pET-N-His-SUMO3-Avi
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The main information of pET-N-His-SUMO3-Avi plasmid is as follows:
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The map of pET-N-His-SUMO3-Avi plasmid (5759bp) is as follows:
Restriction sites not found in pET-N-His-SUMO3-Avi include:
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The single enzyme cutting sites in pET-N-His-SUMO3-Avi include:
| AhdI | GACNN,N`NNGTC | 2049 | HpaI | GTT|AAC | 4536 |
| AlwNI | CAG,NNN`CTG | 2528 | MluI | A`CGCG,T | 5038 |
| ApaI | G,GGCC`C | 4835 | NcoI | C`CATG,G | 106 |
| AvaI | C`YCGR,G | 502 | NotI | GC`GGCC,GC | 494 |
| BamHI | G`GATC,C | 411 | PaeR7I | C`TCGA,G | 502 |
| BclI | T`GATC,A | 5024 | PciI | A`CATG,T | 2937 |
| BglII | A`GATC,T | 1 | PflFI | GACN`N,NGTC | 3195 |
| BmgBI | CAC|GTC | 389 | PflMI | CCAN,NNN`NTGG | 5463 |
| Bpu10I | CC`TNA,GC | 3832 | PpuMI | RG`GWC,CY | 3932 |
| BseRI | GAGGAG 16/14 | 159 | PshAI | GACNN|NNGTC | 4197 |
| BsoBI | C`YCGR,G | 502 | PsiI | TTA|TAA | 1034 |
| BspQI | GCTCTTCN`NNN, | 3054 | PspOMI | G`GGCC,C | 4831 |
| BssHII | G`CGCG,C | 4627 | PspXI | VC`TCGA,GB | 502 |
| BstAPI | GCAN,NNN`NTGC | 5362 | PstI | C,TGCA`G | 1807 |
| BstBI | TT`CG,AA | 433 | PvuI | CG,AT`CG | 1680 |
| BstEII | G`GTNAC,C | 4856 | SacI | G,AGCT`C | 478 |
| BstZ17I | GTA|TAC | 3170 | SalI | G`TCGA,C | 481 |
| DraIII | CAC,NNN`GTG | 909 | SapI | GCTCTTCN`NNN, | 3054 |
| EagI | C`GGCC,G | 494 | ScaI | AGT|ACT | 1568 |
| Eco53kI | GAG|CTC | 476 | SgrAI | CR`CCGG,YG | 5719 |
| EcoNI | CCTNN`N,NNAGG | 5506 | SphI | G,CATG`C | 5571 |
| EcoRI | G`AATT,C | 468 | StuI | AGG|CCT | 270 |
| EcoRV | GAT|ATC | 4592 | Tth111I | GACN`N,NGTC | 3195/td> |
| FspAI | TGC|GCA | 3960 | XbaI | T`CTAG,A | 67 |
| HindIII | A`AGCT,T | 487 | XhoI | C`TCGA,G | 502 |
The recommended sequencing primer sequences for pET-N-His-SUMO3-Avi plasmid are as follows:
T7-R (21-39): 5'-CCCTATAGTGAGTCGTATTA-3'
Storage Conditions
Store at -20 °C.
Precautions
- No commercial use of this plasmid is permitted without written permission from SBS Genetech. Nor shall it be transferred to any individual or institution outside the purchaser’s laboratory.
- This product is intended solely for scientific‑research use by professionals. It shall not be adopted for clinical diagnosis or therapy, food‑related or pharmaceutical applications, nor kept in residential premises.
- For your safety and health, wear a lab coat and disposable gloves during operation.
Only for research and not intended for treatment of humans or animals
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