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tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Products 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Enzymes
  • POCT 
    • Integrated POCT Platform
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Lateral Flow System
    • Freeze-Drying System
  • About 
    • About SBS
    • Solutions
    • Achievements
    • Legal Statement
  • Contact
  • …  
    • Home
    • Products 
      • All Products
      • Custom Services
      • Catalog Products
      • Innovative Systems
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    • POCT 
      • Integrated POCT Platform
      • LAMP
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      • DNA-Free Enzymes
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pCMV‑Blank

pCMV‑Blank

pCMV‑Blank is an expression plasmid designed for target‑protein expression in mammalian cells. It carries the CMV promoter, which enables high‑efficiency transcription of the target protein inside host cells. This plasmid confers kanamycin resistance. Following cell transfection, G418 can be used to screen for cell lines with stable target‑protein expression.
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The main information of pCMV-Blank plasmid is as follows:

  • Feature Nucleotide
  • Position
  • CMV promoter
  • 1-602
  • T3 promoter and T3 primer binding site
  • 620-639
  • multiple cloning site
  • 680-753
  • T7 promoter and T7 primer binding site
  • 796-817
  • SV40 polyA signal
  • 829-1212
  • f1 origin of ss-DNA replication
  • 1350-1654
  • bla promoter
  • 1679-1803
  • SV40 promoter
  • 1823-2161
  • neomycin/kanamycin resistance ORF
  • 2196-2987
  • HSV-thymidine kinase (TK) polyA signal
  • 2988-3446
  • pUC origin
  • 3575-4242

 

The map of pCMV-Blank plasmid is as follows:

pET-Dual-His-SUMO3-Avi-MCS-BirA

 

Restriction sites not found in pCMV-Blank include:

Afl IIAge IAhd IAsc IBbs IBbv IIBlp I
Bsg IBsiW IBsmB IBspM IIBsrG IBssH IIBst1107 I
BstE IIEar IEco47 IIIEco72 IEcoN IEsp IFse I
Nru IPflM IPme IPml IPpuM IPsp1406 ISap I
Sca ISpl I
   

The single enzyme cutting sites in pCMV-Blank​ include:

Nde ICA`TA,TG241 Pvu ICG,AT`CG830
SnaB ITAC|GTA347 Bcl IT`GATC,A984
Nhe IG`CTAG,C598 Mun IC`AATT,G1077
Sac IG,AGCT`C656 Hpa IGTT|AAC1090
Sac IICC,GC`GG663 Mlu IA`CGCG,T1213
BstX ICCAN,NNNN`NTGG664 Dra IIICAC,NNN`GTG1443
Not IGC`GGCC,GC669 Sfi IGGCCN,NNN`NGGCC2100
PspA IC`CCGG,G681 BseR IGAGGAG 16/142143
Xma IC`CCGG,G681 Stu IAGG|CCT2146
Srf IGCCC|GGGC683 Cla IAT`CG,AT2165
Sma ICCC|GGG683 Kas IG`GCGC,C2324
BamH IG`GATC,C688 Nar IGG`CG,CC2325
Hind IIIA`AGCT,T694 Ehe IGGC|GCC2326
Pst IC,TGCA`G704 Bbe IG,GCGC`C2328
EcoR IG`AATT,C706 Msc ITGG|CCA2407
EcoR VGAT|ATC714 Tth111 IGACN`N,NGTC2443
Sal IG`TCGA,C718 BsrD IGCAATG, 82558
Acc IGT`MK,AC719 Bsp1286 IG,DGCH`C2628
Bgl IIA`GATC,T724 Rsr IICG`GWC,CG2841
PaeR7 IC`TCGA,G730 BsiC ITT`CG,AA3007
Xho IC`TCGA,G730 BstB ITT`CG,AA3007
Xba IT`CTAG,A736 Bsa IGGTCTC 7/113314
Spe IA`CTAG,T742 HgiE IIACCNNNNNNGGT-1/133654
Bsp120 IG`GGCC,C748 ApaL IG`TGCA,C3929
Apa IG,GGCC`C752  

 

The recommended sequencing primer sequences for pCMV-Blank plasmid are as follows:
T3 primer(620-639): 5′AATTAACCCTCACTAAAGGG 3′
T7 primer(796-817): 5′GTAATACGACTCACTATAGGGC 3′

 

Storage Conditions

Store at -20 °C.


Precautions

  • No commercial use of this plasmid is permitted without written permission from SBS Genetech. Nor shall it be transferred to any individual or institution outside the purchaser’s laboratory.
  • This product is intended solely for scientific‑research use by professionals. It shall not be adopted for clinical diagnosis or therapy, food‑related or pharmaceutical applications, nor kept in residential premises.
  • For your safety and health, wear a lab coat and disposable gloves during operation.

 

 

Only for research and not intended for treatment of humans or animals

 

 

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

 

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