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tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Products 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Enzymes
  • POCT 
    • Integrated POCT Platform
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Lateral Flow System
    • Freeze-Drying System
  • About 
    • About SBS
    • Solutions
    • Achievements
    • Legal Statement
  • Contact
  • …  
    • Home
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      • All Products
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      • Catalog Products
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pCMV-C-mOrange2

pCMV-C-mOrange2

pCMV-C-mOrange2 is a mammalian cell expression plasmid used to express fusion proteins containing mOrange2 tags at the C terminus. mOrange2 protein is an extremely bright orange fluorescent protein with improved protein stability compared to mOrange protein.
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All products have special prices for bulk purchase, please contact for more details if required.

 

 

The main information of pCMV-C-mOrange2 plasmid is as follows:

  • Feature Nucleotide
  • Position
  • CMV promoter
  • 1-602
  • T3 promoter and T3 primer binding site
  • 620-639
  • Multiple cloning site
  • 651-740
  • mOrange2
  • 741-1448
  • T7 promoter and T7 primer binding site
  • 1498-1519
  • SV40 polyA signal
  • 1531-1914
  • f1 origin of ss-DNA replication
  • 2052-2356
  • bla promoter
  • 2381-2505
  • SV40 promoter
  • 2525-2863
  • Neomycin/kanamycin resistance ORF
  • 2898-3689
  • HSV-thymidine kinase (TK) polyA signal
  • 3690-4148
  • pUC origin
  • 4277-4944

 

 

The map of pCMV-C-mOrange2 plasmid (4996bp) is as follows:

pCMV-C-mOrange2

 

Restriction enzymes that do not cut pCMV-C-mOrange2 include:

AclIAfeIAgeIAhdIAscIAsiSIBbsI
BcgIBlpIBmgBIBpu10IBsiWIBsmBIBspEI
BsrGIBssHIIBstZ17IEarIEcoNIEsp3IFseI
PmeIPmlIPpuMIPshAIPspXISapISbfI
SgrAISpeISwaIXcmI 
   

The single enzyme cutting sites (Restriction enzymes that cut pCMV-C-mOrange2 once) in pCMV-C-mOrange2 include:

AccIGT`MK,AC718MluIA`CGCG,T1914
AflIIC`TTAA,G1251MscITGG|CCA3108
AleICACNN|NNGTG661NarIGG`CG,CC3026
ApaIG,GGCC`C1453NheIG`CTAG,C597
ApaLIG`TGCA,C4630NotIGC`GGCC,GC668
BaeI,N)5`(N)10ACNNNNGTAYC(N)7,(N)5`1185PaeR7IC`TCGA,G729
BamHIG`GATC,C687PciIA`CATGT4944
BclIT`GATC,A1685PflFICCAN,NNN`NTGG3144
BglIIA`GATC,T723PflMIG,GCGC`C1146
BmtIG,CTAG`C601PluTIG,GCGC`C3029
BsaIGGTCTCN`NNNN,4015PspOMIG`GGCC,C1449
BsaXI,NNN`(N)9AC(N)5CTCC(N)7,NNN`2078PstIC`TGCA,G703
BseRIGAGGAG(N)8,NN`2844PvuICG,AT`CG1531
BsgIGTGCAG(N)14,NN`1420RsrIICG`GWC,CG3524
BspDIAT`CG,AT2866SacIG,AGCT`C655
BstBITT`CG,AA3708SacIICC,GC`GG662
BstEIIG`GTNAC,C893SalIG`TCGA,C717
BstXICCAN,NNNN`NTGG663SfiIGGCCN,NNN`NGGCC2801
BtsIGCAGTG,NN`1867SfoIGGC|GCC3027
ClaIAT`CG,AT2866SmaICCC|GGG682
CspCI,NN`(N)11CAA(N)5GTGG(N)10,NN`382SnaBITAC|GTA346
DraIIICAC,NNN`GTG2144SrfIGCCC|GGGC682
Eco53kIGAG|CTC653StuIAGG|CCT2847
EcoRIG`AATT,C705TspMIC`CCGG,G680
EcoRVGAT|ATC713Tth111IGACN`N,NGTC3144
HindIIIA`AGCT,T693XbaIT`CTAG,A735
HpaICC,TGCA`GG1791XhoIC`TCGA,G729
KasIG`GCGC,C3025XmaIC`CCGG,G680
MfeIC`AATT,G1778XmnIGAANN|NNTTC871
 

 

The recommended sequencing primer sequences for pCMV-C-mOrange2 plasmid are as follows:

T3 primer (620-639): 5'-AATTAACCCTCACTAAAGGG-3'

C-mOrange2 primer (852-873): 5'- AACCTTCGTATGGACGACCTTC -3'

 

Storage Conditions

Store at -20 °C.


Precautions

  • No commercial use of this plasmid is permitted without written permission from SBS Genetech. Nor shall it be transferred to any individual or institution outside the purchaser’s laboratory.
  • This product is intended solely for scientific‑research use by professionals. It shall not be adopted for clinical diagnosis or therapy, food‑related or pharmaceutical applications, nor kept in residential premises.
  • For your safety and health, wear a lab coat and disposable gloves during operation.

 

 

Only for research and not intended for treatment of humans or animals

 

 

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