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The main information of pET-Dual-His-Avi-MCS-BirA plasmid is as follows:
The map of pET-Dual-His-Avi-MCS-BirA plasmid (6378bp) is as follows:
Restriction sites not found in pET-Dual-His-Avi-MCS-BirA include:
| AarI | AbsI | AccIII | Acc36I | AccB7I | AcvI | AdeI | | AfeI | AgeI | AleI | AloI | Aor13HI | Aor51HI | AscI | | AsiGI | AsiSI | AspI | AspA2I | AvrII | AxyI | BaeI | | BalI | BarI | BbrPI | BbvCI | BfuAI | BlnI | BlpI | | Bpu1102I | BsaMI | Bse21I | BseAI | BshTI | BsmI | Bsp13I | | Bsp68I | Bsp1407I | Bsp1720I | BspEI | BspMI | BsrGI | BssNAI | | Bst1107I | BstAUI | BstENI | BstZ17I | Bsu36I | BtuMI | BveI | | CelII | Cfr42I | CpoI | CspI | CspAI | DraII | DraIII | | Eco47III | Eco72I | Eco81I | EcoNI | EcoO109I | FseI | FspAI | | I-CeuI | I-PpoI | I-SceI | KflI | Kpn2I | KspI | MauBI | | MlsI | MluNI | MreI | MroI | MroNI | MscI | Msp20I | | MssI | Mva1269I | NaeI | NgoMIV | NruI | OliI | PacI | | PalAI | PasI | PctI | PdiI | PflFI | PflMI | PfoI | | PI-PspI | PI-SceI | PinAI | PmaCI | PmeI | PmlI | PpuMI | | Psp5II | PspCI | PspPPI | PspXI | PsrI | PsyI | RgaI | | RigI | RruI | RsrII | Rsr2I | SacII | SanDI | SbfI | | SdaI | SfaAI | SfiI | Sfr303I | SgfI | SgrAI | SgrBI | | SgrDI | SgsI | SrfI | Sse8387I | SspBI | SstII | Tth111I | | Van91I | XagI | XmaJI | | |
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The single enzyme cutting sites in pET-Dual-His-Avi-MCS-BirA include:
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| AarI | CACCTGCNNNN`NNNN, | 1127 | NcoI | C`CATG,G | 69 | | AflII | A`CRYG,T | 209 | NdeI | CA`TA,TG | 344 | | AhdI | GACNN,N`NNGTC | 2147 | NotI | GC`GGCC,GC | 196 | | AscI | GG`CGCG,CC | 171 | PacI | TTA,AT`TAA | 1387 | | AvrII | C`CTAG,G | 1391 | PaeR7I | C`TCGA,G | 1312 | | BamHI | G`GATC,C | 152 | PasI | CC`CWG,GG | 432 | | BbvCI | CC`TCA,GC | 829 | PciI | A`CATG,T | 3752 | | BlpI | GC`TNA,GC | 1409 | PflFI | GACN`N,NGTC | 4010 | | BsmI | GAATG,CN` | 555 | PpuMI | RG`GWC,CY | 4747 | | BspQI | GCTCTTCN`NNN, | 3869 | PsiI | A`CATG,T | 1862 | | BsrGI | T`GTAC,A | 236 | PspXI | VC`TCGA,GB | 1312 | | BstAPI | GCAN,NNN`NTGC | 5940 | PvuI | CG,AT`CG | 2517 | | BstEII | G`GTNAC,C | 5434 | SacI | G,AGCT`C | 168 | | BstZ17I | GTA|TAC | 3985 | SalI | G`TCGA,C | 183 | | Bsu36I | CC`TNA,GG | 1294 | SapI | GCTCTTCN`NNN, | 3869 | | DraIII | CAC,NNN`GTG | 1737 | ScaI | AGT|ACT | 2627 | | Eco53kI | GAG|CTC | 166 | SgrAI | CR`CCGG,YG | 6297 | | EcoRI | G`AATT,C | 158 | SphI | G,CATG`C | 9149 | | FspI | TGC|GCA | 2369 | SspI | AAT|ATT | 1942 | | HindIII | A`AGCT,T | 189 | Tth111I | GACN`N,NGTC | 4010 | | HpaI | GTT|AAC | 5114 | XbaI | T`CTAG,A | 30 | | MluI | A`CGCG,T | 5616 | XhoI | C`TCGA,G | 1312 |
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The recommended sequencing primer sequences for pET-Dual-His-Avi-MCS-BirA plasmid are as follows:
T7-R (259-279): 5'-CCCTATAGTGAGTCGTATTA-3'
Storage Conditions
Store at -20 °C.
Precautions
- No commercial use of this plasmid is permitted without written permission from SBS Genetech. Nor shall it be transferred to any individual or institution outside the purchaser’s laboratory.
- This product is intended solely for scientific‑research use by professionals. It shall not be adopted for clinical diagnosis or therapy, food‑related or pharmaceutical applications, nor kept in residential premises.
- For your safety and health, wear a lab coat and disposable gloves during operation.
Only for research and not intended for treatment of humans or animals
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