN`N,NNAGG2963
Acc65 IG`GTAC,C15 BsiC ITT`CG,AA3358
Asp718G`GTAC,C15 BstB ITT`CG,AA3358
Kpn IG,GTAC`C19 Sal IG`TCGA,C3372
Nhe IG`CTAG,C21 ApaL IG`TGCA,C3936
PaeR7 IC`TCGA,G27 HgiE IIACCNNNNNNGGT -1/134201
Xho IC`TCGA,G27 Not IGC`GGCC,GC4442
Sac IG,AGCT`C37 BstX ICCAN,NNNN`NTGG4466
Mlu IA`CGCG,T39 BstE IIG`GTNAC,C4469
Bgl IIA`GATC,T45 Ahd IGACNN,N`NNGTC4544
Hind IIIA`AGCT,T55 Bsu36 ICC`TNA,GG4900
BsrG IT`GTAC,A580 Pvu ICG,AT`CG4914
Dra IIICAC,NNN`GTG1236 Sac IICC,GC`GG4938
Gsu ICTGGAG 21/191469 Bst1107 IGTA|TAC5054
Bpm ICTGGAG 22/201470 Xca IGTA|TAC5054
Apo IR`AATT,Y1852 Spe IA`CTAG,T5373
Mun IC`AATT,G1916 BsmA IGTCTC`/95385
BamH IG`GATC,C2009 BsmB ICGTCTC 7/115386
 

The recommended sequencing primer sequences for pGL6 plasmid are as follows:

RVprimer3(5537-5556):
CTA GCA AAA TAG GCT GTC CC


Storage Conditions

Store at -20 °C.


Precautions

  • No commercial use of this plasmid is permitted without written permission from SBS Genetech. Nor shall it be transferred to any individual or institution outside the purchaser’s laboratory.
  • This product is intended solely for scientific‑research use by professionals. It shall not be adopted for clinical diagnosis or therapy, food‑related or pharmaceutical applications, nor kept in residential premises.
  • For your safety and health, wear a lab coat and disposable gloves during operation.

 

 

Only for research and not intended for treatment of humans or animals

 

 

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

 

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