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tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.
broken image

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Product 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Natural Compounds
    • Synthetic Biology
    • Enzymes
  • POCT Solution 
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Freeze-Drying System
    • Lateral Flow System
  • About 
    • About SBS
    • Achievements
    • Ecosystem
    • Legal Statement
  • Contact
  • …  
    • Home
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      • All Products
      • Custom Services
      • Catalog Products
      • Innovative Systems
      • Nucleic Acid Related
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      • LAMP
      • RPA
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      • Freeze-Drying System
      • Lateral Flow System
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Mouse CRISPR Knockout Pooled Library (Single Plasmid System A+B)

Mouse CRISPR Knockout Pooled Library (Single Plasmid System A+B)

Mouse CRISPR Knockout Pooled Library (Single Plasmid System A+B) is designed for whole-genome knockout and screening in mice, targeting a total of 20,611 genes. The library comprises 130,209 knockout vectors, with each gene targeted by six gRNA knockout vectors. Additionally, there are 1,000 control vectors (including 1,000 targeting non-genic sequences). This library utilizes the lentiCRISPR v2 backbone, an all-in-one vector system where gRNA and Cas9 genes are on the same vector, allowing for standalone use.
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Description

Mouse CRISPR Knockout Pooled Library (Single Plasmid System A+B) is designed for whole-genome knockout and screening in mice, targeting a total of 20,611 genes. The library comprises 130,209 knockout vectors, with each gene targeted by six gRNA knockout vectors. Additionally, there are 1,000 control vectors (including 1,000 targeting non-genic sequences). This library utilizes the lentiCRISPR v2 backbone, an all-in-one vector system where gRNA and Cas9 genes are on the same vector, allowing for standalone use.

  • Species: Mouse

  • Library Type: Knockout Library

  • Plasmid System: Single Plasmid System

  • Virus Packaging System: Third Generation Virus Packaging System

  • Target Genes: 20,611

  • Number of gRNAs: 130,209

  • Non-target Control sgRNAs: 1,000

  • Tag: Puro

 

 

Featured Citations

Interested in seeing published research using our gRNAs?

DDR1 promotes breast tumor growth by suppressing antitumor immunity

Oncology Reports    |    27 September 2019    |    DOI: https://doi.org/10.3892/or.2019.7338

Oligonucleotides with BsmB1 restriction sites for guide RNAs were synthesized by SBS Genetech Co., Ltd., then phosphorylated using T4 kinase (31). The phosphorylated oligonucleotides were cloned into LentiCRISPR v2 (Plasmid no. 52961; Addgene) and the sequences of the cloned plasmids that were extracted from numerous selected colonies were confirmed by SBS Genetech Co., Ltd.

 

 

Only for research and not intended for treatment of humans or animals

 

 

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

 

 

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

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