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pET-N-Avi-His-SUMO3

pET-N-Avi-His-SUMO3

pET-N-Avi-His-SUMO3 is a prokaryotic expression plasmid used to express target proteins that can be labeled with biotin in Escherichia coli (E.coli). This plasmid drives the target protein with Avi tag (Avi tag), His tag (His tag) and SUMO3 tag (SUMO3 tag) at the N terminus from the T7 promoter. Under in vitro conditions in the presence of ATP, biotin (Biotin), and biotin ligase (Biotin ligase) BirA, BirA catalyzes the covalent binding of biotin to the Avi tag at the N-terminus of the target protein, thereby biotin-labeling the target protein. At the same time, the plasmid contains a SUMO3 tag, which can be specifically recognized and cleaved by rSENP2 protease, thereby achieving efficient separation of the corresponding tagged protein and the target protein. This plasmid is ampicillin resistant.
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The main information of pET-N-Avi-His-SUMO3 plasmid is as follows:

 

  • Feature Nucleotide
  • Position
  • T7 promoter
  • 21-39
  • Avi tag
  • 120-164
  • His tag
  • 180-197
  • SUMO3 tag
  • 204-479
  • Multiple Cloning Sites
  • 477-502
  • His tag
  • 523-540
  • T7 Terminator
  • 607-654
  • f1 origin
  • 691-1146
  • Ampicillin
  • 1277-2137
  • ori
  • 2308-2896
  • lacI
  • 4326-5408

 

The map of pET-N-Avi-His-SUMO3 plasmid (5774bp) is as follows:

pET-N-Avi-His-SUMO3

 

Restriction sites not found in pET-N-Avi-His-SUMO3 include:
 

AarIAatIIAbsIAcc36IAcvIAflIIAgeI
AhlIAjuIAjuIAscIAsiGIAsiSIAspA2I
AsuIIAsuNHIAvrIIAxyIBaeIBalIBanIII
BbrPIBbvCIBcuIBfrIBfuAIBlnIBmtI
BplIBpu14IBsaIBsa29IBsaMIBse21IBseCI
BshVIBshTIBsiWIBsmIBso31IBsp68IBsp119I
Bsp1407IBspDIBspMIBspOIBspTIBspT104IBspTNI
BspXIBsrGIBst98IBstAFIBstAUIBstBIBstSNI
Bsu15IBsu36IBsuTUIBtuMIBveICfr9ICfr42I
ClaICpoICsiICspICsp45ICspAIEco31I
Eco72IEco81IEco105IEcoT22IFalIFauNDIFseI
I-CeuII-PpoII-SceIKflIKspIMabIMauBI
MfeIMlsIMluNIMph1103IMreIMscIMsp20I
MspCIMssIMunIMva1269INb.BbvCINb.BsmINb.Mva1269
NdeINheINruINsiINspVNt.BbvCIOliI
PacIPalAIPasIPctIPfl23IIPI-PspIPI-SceI
PinAIPmaCIPmeIPmlIPspCIPspLIPsrI
RgaIRigIRruIRsrIIRsr2ISacIISanDI
SbfISdaISexAISfaAISfiISfr303ISfuI
SgfISgrBISgrDISgsISmaISmiISnaBI
SpeISrfISse8387ISspBISstIISwaITspMI
Vha464IXmaIXmaCIXmaJIZraIZsp2I
 

The single enzyme cleavage sites in pET-N-Avi-His-SUMO3 include:
 

Acc65IG`GTAC,C168KpnIG`GCGC,C172
AhdIGACNN,N`NNGTC2064MluIA`CGCG,T5053
AlwNICAG,NNN`CTG2543NotIGC`GGCC,GC509
ApaIG,GGCC`C4850PaeR7IC`TCGA,G517
AvaIC`YCGR,G517PciIA`CATG,T2952
BamHIG`GATC,C477PflFIGACN`N,NGTC3210
BclIT`GATC,A5039PflMICCAN,NNN`NTGG5478
BglIIA`GATC,T1PpuMIRG`GWC,CY/td>3947
BmgBICAC|GTC455PshAIGACNN|NNGTC4212
BseRIGAGGAG 16/14225PsiITTA|TAA1049
BsoBIC`YCGR,G517PspOMIG`GGCC,C4846
BspQIGCTCTTCN`NNN,3069PspXIVC`TCGA,GB517
BssHIIG`CGCG,C4642PstIC,TGCA`G1822
BstAPIGCAN,NNN`NTGC5377PvuICG,AT`CG1695
BstEIIG`GTNAC,C4871SacIG,AGCT`C493
BstZ17IGTA|TAC3185SalIG`TCGA,C496
DraIIICAC,NNN`GTG924SapIGCTCTTCN`NNN,3069
EagIC`GGCC,G509ScaIAGT|ACT1583
Eco53kIGAG|CTC491SgrAICR`CCGG,YG5734
EcoNICCTNN`N,NNAGG5521SphIG,CATG`C5586
EcoRIG`AATT,C483StuIAGG|CCT336
EcoRVGAT|ATC4607Tth111IGACN`N,NGTC3210
HindIIIA`AGCT,T502XbaIT`CTAG,A67
HpaIGTT|AAC4551XhoIC`TCGA,G517
 
 

The recommended sequencing primer sequences for pET-N-Avi-His-SUMO3 plasmid are as follows:
T7-R (21-40): 5'-CCCTATAGTGAGTCGTATTA-3'

Storage Conditions

Store at -20 °C.


Precautions

  • No commercial use of this plasmid is permitted without written permission from SBS Genetech. Nor shall it be transferred to any individual or institution outside the purchaser’s laboratory.
  • This product is intended solely for scientific‑research use by professionals. It shall not be adopted for clinical diagnosis or therapy, food‑related or pharmaceutical applications, nor kept in residential premises.
  • For your safety and health, wear a lab coat and disposable gloves during operation.

 

 

Only for research and not intended for treatment of humans or animals

 

 

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

 

 

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

 

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