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tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Products 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Enzymes
  • POCT 
    • Integrated POCT Platform
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Lateral Flow System
    • Freeze-Drying System
  • About 
    • About SBS
    • Solutions
    • Achievements
    • Legal Statement
  • Contact
  • …  
    • Home
    • Products 
      • All Products
      • Custom Services
      • Catalog Products
      • Innovative Systems
      • Nucleic Acid Related
      • Enzymes
    • POCT 
      • Integrated POCT Platform
      • LAMP
      • RPA
      • CRISPR
      • DNA-Free Enzymes
      • Lateral Flow System
      • Freeze-Drying System
    • About 
      • About SBS
      • Solutions
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pAP1-TA-luc

pAP1-TA-luc

pAP1-TA-luc (reporter gene plasmid) is a reporter gene plasmid independently developed by our company for detecting AP1 transcription activity levels. pAP1-TA-luc uses Beyotime's pGL6-TA plasmid as a template and inserts multiple AP1 binding sites into its multiple cloning site, which can detect the activation level of AP1 with high sensitivity.
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More Details

All products have special prices for bulk purchase, please contact for more details if required.

 

 

The main information of pAP1-TA-luc plasmid is as follows:

  • Base pairs
  • 5653
  • AP1 response element
  • 26-67
  • Minimal TA promoter (pTA)
  • 90-112
  • luc2 reporter gene
  • 154-1806
  • SV40 late poly(A) signal
  • 1841-2062
  • SV40 early enhancer/promoter
  • 2110-2528
  • Synthetic neomycin phosphotransferase
  •  
  • (Neor) coding region
  • 2553-3347
  • Synthetic poly(A) signal
  • 3372-3420
  • Reporter Vector primer 4 (RVprimer4) binding region
  • 3487-3506
  • ColE1-derived plasmid replication origin
  • 3744
  • Synthetic Beta-lactamase (Ampr) coding region
  • 4535-5395
  • Synthetic poly(A) signal/transcriptional pause site
  • 5500-5653
  • Reporter Vector primer 3 (RVprimer3) binding region
  • 5602-5621

The map of pAP1-TA-luc plasmid is as follows:

pAP1-TA-luc

 

Restriction enzymes that do not cut pAP1-TA-luc include:

Aat IIAfl IIAsc IAse IBsa IBsaA IBsiW IBspM II
BssH IIEco72 IEcoR IEcoR VMlu INde INru IPaeR7 I
PflM IPme IPml IPsp1406 IPspA IRsr IISac ISma I
SnaB ISpl ISrf ITth111 IVsp IXcm IXho IXma I
  

Restriction enzymes that cut pAP1-TA-luc once include:

Sfi IGGCCN,NNN`NGGCC9 BstB ITT`CG,AA3423
BglIGCCN,NNN`NGGC9 Sal IG`TCGA,C3437
Acc65 IG`GTAC,C15 Afl IIIA`CRYG,T3687
Asp718G`GTAC,C15 ApaL IG`TGCA,C4001
Kpn IG,GTAC`C19 HgiE IIACCNNNNNNGGT -1/134266
Nhe IG`CTAG,C21 Not IGC`GGCC,GC4507
Bgl IIA`GATC,T69 BstX ICCAN,NNNN`NTGG4531
Hind IIIA`AGCT,T79 BstE IIG`GTNAC,C4534
BsrG IT`GTAC,A645 Ahd IGACNN,N`NNGTC4609
Dra IIICAC,NNN`GTG1301 Bsu36 ICC`TNA,GG4965
Gsu ICTGGAG 21/191534 Pvu ICG,AT`CG4979
Bpm ICTGGAG 22/201535 Sac IICC,GC`GG5003
Apo IR`AATT,Y1917 Bst1107 IGTA|TAC5119
Mun IC`AATT,G1981 Xca IGTA|TAC5119
BamH IG`GATC,C2074 Spe IA`CTAG,T5438
Stu IAGG|CCT2507 BsmA IGTCTC`/95450
EcoN ICCTNN`N,NNAGG3028 BsmB ICGTCTC 7/115451
BsiC ITT`CG,AA3423
 
 

The recommended sequencing primer sequences for pAP1-TA-luc plasmid are as follows:

    RVprimer3 (5602-5621):
    CTA GCA AAA TAG GCT GTC CC

Storage Conditions

Store at -20 °C.


Precautions

  • No commercial use of this plasmid is permitted without written permission from SBS Genetech. Nor shall it be transferred to any individual or institution outside the purchaser’s laboratory.
  • This product is intended solely for scientific‑research use by professionals. It shall not be adopted for clinical diagnosis or therapy, food‑related or pharmaceutical applications, nor kept in residential premises.
  • For your safety and health, wear a lab coat and disposable gloves during operation.

 

 

Only for research and not intended for treatment of humans or animals

 

 

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

 

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