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tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Products 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Enzymes
  • POCT 
    • Integrated POCT Platform
    • LAMP
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    • CRISPR
    • DNA-Free Enzymes
    • Lateral Flow System
    • Freeze-Drying System
  • About 
    • About SBS
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  • Contact
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pCMV-C-CFP

pCMV-C-CFP

pCMV-C-CFP is a mammalian cell expression plasmid used to express fusion proteins containing a CFP (Cyan Fluorescent Protein, cyan fluorescent protein) tag at the C terminus. This plasmid contains the CMV promoter, which can efficiently promote the expression of the target protein in cells. There is a complete coding sequence of CFP behind the multiple cloning site, so the fusion protein containing a CFP tag at the C terminus can be expressed by inserting the target gene according to the reading frame at the multiple cloning site. The fluorescence properties of CFP can be used to easily observe the expression level and intracellular localization of the fusion protein, and CFP antibodies can also be used to detect or immunoprecipitate the fusion protein. CFP is highly similar to GFP, so you can try to use GFP antibodies to detect CFP. This plasmid is kanamycin resistant. After transfecting cells, G418 can be used to select cell lines that stably express the target protein.
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The main information of pCMV-C-CFP plasmid is as follows:

  • Feature Nucleotide
  • Position
  • CMV promoter
  • 1-602
  • T3 promoter and T3 primer binding site
  • 620-639
  • Multiple cloning site
  • 651-740
  • CFP
  • 741-1460
  • T7 promoter and T7 primer binding site
  • 1510-1531
  • SV40 polyA signal
  • 1543-1926
  • f1 origin of ss-DNA replication
  • 2064-2370
  • bla promoter
  • 2395-2519
  • SV40 promoter
  • 2539-2877
  • Neomycin/kanamycin resistance ORF
  • 2912-3703
  • HSV-thymidine kinase (TK) polyA signal
  • 3704-4162
  • pUC origin
  • 4291-4958

 

The map of pCMV-C-CFP plasmid (5010bp) is as follows:

pCMV-C-CFP

 

Restriction enzymes that do not cut pCMV-C-CFP include:

AclIAfeIAflIIAgeIAhdIAscIAsiSI
BaeIBbsIBbvCIBlpIBmgBIBsiWIBsmBI
BspEIBspQIBssHIIBstEIIBstZ17IEarIEcoNI
FseINruIPflMIPmeIPmlIPpuMIPshAI
PspXISapISbfIScaISgrAISpeISwaI
XcmIXmnI
   

The single restriction enzymes (Restriction enzymes that cut pCMV-C-CFP once) in pCMV-C-CFP include:

NdeICA`TA,TG240PspOMIG`GGCC,C1461
SnaBITAC|GTA346ApaIIG,GGCC`C1461
CspCI,NN`(N)11CAA(N)5GTGG(N)10,NN`380PvuICG,AT`CG1541
NheIG`CTAG,C597BclIT`GATC,A1697
BmtIG,CTAG`C597MfeIC`AATT,G1790
SacIG,AGCT`C651HpaIGTT|AAC1803
Eco53kIGAG|CTC653MluIA`CGCG,T1926
BstXICCAN,NNNN`NTGG659BsaXI,NNN`(N)9AC(N)5CTCC(N)7,NNN`2087
SacIICC,GC`GG660DraIIICAC,NNN`GTG2153
NotIGC`GGCC,GC668SfiIGGCCN,NNN`NGGCC2812
TspMIC`CCGG,G680StuIAGG|CCT2861
XmaIC`CCGG,G680ClaIAT`CG,AT2880
SmaICCC|GGG682BspDIAT`CG,AT2880
BamHIG`GATC,C687KasIG`GCGC,C3039
HindIIIA`AGCT,T693NarIGG`CG,CC3040
PstIC,TGCA`G699SfoIGGC|GCC3041
EcoRIG`AATT,C705PluTIG,GCGC`C3039
EcoRVGAT|ATC713MscITGG|CCA3122
SalIG`TCGA,C717Tth111IGACN`N,NGTC3158
AccIGT`MK,AC718PflFIGACN`N,NGTC3158
BglIIA`GATC,T723BsrDIGCAATG,NN`3271
XhoIC`TCGA,G729RsrIICG`GWC,CG3556
PaeR7IC`TCGA,G729BstBITT`CG,AA3722
XbaIT`CTAG,A735BsaIGGTCTCN`NNNN,4029
BcgI,NN`(N)10CGA(N)6TGC(N)10,NN`829ApaLIG`TGCA,C4644
BsrGIT`GTAC,A1450PciIA`CATG,T4958

 

The recommended sequencing primer sequences for pCMV-C-CFP plasmid are as follows:
T3 primer (620-639): 5′-AATTAACCCTCACTAAAGGG-3′
C-CFP primer (852-873): 5′- GGGTCAGCTTGCCGTAGGTGGC -3′
 

Storage Conditions

Store at -20 °C.


Precautions

  • No commercial use of this plasmid is permitted without written permission from SBS Genetech. Nor shall it be transferred to any individual or institution outside the purchaser’s laboratory.
  • This product is intended solely for scientific‑research use by professionals. It shall not be adopted for clinical diagnosis or therapy, food‑related or pharmaceutical applications, nor kept in residential premises.
  • For your safety and health, wear a lab coat and disposable gloves during operation.

 

 

Only for research and not intended for treatment of humans or animals

 

 

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