thumbnail image
tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Products 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Enzymes
  • POCT 
    • Integrated POCT Platform
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Lateral Flow System
    • Freeze-Drying System
  • About 
    • About SBS
    • Solutions
    • Achievements
    • Legal Statement
  • Contact
  • …  
    • Home
    • Products 
      • All Products
      • Custom Services
      • Catalog Products
      • Innovative Systems
      • Nucleic Acid Related
      • Enzymes
    • POCT 
      • Integrated POCT Platform
      • LAMP
      • RPA
      • CRISPR
      • DNA-Free Enzymes
      • Lateral Flow System
      • Freeze-Drying System
    • About 
      • About SBS
      • Solutions
      • Achievements
      • Legal Statement
    • Contact
    • Login
tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Products 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Enzymes
  • POCT 
    • Integrated POCT Platform
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Lateral Flow System
    • Freeze-Drying System
  • About 
    • About SBS
    • Solutions
    • Achievements
    • Legal Statement
  • Contact
  • …  
    • Home
    • Products 
      • All Products
      • Custom Services
      • Catalog Products
      • Innovative Systems
      • Nucleic Acid Related
      • Enzymes
    • POCT 
      • Integrated POCT Platform
      • LAMP
      • RPA
      • CRISPR
      • DNA-Free Enzymes
      • Lateral Flow System
      • Freeze-Drying System
    • About 
      • About SBS
      • Solutions
      • Achievements
      • Legal Statement
    • Contact
    • Login
Beijing SBS Genetech Co.,Ltd.
Go Back
pCMV-C-mCherry

pCMV-C-mCherry

pCMV-C-mCherry is a mammalian cell expression plasmid used to express fusion proteins containing mCherry (a mutant of DsRed, a very bright red fluorescent protein) tag at the C terminus. This plasmid contains the CMV promoter, which can efficiently promote the expression of the target protein in cells. There is a complete coding sequence of mCherry behind the multiple cloning site, so the fusion protein containing the mCherry tag at the C terminus can be expressed by inserting the target gene into the multiple cloning site according to the reading frame. The fluorescent properties of mCherry can be used to easily observe the expression level and intracellular localization of the fusion protein, and mCherry antibodies can also be used to detect or immunoprecipitate the fusion protein.
Get a Quote
More Details

All products have special prices for bulk purchase, please contact for more details if required.

 

 

The main information of pCMV-C-mCherry plasmid is as follows:

  • Feature Nucleotide
  • Position
  • CMV promoter
  • 1-602
  • T3 promoter and T3 primer binding site
  • 620-639
  • Multiple cloning site
  • 651-736
  • mCherry
  • 741-1451
  • T7 promoter and T7 primer binding site
  • 1501-1522
  • SV40 polyAsignal
  • 1534-1917
  • f1 origin of ss-DNA replication
  • 2055-2361
  • bla promoter
  • 2386-2510
  • SV40 promoter
  • 2530-2868
  • Neomycin/kanamycin resistance ORF
  • 2903-3694
  • HSV-thymidine kinase (TK) polyA signal
  • 3695-4153
  • pUC origin
  • 4282-4949

 

The map of pCMV-C-mCherry plasmid (5001bp) is as follows:

pCMV-C-mCherry

 

Restriction enzymes that do not cut pCMV-C-mCherry include:

AclIAfeIAflIIAgeIAscIAsiSIBaeI
BcgIBlpIBmgBIBsiWIBsmBIBspEIBspQI
BssHIIBstZ17IEarIEcoNIFseINruIPmeI
PmlIPpuMIPshAIPspXISapIScaISpeI
SwaIXmnI

The single restriction enzyme cutting sites (Restriction enzymes that cut pCMV-C-mCherry once) in pCMV-C-mCherry include:

NdeICA`TA,TG240 PflMICCAN,NNN`NTGG1177
SnaBITAC|GTA346 Bpu10ICC`TNA,GC1282
CspCI,NN`(N)11CAA(N)5GTGG(N)10,NN`380 BbvCICC`TCA,GC1282
NheIG`CTAG,C597 BsgIGTGCAG(N)14,NN`1333
BmtIG,CTAG`C597 SgrAICR`CCGG,YG1422
SacIG,AGCT`C651 XcmICCANNNN,N`NNNNTGG1426
Eco53kIGAG|CTC653 BsrGIT`GTAC,A1441
BstXICCAN,NNNN`NTGG659 PspOMIG`GGCC,C1452
SacIICC,GC`GG660 ApaIG,GGCC`C1452
NotIGC`GGCC,GC668 PvuICG,AT`CG1532
TspMIC`CCGG,G680 BclIT`GATC,A1688
XmaIC`CCGG,G680 MfeIC`AATT,G1781
SmaICCC|GGG682 HpaIGTT|AAC1794
BamHIG`GATC,C687 BtsIGCAGTG,NN`1868
HindIIIA`AGCT,T693 MluIA`CGCG,T1917
EcoRIG`AATT,C705 DraIIICAC,NNN`GTG2144
EcoRVGAT|ATC713 SfiIGGCCN,NNN`NGGCC2803
SalIG`TCGA,C717 ClaIAT`CG,AT2871
AccIGT`MK,AC718 BspDIAT`CG,AT2871
BglIIA`GATC,T723 PflFIGACN`N,NGTC3149
XhoIC`TCGA,G729 Tth111IGACN`N,NGTC3149
PaeR7IC`TCGA,G729 BsrDIGCAATG,NN`3262
XbaIT`CTAG,A735 RsrIICG`GWC,CG3547
AhdIGACNN,N`NNGTC934 BstBITT`CG,AA3713
SbfICC,TGCA`GG1092 BsaIGGTCTCN`NNNN,4020
BbsIGAAGACNN`NNNN,1176 PciIA`CATG,T4949
 

 

The recommended sequencing primer sequences for pCMV-C-mCherry plasmid are as follows:
T3 primer (620-639): 5′-AATTAACCCTCACTAAAGGG-3′
C-mCherry primer (852-873): 5′CCTCGTAGGGGCGGCCCTCGCC-3′

Storage Conditions

Store at -20 °C.


Precautions

  • No commercial use of this plasmid is permitted without written permission from SBS Genetech. Nor shall it be transferred to any individual or institution outside the purchaser’s laboratory.
  • This product is intended solely for scientific‑research use by professionals. It shall not be adopted for clinical diagnosis or therapy, food‑related or pharmaceutical applications, nor kept in residential premises.
  • For your safety and health, wear a lab coat and disposable gloves during operation.

 

 

Only for research and not intended for treatment of humans or animals

 

 

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

 

 

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

 

  • Precision Molecular Technologies

    from Research to Commercial Scale

    For over 25 years, SBS Genetech has delivered

    high-quality, rigorously validated molecular reagents

    trusted by leading research institutions and

    biotechnology companies across 60+ countries.

    Explore Products
    OEM & Custom Solutions
  • Scientific Validation Behind Our Technologies

    We are honored to create value for our customers and facilitate the development of science

SBS Genetech © Copyright 2000-2026

from China, for the World

for Superior Biology Services since 2000

    Home
    Journals
    Contact
    Posts
Cookie Use
We use cookies to ensure a smooth browsing experience. By continuing we assume you accept the use of cookies.
Learn More