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Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.
broken image

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Product 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Natural Compounds
    • Synthetic Biology
    • Enzymes
  • POCT Solution 
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Freeze-Drying System
    • Lateral Flow System
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    • About SBS
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    • Ecosystem
    • Legal Statement
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Human CRISPR Whole Genome Knockout Library (Dual Vector System A+B)

Human CRISPR Whole Genome Knockout Library (Dual Vector System A+B)

This knockout library is suitable for whole-genome knockout and screening in humans, targeting 19,050 human genes and comprising a total of 123,411 knockout vectors. Each gene is targeted by six gRNA knockout vectors, with an additional 1,000 control vectors targeting non-genomic sequences. The library uses the LentiGuide-Puro backbone and operates as a dual-vector system, expressing only the gRNA. The Cas9 gene is on a separate vector and needs to be used in conjunction.
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Description

This knockout library is suitable for whole-genome knockout and screening in humans, targeting 19,050 human genes and comprising a total of 123,411 knockout vectors. Each gene is targeted by six gRNA knockout vectors, with an additional 1,000 control vectors targeting non-genomic sequences. The library uses the LentiGuide-Puro backbone and operates as a dual-vector system, expressing only the gRNA. The Cas9 gene is on a separate vector and needs to be used in conjunction.

Species: Human 

Library Type: Knockout Library 

Plasmid System: Dual Vector System 

Viral Packaging System: Third Generation Viral Packaging System 

Target Genes: 19,050 

Number of gRNAs: 123,411 

Number of Non-Target Control 

sgRNAs: 1,000 

Tag: Puro

 

 

Featured Citations

Interested in seeing published research using our gRNAs?

DDR1 promotes breast tumor growth by suppressing antitumor immunity

Oncology Reports    |    27 September 2019    |    DOI: https://doi.org/10.3892/or.2019.7338

Oligonucleotides with BsmB1 restriction sites for guide RNAs were synthesized by SBS Genetech Co., Ltd., then phosphorylated using T4 kinase (31). The phosphorylated oligonucleotides were cloned into LentiCRISPR v2 (Plasmid no. 52961; Addgene) and the sequences of the cloned plasmids that were extracted from numerous selected colonies were confirmed by SBS Genetech Co., Ltd.

 

 

Only for research and not intended for treatment of humans or animals

 

 

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

 

 

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

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