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tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Products 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Enzymes
  • POCT 
    • Integrated POCT Platform
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Lateral Flow System
    • Freeze-Drying System
  • About 
    • About SBS
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    • Legal Statement
  • Contact
  • …  
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pET-Dual-His-MCS-Avi-BirA

pET-Dual-His-MCS-Avi-BirA

pET-Dual-His-MCS-Avi-BirA is a plasmid used to express biotin-tagged proteins in E. coli. This plasmid contains two sets of T7 promoter/lac operator and ribosome binding site (RBS). One set of multiple cloning sites (MCS) is located between His tag and Avi tag, and the other set is used to express biotin ligase with S tag. BirA, therefore can efficiently initiate the co-expression of the target protein and BirA under the induction of isopropylthiogalactopyranoside (IPTG). In the presence of ATP and biotin (Biotin), intracellular expression of BirA catalyzes the covalent binding of biotin to the Avi tag at the N-terminus of the target protein, so that biotin labeling can be performed while expressing the target protein. This plasmid is ampicillin resistant.
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The main information of pET-Dual-His-MCS-Avi-BirA plasmid is as follows:
 

  • Feature Nucleotide
  • Position
  • T7-1 promoter
  • 6364-2
  • His tag
  • 83-100
  • Multiple Cloning Sites
  • 101-160
  • Avi tag
  • 161-205
  • T7-2 promoter
  • 262-280
  • BirA
  • 351-1313
  • S tag
  • 1326-1370
  • T7 Terminator
  • 1422-1469
  • f1 origin
  • 1506-1961
  • Ampicillin
  • 2076-2936
  • ori
  • 3110-3698
  • lacI
  • 4891-5973

 

The map of pET-Dual-His-MCS-Avi-BirA plasmid (6380bp) is as follows:

pET-Dual-His-MCS-Avi-BirA

 

Restriction sites not found in pET-Dual-His-MCS-Avi-BirA include:
 

AatIAatIIAbsIAcc65IAcvIAgeIAhlI
AjiIAleIAsiGIAsiSIAsp718IAsuIIAsuNHI
BaeIBbrPIBcuIBglIIBmgBIBmtIBoxI
BplIBpu14IBshTIBsiWIBsp68IBsp119IBspOI
BspT104IBstBIBstPAIBstSNIBtrIBtuMICfr9I
Cfr42ICpoICspICsp45ICspAICspCIEco32I
Eco72IEco105IEco147IEcoRVEcoT22IFalIFseI
FspAII-CeuII-PpoII-SceIKpnIKspIMabI
MauBIMfeIMph1103IMreIMssIMunINheI
NruINsiINspVOliIPceIPfl23IIPI-PspI
PI-SceIPinAIPmaCIPmeIPmlIPshAIPspCI
PspLIPsrIRgaIRigIRsrIIRsr2ISacII
SanDISexAISfaAISfiISfr303ISfuISgfI
SgrBISgrDISmaISmiISnaBISpeISrfI
SseBISstIIStuISwaITspMIXmaIXmaCI
 

The single enzyme cutting sites in pET-Dual-His-MCS-Avi-BirA include:
 

AarICACCTGCNNNN`NNNN,1129NcoIC`CATG,G69
AflIIA`CRYG,T211NdeICA`TA,TG346
AhdIGACNN,N`NNGTC2149NotIGC`GGCC,GC150
AscIGG`CGCG,CC125PacITTA,AT`TAA1389
AvrIIC`CTAG,G1393PaeR7IC`TCGA,G1314
BamHIG`GATC,C106PasICC`CWG,GG434
BbvCICC`TCA,GC831PciIA`CATG,T3754
BlpIGC`TNA,GC1411PflFIGACN`N,NGTC4012
BsmIGAATG,CN`557PpuMIRG`GWC,CY4749
BspQIGCTCTTCN`NNN,3871PsiIA`CATG,T1864
BsrGIT`GTAC,A238PspXIVC`TCGA,GB1314
BstAPIGCAN,NNN`NTGC5942PvuICG,AT`CG2519
BstEIIG`GTNAC,C5436SacIG,AGCT`C122
BstZ17IGTA|TAC3987SalIG`TCGA,C137
Bsu36ICC`TNA,GG1296SapIGCTCTTCN`NNN,3871
DraIIICAC,NNN`GTG1739ScaIAGT|ACT2629
Eco53kIGAG|CTC120SgrAICR`CCGG,YG6299
EcoRIG`AATT,C112SphIG,CATG`C6151
FspITGC|GCA2371SspIAAT|ATT1944
HindIIIA`AGCT,T143Tth111IGACN`N,NGTC4012
HpaIGTT|AAC5116XbaIT`CTAG,A30
MluIA`CGCG,T5618XhoIC`TCGA,G1314
 
 

The recommended sequencing primer sequences for pET-Dual-His-MCS-Avi-BirA plasmid are as follows:
T7-R (262-281): 5'-CCCTATAGTGAGTCGTATTA-3'


Storage Conditions

Store at -20 °C.


Precautions

  • No commercial use of this plasmid is permitted without written permission from SBS Genetech. Nor shall it be transferred to any individual or institution outside the purchaser’s laboratory.
  • This product is intended solely for scientific‑research use by professionals. It shall not be adopted for clinical diagnosis or therapy, food‑related or pharmaceutical applications, nor kept in residential premises.
  • For your safety and health, wear a lab coat and disposable gloves during operation.

 

 

Only for research and not intended for treatment of humans or animals

 

 

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