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tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Products 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Enzymes
  • POCT 
    • Integrated POCT Platform
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    • CRISPR
    • DNA-Free Enzymes
    • Lateral Flow System
    • Freeze-Drying System
  • About 
    • About SBS
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pCMV-C-DsRed

pCMV-C-DsRed

pCMV-C-DsRed is a mammalian cell expression plasmid used to express fusion proteins containing a DsRed (Discosoma sp. red fluorescent protein) tag at the C terminus. This plasmid contains the CMV promoter, which can efficiently promote the expression of the target protein in cells. There is a complete coding sequence of DsRed behind the multiple cloning site, so the fusion protein containing a DsRed tag at the C terminus can be expressed by inserting the target gene into the multiple cloning site according to the reading frame. The fluorescence properties of DsRed can be used to easily observe the expression level and intracellular localization of the fusion protein, and DsRed antibodies can also be used to detect or immunoprecipitate the fusion protein. DsRed has no sequence homology with GFP, so DsRed cannot be detected using GFP antibodies. This plasmid is kanamycin resistant. After transfecting cells, G418 can be used to select cell lines that stably express the target protein.
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The main information of pCMV-C-DsRed plasmid is as follows:

  • Feature Nucleotide
  • Position
  • CMV promoter
  • 1-602
  • T3 promoter and T3 primer binding site
  • 620-639
  • Multiple cloning site
  • 651-740
  • DsRed
  • 741-1419
  • T7 promoter and T7 primer binding site
  • 1478-1499
  • SV40 polyA signal
  • 1511-1894
  • f1 origin of ss-DNA replication
  • 2032-2338
  • bla promoter
  • 2363-2487
  • SV40 promoter
  • 2507-2845
  • Neomycin/kanamycin resistance ORF
  • 2869-3661
  • HSV-thymidine kinase (TK) polyA signal
  • 3662-4130
  • pUC origin
  • 4259-4926

 

The map of pCMV-C-DsRed plasmid (4969bp) is as follows:

pCMV-C-DsRed

 

Restriction enzymes that do not cut pCMV-C-DsRed include:

AclIAflIIAgeIAscIAsiSIBaeIBbvCI
BcgIBlpIBmgBIBpu10IBsiWIBsmBIBspEI
BspQIBssHIIBstZ17IEarIEcoNIFseINruI
PmeIPmlIPpuMIPshAIPspXISapIScaI
SgrAISpeISwaIXcmIXmnI
   

The single restriction enzymes (Restriction enzymes that cut pCMV-C-DsRed once) in pCMV-C-DsRed include:

NdeICA`TA,TG240 BbsIGAAGACNN`NNNN,1161
SnaBITAC|GTA346 BsrGIT`GTAC,A1279
CspCI,NN`(N)11CAA(N)5GTGG(N)10,NN`380 PflMICCAN,NNN`NTGG1319
NheIG`CTAG,C597 PspOMIG`GGCC,C1420
BmtIG,CTAG`C597 ApaIG,GGCC`C1420
SacIG,AGCT`C651 PvuIGCAGTG,NN`1500
Eco53kIGAG|CTC653 BclIT`GATC,A1656
BstXICCAN,NNNN`NTGG659 MfeIC`AATT,G1749
SacIICC,GC`GG660 HpaIGTT|AAC1762
NotIGC`GGCC,GC668 BtsIGCAGTG,NN`1836
TspMIC`CCGG,G680 MluIA`CGCG,T1885
XmaIC`CCGG,G680 SfiIGGCCN,NNN`NGGCC2771
SmaICCC|GGG682 ClaIAT`CG,AT2839
SrfIGCCC|GGGC682 BspDIAT`CG,AT2839
BamHIG`GATC,C687 KasIG`GCGC,C2998
HindIIIA`AGCT,T693 NarIGG`CG,CC2999
EcoRIG`AATT,C705 PluTIG,GCGC`C2998
EcoRVGAT|ATC713 SfoIGGC|GCC3000
SalIG`TCGA,C717 MscITGG|CCA3081
AccIGT`MK,AC718 Tth111IGACN`N,NGTC3117
XhoIC`TCGA,G729 PflFIGACN`N,NGTC3117
PaeR7IC`TCGA,G729 BsrDIGCAATG,NN`3230
XbaIT`CTAG,A735 RsrIICG`GWC,CG3515
BstEIIG`GTNAC,C881 BstBITT`CG,AA3681
AhdIGACNN,N`NNGTC919 BsaIGGTCTCN`NNNN,3988
AfeIAGC|GCT1023 ApaLIG`TGCA,C4603
SbfICC,TGCA`GG1077 PciIA`CATG,T4917


The recommended sequencing primer sequences for pCMV-C-DsRed plasmid are as follows:
T3 primer (620-639): 5′-AATTAACCCTCACTAAAGGG-3′
C-DsRed primer (852-873): 5′- TGGCGGTCTGGGTGCCCTCGTA -3′
 

Storage Conditions

Store at -20 °C.


Precautions

  • No commercial use of this plasmid is permitted without written permission from SBS Genetech. Nor shall it be transferred to any individual or institution outside the purchaser’s laboratory.
  • This product is intended solely for scientific‑research use by professionals. It shall not be adopted for clinical diagnosis or therapy, food‑related or pharmaceutical applications, nor kept in residential premises.
  • For your safety and health, wear a lab coat and disposable gloves during operation.

 

 

Only for research and not intended for treatment of humans or animals

 

 

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