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tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Products 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Enzymes
  • POCT 
    • Integrated POCT Platform
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Lateral Flow System
    • Freeze-Drying System
  • About 
    • About SBS
    • Solutions
    • Achievements
    • Legal Statement
  • Contact
  • …  
    • Home
    • Products 
      • All Products
      • Custom Services
      • Catalog Products
      • Innovative Systems
      • Nucleic Acid Related
      • Enzymes
    • POCT 
      • Integrated POCT Platform
      • LAMP
      • RPA
      • CRISPR
      • DNA-Free Enzymes
      • Lateral Flow System
      • Freeze-Drying System
    • About 
      • About SBS
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pAP1-TA-luc-CP

pAP1-TA-luc-CP

pAP1-TA-luc-CP (ultra-sensitive rapid degradation reporter plasmid) is a reporter gene plasmid independently developed by our company for rapid response and ultra-sensitive detection of AP1 transcriptional activity levels.
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More Details

All products have special prices for bulk purchase, please contact for more details if required.

 

 

The main information of pAP1-TA-luc-CP plasmid is as follows:

  • Base pairs
  • 5839
  • AP1 response element
  • 26-67
  • Minimal TA promoter (pTA)
  • 90-121
  • Luciferase reporter gene
  • 154-1803
  • hCL1
  • 1810-1857
  • hPEST
  • 1862-1981
  • SV40 late poly(A) signal
  • 2036-2157
  • SV40 early enhancer/promoter
  • 2351-2708
  • Neomycin
  • 2739-3533
  • Synthetic poly(A) signal
  • 3558-3606
  • Reporter Vector primer 4 (RVprimer4) binding region
  • 3673-3692
  • ColE1-derived plasmid replication origin
  • 3930
  • Ampicillin
  • 4721-5581
  • Synthetic poly(A) signal/transcriptional pause site
  • 5686-5839
  • Reporter Vector primer 3 (RVprimer3) binding region
  • 5788-5807


The map of pAP1-TA-luc-CP plasmid is as follows:

pAP1-TA-luc-CP

 

 

Restriction sites not found in pAP1-TA-luc-CP include:

Aat IIAsc IAse IBsa IBsaA IBsiW IBspM II
BssH IIEco72 IEcoR IEcoR VMlu INde INru I
PaeR7 IPflM IPme IPml IPsp1406 IPspA IRsr II
Sac ISma ISnaB ISpl ISrf ITth111 IVsp I
Xcm IXho IXma I 
  

The single restriction sites in pAP1-TA-luc-CP include:

Sfi IGGCCN,NNN`NGGCC8BstB ITT`CG,AA3608
Bgl IGCCN,NNN`NGGC8Sal IG`TCGA,C3622
Acc65 IG`GTAC,C14Afl IIIA`CRYG,T3872
Asp718G`GTAC,C14ApaL IG`TGCA,C4186
Kpn IG,GTAC`C18Not IGC`GGCC,GC4692
Nhe IG`CTAG,C20BstX ICCAN,NNNN`NTGG4716
Bgl IIA`GATC,T68BstE IIG`GTNAC,C4719
Hind IIIA`AGCT,T78Ahd IGACNN,N`NNGTC4794
BsrG IT`GTAC,A644Bsu36 ICC`TNA,GG5150
Gsu ICTGGAG 21/191534Pvu ICG,AT`CG5164
Bpm ICTGGAG 22/201534Sac IICC,GC`GG5188
Afl IIA`CRYG,T1833Bst1107 IGTA|TAC5304
Mun IC`AATT,G2166Spe IA`CTAG,T5623
BamH IG`GATC,C2259BsmA IGTCTC`/95636
Stu IAGG|CCT2692BsmB ICGTCTC 7/115636
 
 

The recommended sequencing primer sequences for pAP1-TA-luc-CP plasmid are as follows:

    RVprimer3 (5788-5807): CTA GCA AAA TAG GCT GTC CC

Storage Conditions

Store at -20 °C.


Precautions

  • No commercial use of this plasmid is permitted without written permission from SBS Genetech. Nor shall it be transferred to any individual or institution outside the purchaser’s laboratory.
  • This product is intended solely for scientific‑research use by professionals. It shall not be adopted for clinical diagnosis or therapy, food‑related or pharmaceutical applications, nor kept in residential premises.
  • For your safety and health, wear a lab coat and disposable gloves during operation.

 

 

Only for research and not intended for treatment of humans or animals

 

 

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

 

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