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tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Products 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Enzymes
  • POCT 
    • Integrated POCT Platform
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Lateral Flow System
    • Freeze-Drying System
  • About 
    • About SBS
    • Solutions
    • Achievements
    • Legal Statement
  • Contact
  • …  
    • Home
    • Products 
      • All Products
      • Custom Services
      • Catalog Products
      • Innovative Systems
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      • Integrated POCT Platform
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p35SPPDK-EGFP-Flag

p35SPPDK-EGFP-Flag

p35SPPDK-EGFP-Flag (green fluorescent protein for plants) is a self-developed plant (such as Arabidopsis, etc.) protoplast expression plasmid. It can be used as a positive control plasmid for transient transfection of protoplasts or to observe the subcellular localization of the target protein by constructing an EGFP fusion protein.
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All products have special prices for bulk purchase, please contact for more details if required.

 

 

The main information of p35SPPDK-EGFP-Flag plasmid is as follows:

  • Feature Nucleotide
  • Position
  • M13 forward primer(M13 fwd)
  • 1-17
  • 35SPPDK promoter
  • 84-593
  • EGFP
  • 617-1333
  • 2X Flag-tag
  • 1355-1402
  • NOS terminator
  • 1411-1663
  • M13 reverse primer(M13 rev)
  • 1702-1718
  • lac operator
  • 1726-1742
  • lac promoter
  • 1750-1780
  • CAP binding site
  • 1795-1816
  • pUC origin
  • 2104-2692
  • Ampicililin resistance ORF
  • 2863-3723
  • Ampicililin promoter
  • 3724-3828

The map of p35SPPDK-EGFP-Flag plasmid (4301bp) is as follows:

p35SPPDK-EGFP-Flag

 

Restriction enzymes that do not cut p35SPPDK-EGFP-Flag include:

AccIAfeIAgeIAscIAsiSIAvrIIBbvCI
BclIBfuAIBglIIBlpIBmtIBsiWIBspDI
BspMIBstBIBstEIIBstXIBstZ17IBsu36IClaI
DraIIIEagIEcoNIFseIHincIIHpaIMfeI
MluIMscINaeINgoMIVNheINotINruI
PacIPflFIPflMIPmeIPmlIPpuMIPsiI
PspXIRsrIISacIISalISbfISexAISfiI
SgrAISmaISnaBISphISrfISwaITspMI
Tth111IXcmIXmaI


The single restriction enzyme cutting sites (Restriction enzymes that cut p35SPPDK-EGFP-Flag once) in p35SPPDK-EGFP-Flag include:

AatIIG,ACGT`C3858EcoRVGAT|ATC337
Acc65IG`GTAC,C611KasIG`GCGC,C4158
AflIIA`CRYG,T1440KpnIG,GTAC`C616
AhdIGACNN,N`NNGTC2936NarIGG`CG,CC4159
AlwNICAG,NNN`CTG2459NcoIC`CATG,G616
ApaIG,GGCC`C396NdeICA`TA,TG4108
ApoIR`AATT,Y1687NsiIA,TGCA`T1531
AvaIC`YCGR,G26PaeR7IC`TCGA,G26
BaeI,(N)5`(N)1ACNNNNGTAYC(N)7,(N)5`598PluTIG,GCGC`C4162
BamHIG`GATC,C597PshAIGACNN|NNGTC245
BmgBICAC|GTC308PspOMIG`GGCC,C392
BsaAIYAC|GTR556PstIC,TGCA`G1411
BsaBIGATNN|NNATC1474SacIG,AGCT`C523
BsaIGGTCTCN`NNNN,2997SapIGCTCTTCN`NNN,1927
BsmIG`AATG,CN`437ScaIAGT|ACT3417
BsoBIC`YCGR,G26SfoIGGC|GCC4160
BspEIT`CCGG,A527SpeIA`CTAG,T1335
BspQIGCTCTTCN`NNN,1927SspIAAT|ATT3740
BsrGIT`GTAC,A1326StuIAGG|CCT1349
BstAPIGCAN,NNN`NTGC4105StyIC`CWWG,G615
CspCINN`(N)11CAA(N)5GTGG(N)10,NN`300XbaIT`CTAG,A603
Eco53kIGAG|CTC525XhoIC`TCGA,G27
EcoO109IRG`GNC,CY3912ZraIGAC|GTC3856
EcoRIG`AATT,C1688


The sequencing primer sequences that can be used for p35SPPDK-EGFP-Flag plasmid are as follows:

35SPPDK forward primer(473-494): 5'-CCTTTTCGCTGTACTCTCTCGC-3'

EGFP reverse primer(670-689): 5'-CGTTTACGTCGCCGTCCAG-3'


Storage Conditions

Store at -20 °C.


Precautions

  • No commercial use of this plasmid is permitted without written permission from SBS Genetech. Nor shall it be transferred to any individual or institution outside the purchaser’s laboratory.
  • This product is intended solely for scientific‑research use by professionals. It shall not be adopted for clinical diagnosis or therapy, food‑related or pharmaceutical applications, nor kept in residential premises.
  • For your safety and health, wear a lab coat and disposable gloves during operation.

 

 

Only for research and not intended for treatment of humans or animals

 

 

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