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pARE-GLuc-Dura

pARE-GLuc-Dura

pARE-GLuc-Dura (reporter gene plasmid) is a reporter gene plasmid independently developed by our company for detecting the level of transcriptional activity based on the cis-acting element antioxidant response element (ARE, antioxidant response element).
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The main information of pARE-GLuc-Dura plasmid is as follows:

  • Feature Nucleotide
  • Position
  • ARE response element (ARE RE)
  • 32-77
  • GLuc (MT) reporter gene
  • 123-680
  • SV40 late poly (A) signal
  • 715-936
  • SV40 early enhancer/promoter
  • 984-1402
  • Synthetic neomycin phosphotransferase (Neor) coding region
  • 1427-2221
  • Synthetic poly (A) signal
  • 2246-2294
  • Reporter Vector primer 4 (RVprimer4) binding region
  • 2361-2380
  • ColE1-derived plasmid replication origin
  • 2618
  • Synthetic Beta-lactamase (Ampr) coding region
  • 3409-4269
  • Synthetic poly (A) signal/transcriptional pause site
  • 4374-4527
  • Reporter Vector primer 3 (RVprimer3) binding region
  • 4476-4495

The map of pARE-GLuc-Dura plasmid (4527bp) is as follows:

pARE-GLuc-Dura

 

 

Restriction enzymes that do not cut pARE-GLuc-Dura include:

AatIIAclIAflIIAscIAseIAsiSIBmgBI
BsaAIBsaIBsiWIBspEIBsrGIBssHIICspCI
DraIIIEco53kIEcoRIEcoRVMluINdeIPacI
PflFIPflMIPmeIPmlIPspXIRsrIISacI
SbfISmaISnaBISrfISwaITspMITth111I
 
  

The single restriction enzyme cleavage sites (Restriction enzymes that cut pARE-GLuc-Dura) in pARE-GLuc-Dura include:

Acc65IG`GTAC,C14BstZ17IGTA|TAC3992
AflIIIA`CRYG,T2560Bsu36ICC`TNA,GG3838
AgeIA`CCGG,T2231EcoNICCTNN`N,NNAGG1901
AleICACNN|NNGTG3402Esp3ICGTCTCN`NNNN,4324
ApaIG,GGCC`C1497FspITGC|GCA985
ApoIR`AATT,Y790HindIIIA`AGCT,T89
AvaIC`YCGR,G26HpaIGTT|AAC845
BaeI,(N)5`(N)10ACNNNNGTAYC(N)7,(N)5`1627KpnIG,GTAC`C19
BamHIG`GATC,C947MfeIC`AATT,G854
BbvCICC`TCA,GC2112NheIG`CTAG,C21
BciVIGTATCC(N)5,N`2762NotIGC`GGCC,GC3380
BglIGCCN,NNN`NGGC9NruITCG|CGA228
BglIIA`GATC,T79PaeR7IC`TCGA,G26
BmtIG,CTAG`C20PciIA`CATG,T2560
BpmICTGGAG(N)14,NN`301PsiITTA|TAA825
BsaXI,NNN`(N)9AC(N)5CTCC(N)7,NNN`115PspOMIG`GGCC,C1497
BsmBICGTCTCN`NNN,N4324PvuIICAG|CTG1057
BsoBIC`YCGR,G26SfiIGGCCN,NNN`NGGCC5
BspHIT`CATG,A3280SgrAICR`CCGG,YG1643
BssSIC`ACGA,G2733SpeIA`CTAG,T4311
BstBITT`CG,AA2296StuIAGG|CCT1380
BstEIIG`GTNAC,C3407XhoIC`TCGA,G27
BstXICCAN,NNNN`NTGG3400XmnIGAANN|NNTTC384


The recommended sequencing primer sequences for pARE-luc plasmid are as follows:

RVprimer3 (4476-4495): CTA GCA AAA TAG GCT GTC CC


Storage Conditions

Store at -20 °C.


Precautions

  • No commercial use of this plasmid is permitted without written permission from SBS Genetech. Nor shall it be transferred to any individual or institution outside the purchaser’s laboratory.
  • This product is intended solely for scientific‑research use by professionals. It shall not be adopted for clinical diagnosis or therapy, food‑related or pharmaceutical applications, nor kept in residential premises.
  • For your safety and health, wear a lab coat and disposable gloves during operation.

 

 

Only for research and not intended for treatment of humans or animals

 

 

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

 

 

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

 

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