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pET-His-MCS-Avi

pET-His-MCS-Avi

pET-His-MCS-Avi is a self-developed prokaryotic expression plasmid for expressing target proteins that can be labeled with biotin in Escherichia coli (E.coli). The plasmid is located between the His tag and the Avi tag at the Multiple Cloning Sites (MCS). After the target protein is expressed, under in vitro conditions in the presence of ATP, biotin (Biotin), and biotin ligase (Biotin ligase) BirA, BirA catalyzes the covalent binding of biotin to the Avi tag at the N-terminus of the target protein, thereby biotin-labeling the target protein. This plasmid is ampicillin resistant.
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The main information of pET-His-MCS-Avi plasmid is as follows:

 

  • Feature Nucleotide
  • Position
  • Ampicillin
  • 209-1069
  • ori
  • 1240-1828
  • lacI
  • 3761-4843
  • T7 promoter
  • 5230-5248
  • His tag
  • 5323-5352
  • Multiple Cloning Sites
  • 5378-5414
  • Avi tag
  • 5428-5472
  • T7 Terminator
  • 5540-5587

 

 

The map of pET-His-MCS-Avi plasmid (5799bp) is as follows:

 

pET-His-MCS-Avi

 

 

Restriction sites not found in pET-His-MCS-Avi include:

       
AanIAarIAatIAbsIAcvIAdeIAflII
AhlIAjiIAjuIAleIAloIAscIAsiSI
AspA2IAsuIIAsuNHIAvrIIAxyIBaeIBbrPI
BbvCIBcuIBfrIBlnIBmgBIBmtIBplI
Bpu14IBse21IBseRIBsiWIBsp119IBsp1407IBspOI
BspTIBspT104IBsrGIBst98IBstAFIBstAUIBstBI
BstSNIBsu36IBtrICfr9ICpoICsiICspI
Csp45ICspCIDraIIIEcl136IIEco53kIEco72IEco81I
Eco105IEco147IEcoICRIEcoT22IFalIFseII-CeuI
I-PpoII-SceIKflIMabIMauBIMfeIMph1103I
MreIMspCIMssIMunINb.BbvCINheINsiI
NspVNt.BbvCIOliIPacIPalAIPasIPceI
Pfl23IIPI-PspIPI-SceIPmaCIPmeIPmlIPsiI
Psp124BIPspCIPspLIPsrIRgaIRigIRsrII
Rsr2ISacISalISanDISbfISdaISexAI
SfaAISfiISfuISgfISgrDISgsISmaI
SmiISnaBISpeISrfISse8387ISseBISspBI
SstIStuISwaITspMIVha464IXmaIXmaCI
XmaJIZsp2I    


The single enzyme cutting sites in pET-N-His-Avi include:
 

AatIIG,ACGT`C77KpnIG,GTAC`C5398
Acc65IG`GTAC,C2116MluIA`CGCG,T4488
AccIGT`MK,AC5394MscITGG|CCA2917
AgeIA`CCGG,T5409NcoIC`CATG,G5315
AhdIGACNN,N`NNGTC996NdeICA`TA,TG5378
AlwNICAG,NNN`CTG1475NotIGC`GGCC,GC5401
ApaIG,GGCC`C4285NruITCG|CGA3389
BclIT`GATC,A4474PaeR7IC`TCGA,G5383
BfuAIACCTGCNNNN`NNNN,3305PciIA`CATG,T1884
BglIIA`GATC,T5210PflFIGACN`N,NGTC2142
BlpIGC`TNA,GC5529PshAIGACNN|NNGTC3647
Bpu10ICC`TNA,GC2779PspOMIG`GGCC,C4281
BsaAIYAC|GTR930PspXIVC`TCGA,GB5383
BsaIGGTCTC 7/112136PstIC,TGCA`G754
BspMIACCTGCNNNN`NNNN,3305PvuICG,AT`CG627
BspQIGCTCTTCN`NNN,2001SacIICC,GC`GG5401
BssHIIG`CGCG,C4077SapIGCTCTTCN`NNN,2001
BstEIIG`GTNAC,C4306ScaIAGT|ACT515
BstZ17IGTA|TAC2117SgrAICR`CCGG,YG5169
ClaIAT`CG,AT5773SphIG,CATG`C5021
EcoNICCTNN`N,NNAGG4956SspIAAT|ATT191
EcoRIG`AATT,C5797Tth111IGACN`N,NGTC2142
FspAITGC|GCA2907XbaIT`CTAG,A5276
HindIIIA`AGCT,T5766XhoIC`TCGA,G5383
HpaIGTT|AAC3986ZraIGAC|GTC75
  
 

The recommended sequencing primer sequences for pET-His-MCS-Avi plasmid are as follows:
T7-R (5230-5248): 5'-CCCTATAGTGAGTCGTATTA-3'

Storage Conditions

Store at -20 °C.


Precautions

  • No commercial use of this plasmid is permitted without written permission from SBS Genetech. Nor shall it be transferred to any individual or institution outside the purchaser’s laboratory.
  • This product is intended solely for scientific‑research use by professionals. It shall not be adopted for clinical diagnosis or therapy, food‑related or pharmaceutical applications, nor kept in residential premises.
  • For your safety and health, wear a lab coat and disposable gloves during operation.

 

 

Only for research and not intended for treatment of humans or animals

 

 

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

 

 

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

 

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