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tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Products 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Enzymes
  • POCT 
    • Integrated POCT Platform
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Lateral Flow System
    • Freeze-Drying System
  • About 
    • About SBS
    • Solutions
    • Achievements
    • Legal Statement
  • Contact
  • …  
    • Home
    • Products 
      • All Products
      • Custom Services
      • Catalog Products
      • Innovative Systems
      • Nucleic Acid Related
      • Enzymes
    • POCT 
      • Integrated POCT Platform
      • LAMP
      • RPA
      • CRISPR
      • DNA-Free Enzymes
      • Lateral Flow System
      • Freeze-Drying System
    • About 
      • About SBS
      • Solutions
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pARE-luc-CP

pARE-luc-CP

pARE-luc-CP (ultra-sensitive rapid degradation reporter plasmid) is a reporter gene plasmid independently developed by our company for rapid response and ultra-sensitive detection of the transcriptional activity level based on the cis-acting element - Antioxidant response element (ARE).
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More Details

All products have special prices for bulk purchase, please contact for more details if required.

 

 

The main information of pARE-luc-CP plasmid is as follows:

  • Base pairs
  • 5808
  • ARE response element (ARE RE)
  • 32-77
  • Luciferase reporter gene
  • 123-1772
  • hCL1
  • 1779-1826
  • hPEST
  • 1830-1949
  • SV40 late poly(A) signal
  • 1005-2126
  • SV40 early enhancer/promoter
  • 2320-2677
  • Synthetic neomycin phosphotransferase (Neor) coding region
  • 2708-3502
  • Synthetic poly(A) signal
  • 3527-3575
  • Reporter Vector primer 4 (RVprimer4) binding region
  • 3642-3661
  • ColE1-derived plasmid replication origin
  • 3899
  • Ampicillin
  • 4690-5550
  • Synthetic poly(A) signal/transcriptional pause site
  • 5655-5808
  • Reporter Vector primer 3 (RVprimer3) binding region
  • 5757-5776

The map of pARE-luc-CP plasmid is as follows:

pARE-luc-CP

 

 

Restriction sites not found in pARE-luc-CP include:

Aat IIAsc IAse IBsa IBsaA IBsiW IBspM II
BssH IIEco72 IEcoR IEcoR VMlu INde INru I
PflM IPme IPml IPsp1406 IPspA IRsr IISac I
Sma ISnaB ISpl ISrf ITth111 IVsp IXcm I
Xma I
  

The single restriction sites in pARE-luc-CP include:

Sfi IGGCCN,NNN`NGGCC8Stu IAGG|CCT2661
Bgl IGCCN,NNN`NGGC8EcoN ICCTNN`N,NNAGG3182
Acc65 IG`GTAC,C14BstB ITT`CG,AA3577
Asp718G`GTAC,C14Sal IG`TCGA,C3591
Kpn IG,GTAC`C18Afl IIIA`CRYG,T3841
Nhe IG`CTAG,C20ApaL IG`TGCA,C4155
PaeR7 IC`TCGA,G26Not IGC`GGCC,GC4661
Xho IC`TCGA,G26BstX ICCAN,NNNN`NTGG4685
Bgl IIA`GATC,T78BstE IIG`GTNAC,C4688
Hind IIIA`AGCT,T88Ahd IGACNN,N`NNGTC4763
BsrG IT`GTAC,A613Bsu36 ICC`TNA,GG5119
Gsu ICTGGAG 21/191503Pvu ICG,AT`CG5133
Bpm ICTGGAG 22/201503Sac IICC,GC`GG5157
Afl IIA`CRYG,T1802Bst1107 IGTA|TAC5273
Mun IC`AATT,G2135Spe IA`CTAG,T5592
BamH IG`GATC,C2228BsmB ICGTCTC 7/115605
 
 

The recommended sequencing primer sequences for pARE-luc-CP plasmid are as follows:

    RVprimer3 (5757-5776): CTA GCA AAA TAG GCT GTC CC

Storage Conditions

Store at -20 °C.


Precautions

  • No commercial use of this plasmid is permitted without written permission from SBS Genetech. Nor shall it be transferred to any individual or institution outside the purchaser’s laboratory.
  • This product is intended solely for scientific‑research use by professionals. It shall not be adopted for clinical diagnosis or therapy, food‑related or pharmaceutical applications, nor kept in residential premises.
  • For your safety and health, wear a lab coat and disposable gloves during operation.

 

 

Only for research and not intended for treatment of humans or animals

 

 

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

 

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