thumbnail image
tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Products 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Enzymes
  • POCT 
    • Integrated POCT Platform
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Lateral Flow System
    • Freeze-Drying System
  • About 
    • About SBS
    • Solutions
    • Achievements
    • Legal Statement
  • Contact
  • …  
    • Home
    • Products 
      • All Products
      • Custom Services
      • Catalog Products
      • Innovative Systems
      • Nucleic Acid Related
      • Enzymes
    • POCT 
      • Integrated POCT Platform
      • LAMP
      • RPA
      • CRISPR
      • DNA-Free Enzymes
      • Lateral Flow System
      • Freeze-Drying System
    • About 
      • About SBS
      • Solutions
      • Achievements
      • Legal Statement
    • Contact
    • Login
tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Products 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Enzymes
  • POCT 
    • Integrated POCT Platform
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Lateral Flow System
    • Freeze-Drying System
  • About 
    • About SBS
    • Solutions
    • Achievements
    • Legal Statement
  • Contact
  • …  
    • Home
    • Products 
      • All Products
      • Custom Services
      • Catalog Products
      • Innovative Systems
      • Nucleic Acid Related
      • Enzymes
    • POCT 
      • Integrated POCT Platform
      • LAMP
      • RPA
      • CRISPR
      • DNA-Free Enzymes
      • Lateral Flow System
      • Freeze-Drying System
    • About 
      • About SBS
      • Solutions
      • Achievements
      • Legal Statement
    • Contact
    • Login
Beijing SBS Genetech Co.,Ltd.
Go Back
pCMV-C-BFP

pCMV-C-BFP

pCMV-C-BFP is a mammalian cell expression plasmid independently developed by Beyuntian. It is used to express fusion proteins containing a BFP (Blue Fluorescent Protein, blue fluorescent protein) tag at the C terminus. This plasmid contains the CMV promoter, which can efficiently promote the expression of the target protein in cells. There is a complete coding sequence of BFP behind the multiple cloning site, so the fusion protein containing a BFP tag at the C terminus can be expressed by inserting the target gene according to the reading frame at the multiple cloning site. The fluorescence properties of BFP can be used to easily observe the expression level and intracellular localization of the fusion protein, and BFP antibodies can also be used to detect or immunoprecipitate the fusion protein. BFP is highly similar to GFP, so you can try to detect BFP with GFP antibodies. This plasmid is kanamycin resistant.
Get a Quote
More Details

All products have special prices for bulk purchase, please contact for more details if required.

 

 

The main information of pCMV-C-BFP plasmid is as follows:

  • Feature Nucleotide
  • Position
  • CMV promoter
  • 1-602
  • T3 promoter and T3 primer binding site
  • 620-639
  • Multiple cloning site
  • 651-740
  • BFP
  • 741-1460
  • T7 promoter and T7 primer binding site
  • 1510-1531
  • SV40 polyA signal
  • 1543-1926
  • f1 origin of ss-DNA replication
  • 2064-2370
  • bla promoter
  • 2395-2519
  • SV40 promoter
  • 2539-2877
  • Neomycin/kanamycin resistance ORF
  • 2912-3703
  • HSV-thymidine kinase (TK) polyA signal
  • 3704-4162
  • pUC origin
  • 4291-4958

 

The map of pCMV-C-BFP plasmid (5010bp) is as follows:

pCMV-C-BFP

 

Restriction enzymes that do not cut pCMV-C-BFP include:

AclIAfeIAflIIAgeIAhdIAscIAsiSI
BaeIBbsIBbvCIBlpIBmgBIBsiWIBsmBI
BspEIBspQIBssHIIBstEIIBstZ17IEarIEcoNI
FseINruIPflMIPmeIPmlIPpuMIPshAI
PspXISapISbf IScaISgrAISpeISwaI
XcmIXmnI
   

The single restriction enzymes (Restriction enzymes that cut pCMV-C-BFP once) in pCMV-C-BFP include:

NdeICA`TA,TG240 ApaIIG,GGCC`C1461
SnaBITAC|GTA346 PvuICG,AT`CG1541
CspCI,NN`(N)11CAA(N)5GTGG(N)10,NN`380 BclIT`GATC,A1697
NheIG`CTAG,C597 MfeIC`AATT,G1790
BmtIG,CTAG`C597 HpaIGTT|AAC1803
SacIG,AGCT`C651 BtsIGCAGTG,NN`1877
Eco53kIGAG|CTC653 MluIA`CGCG,T1926
BstXICCAN,NNNN`NTGG659 BsaXI,NNN`(N)9AC(N)5CTCC(N)7,NNN`2087
SacIICC,GC`GG660 DraIIICAC,NNN`GTG2153
NotIGC`GGCC,GC668 SfiIGGCCN,NNN`NGGCC2812
TspMIC`CCGG,G680 StuIAGG|CCT2861
XmaIC`CCGG,G680 ClaIAT`CG,AT2880
SmaICCC|GGG682 BspDIAT`CG,AT2880
BamHIG`GATC,C687 KasIG`GCGC,C3039
HindIIIA`AGCT,T693 NarIGG`CG,CC3040
PstIC,TGCA`G699 SfoIGGC|GCC3041
EcoRIG`AATT,C705 PluTIG,GCGC`C3039
EcoRVGAT|ATC713 MscITGG|CCA3122
SalIG`TCGA,C717 Tth111IGACN`N,NGTC3158
AccIGT`MK,AC718 PflFIGACN`N,NGTC3158
BglIIA`GATC,T723 BsrDIGCAATG,NN`3271
XhoIC`TCGA,G729 RsrIICG`GWC,CG3556
PaeR7IC`TCGA,G729 BstBITT`CG,AA3722
XbaIT`CTAG,A735 BsaIGGTCTCN`NNNN,4029
BcgI,NN`(N)10CGA(N)6TGC(N)10,NN`829 ApaLIG`TGCA,C4644
BsrGIT`GTAC,A1450 PciIA`CATG,T4958
PspOMIG`GGCC,C1461 



The recommended sequencing primer sequences for pCMV-C-BFP plasmid are as follows:
T3 primer (620-639): 5′-AATTAACCCTCACTAAAGGG-3′
C-BFP primer (852-873): 5′- GGGTCAGCTTGCCGTAGGTGGC -3′


Storage Conditions

Store at -20 °C.


Precautions

  • No commercial use of this plasmid is permitted without written permission from SBS Genetech. Nor shall it be transferred to any individual or institution outside the purchaser’s laboratory.
  • This product is intended solely for scientific‑research use by professionals. It shall not be adopted for clinical diagnosis or therapy, food‑related or pharmaceutical applications, nor kept in residential premises.
  • For your safety and health, wear a lab coat and disposable gloves during operation.

 

 

Only for research and not intended for treatment of humans or animals

 

 

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

 

 

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

 

  • Precision Molecular Technologies

    from Research to Commercial Scale

    For over 25 years, SBS Genetech has delivered

    high-quality, rigorously validated molecular reagents

    trusted by leading research institutions and

    biotechnology companies across 60+ countries.

    Explore Products
    OEM & Custom Solutions
  • Scientific Validation Behind Our Technologies

    We are honored to create value for our customers and facilitate the development of science

SBS Genetech © Copyright 2000-2026

from China, for the World

for Superior Biology Services since 2000

    Home
    Journals
    Contact
    Posts
Cookie Use
We use cookies to ensure a smooth browsing experience. By continuing we assume you accept the use of cookies.
Learn More