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tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Products 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Enzymes
  • POCT 
    • Integrated POCT Platform
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Lateral Flow System
    • Freeze-Drying System
  • About 
    • About SBS
    • Solutions
    • Achievements
    • Legal Statement
  • Contact
  • …  
    • Home
    • Products 
      • All Products
      • Custom Services
      • Catalog Products
      • Innovative Systems
      • Nucleic Acid Related
      • Enzymes
    • POCT 
      • Integrated POCT Platform
      • LAMP
      • RPA
      • CRISPR
      • DNA-Free Enzymes
      • Lateral Flow System
      • Freeze-Drying System
    • About 
      • About SBS
      • Solutions
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pARE-luc

pARE-luc

pARE-luc (reporter gene plasmid) is a reporter gene plasmid independently developed by our company for detecting the level of transcriptional activity based on the cis-acting element antioxidant response element (ARE, antioxidant response element).
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More Details

All products have special prices for bulk purchase, please contact for more details if required.

 

 

The main information of pARE-luc plasmid is as follows:

  • Base pairs
  • 5622
  • ARE response element(ARE RE)
  • 32-77
  • luc2 reporter gene
  • 123-1775
  • SV40 late poly(A)signal
  • 1810-2031
  • SV40 early enhancer/promoter
  • 2079-2497
  • Synthetic neomycin phosphotransferase
  •  
  • (Neor)coding region
  • 2522-3316
  • Synthetic poly(A)signal
  • 3341-3389
  • Reporter Vector primer 4(RVprimer4)binding region
  • 3456-3475
  • ColE1-derived plasmid replication origin
  • 3713
  • Synthetic Beta-lactamase(Ampr)coding region
  • 4504-5364
  • Synthetic poly(A)signal/transcriptional pause site
  • 5469-5622
  • Reporter Vector primer 3(RVprimer3)binding region
  • 5571-5590

The map of pARE-luc plasmid is as follows:

 

pARE-luc

 

Restriction enzymes that do not cut pARE-luc include:

Aat IIAfl II Asc IAse IBsa IBsaA IBsiW I
BspM IIBssH II Eco72 IEcoR IEcoR VMlu INde I
Nru IPflM I Pme IPml IPsp1406 IPspA IRsr II
Sac ISma I SnaB ISpl ISrf ITth111 IVsp I
Xcm IXma I
  

Restriction enzymes that cut pARE-luc once include:

Sfi IGGCCN,NNN`NGGCC9 EcoN ICCTNN`N,NNAGG2997
Bgl IGCCN,NNN`NGGC9 BsiC ITT`CG,AA3392
Acc65 IG`GTAC,C15 BstB ITT`CG,AA3392
Asp718G`GTAC,C15 Sal IG`TCGA,C3406
Kpn IG,GTAC`C19 Afl IIIA`CRYG,T3656
Nhe IG`CTAG,C21 ApaL IG`TGCA,C3970
PaeR7 IC`TCGA,G27 HgiE IIACCNNNNNNGGT -1/134235
Xho IC`TCGA,G27 Not IGC`GGCC,GC4476
Bgl IIA`GATC,T79 BstX ICCAN,NNNN`NTGG4500
Hind IIIA`AGCT,T89 BstE IIG`GTNAC,C4503
BsrG IT`GTAC,A614 Ahd IGACNN,N`NNGTC4578
Dra IIICAC,NNN`GTG1270 Bsu36 ICC`TNA,GG4934
Gsu ICTGGAG 21/191503 Pvu ICG,AT`CG4948
Bpm ICTGGAG 22/201504 Sac IICC,GC`GG4972
Apo IR`AATT,Y1886 Bst1107 IGTA|TAC5088
Mun IC`AATT,G1950 Xca IGTA|TAC5088
BamH IG`GATC,C2043 Spe IA`CTAG,T5407
Stu IAGG|CCT2476 BsmB ICGTCTC 7/115420
 
 

The recommended sequencing primer sequences for pARE-luc plasmid are as follows:

    RVprimer3(5571-5590):
    CTA GCA AAA TAG GCT GTC CC

Storage Conditions

Store at -20 °C.


Precautions

  • No commercial use of this plasmid is permitted without written permission from SBS Genetech. Nor shall it be transferred to any individual or institution outside the purchaser’s laboratory.
  • This product is intended solely for scientific‑research use by professionals. It shall not be adopted for clinical diagnosis or therapy, food‑related or pharmaceutical applications, nor kept in residential premises.
  • For your safety and health, wear a lab coat and disposable gloves during operation.

 

 

Only for research and not intended for treatment of humans or animals

 

 

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

 

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