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tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Products 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Enzymes
  • POCT 
    • Integrated POCT Platform
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Lateral Flow System
    • Freeze-Drying System
  • About 
    • About SBS
    • Solutions
    • Achievements
    • Legal Statement
  • Contact
  • …  
    • Home
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      • Catalog Products
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pET-Dual-His-SUMO3-Avi-MCS-BirA

pET-Dual-His-SUMO3-Avi-MCS-BirA

pET-Dual-His-SUMO3-Avi-MCS-BirA is a plasmid used to express biotin-tagged proteins in E. coli. This plasmid contains two sets of T7 promoter/lac operator and ribosome binding site (RBS). One set of multiple cloning sites (MCS) is preceded by His tag, SUMO3 tag and Avi tag, and the other set is used to express biotin ligase with S tag. BirA, therefore can efficiently initiate the co-expression of the target protein and BirA under the induction of isopropylthiogalactopyranoside (IPTG). In the presence of ATP and biotin (Biotin), the intracellularly expressed biotin ligase BirA catalyzes the covalent binding of biotin to the Avi tag at the N-terminus of the target protein, so that biotin labeling can be performed while expressing the target protein. At the same time, the plasmid contains a SUMO3 tag, which can be specifically recognized and cleaved by rSENP2 protease, thereby achieving efficient separation of the corresponding tagged protein and the target protein. This plasmid is ampicillin resistant.
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The main information of pET-Dual-His-SUMO3-Avi-MCS-BirA plasmid is as follows:
 

  • Feature Nucleotide
  • Position
  • T7-1 promoter
  • 6635-2
  • His tag
  • 77-94
  • SUMO3 tag
  • 101-376
  • Avi tag
  • 380-424
  • Multiple Cloning Sites
  • 430-468
  • T7-2 promoter
  • 533-551
  • BirA
  • 622-1584
  • S tag
  • 1597-1641
  • T7 Terminator
  • 1693-1740
  • f1 origin
  • 1777-2232
  • Ampicillin
  • 2347-3207
  • ori
  • 3381-3969
  • lacI
  • 5162-6244

 

The map of pET-Dual-His-SUMO3-Avi-MCS-BirA plasmid (6651bp) is as follows:

pET-Dual-His-SUMO3-Avi-MCS-BirA

 

Restriction sites not found in pET-Dual-His-SUMO3-Avi-MCS-BirA include:
 

AatIIAbsIAcc65IAcvIAgeIAhlIAleI
AsiGIAsiSIAsp718IAsuNHIBaeIBbrPIBcuI
BglIIBmtIBoxIBplIBshTIBsiWIBsp68I
BspOIBstPAIBstSNIBtuMICfr9ICfr42ICpoI
CsiICspICspACspCIEco32IEco72IEco105I
EcoRVEcoT22IFalIFseIIFspAII-CeuII-PpoI
I-SceIKflIKpnIKspIMabIMauBIMfeI
Mph1103IMreIMssIMunINheINruINsiI
OliIPfl23IIPI-PspIPI-SceIPinAIPmaCIPmeI
PmlIPshAIPspCIPspLIPsrIRgaIRigI
RruIRsrIIRsr2ISacIISanDISexAISfaAI
SfiISfr303ISgfISgrBISgrDISmaISmiI
SnaBISpeISrfISstIISwaITspMIXmaI
XmaCIZraIZsp2I  
   

The single enzyme cutting sites in pET-Dual-His-SUMO3-Avi-MCS-BirA include:
 

AflIIA`CRYG,T482NdeICA`TA,TG617
AhdIGACNN,N`NNGTC2420NotIGC`GGCC,GC469
AvrIIC`CTAG,G1664PacITTA,AT`TAA1660
BamHIG`GATC,C374PaeR7IC`TCGA,G1585
BbvCICC`TCA,GC1102PciIA`CATG,T4025
BmgBICAC|GTC352PflFIGACN`N,NGTC4283
BsmI 828PpuMIRG`GWC,CY5020
BspQIGCTCTTCN`NNN,4142PsiIA`CATG,T2135
BsrGIT`GTAC,A509PspXIVC`TCGA,GB1585
BstAPIGCAN,NNN`NTGC6213PvuICG,AT`CG2790
BstEIIG`GTNAC,C5707SacIG,AGCT`C441
BstZ17IGTA|TAC4258SalIG`TCGA,C456
Bsu36ICC`TNA,GG1567SapIGCTCTTCN`NNN,4142
DraIIICAC,NNN`GTG2010ScaIAGT|ACT2900
Eco53kIGAG|CTC439SgrAICR`CCGG,YG6570
EcoRIG`AATT,C431SphIG,CATG`C6422
FspITGC|GCA2642SspIAAT|ATT2215
HindIIIA`AGCT,T462StuIAGG|CCT233
HpaIGTT|AAC5387Tth111IGACN`N,NGTC4283
MluIA`CGCG,T5889XbaIT`CTAG,A30
NcoIC`CATG,G69XhoIC`TCGA,G1585


The recommended sequencing primer sequences for pET-Dual-His-SUMO3-Avi-MCS-BirA plasmid are as follows:
T7-R (533-552): 5'-CCCTATAGTGAGTCGTATTA-3'

Storage Conditions

Store at -20 °C.


Precautions

  • No commercial use of this plasmid is permitted without written permission from SBS Genetech. Nor shall it be transferred to any individual or institution outside the purchaser’s laboratory.
  • This product is intended solely for scientific‑research use by professionals. It shall not be adopted for clinical diagnosis or therapy, food‑related or pharmaceutical applications, nor kept in residential premises.
  • For your safety and health, wear a lab coat and disposable gloves during operation.

 

 

Only for research and not intended for treatment of humans or animals

 

 

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