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High-Throughput Expression Vector Testing
Traditional optimization of heterologous protein expression requires mind-numbingly repetitive work routines with different expression vectors and conditions. Such repeated trial and error not only waste time and money but also often fail to solve problems. At SBS Genetech, with a high throughput expression platform, we can get the suitable expression vector for your protein at low cost and with great efficiency.
More Details
Features
- Billions of combinations of promoters, ribosome binding sites, and terminators are available
- Professional codon optimization and DNA synthesis
- Only 1/50 of the market price of traditional expression vector test
- The leading time is around 5-9 weeks
Comparison
Compared with the traditional expression vector tests, our high-throughput optimization has a much lower cost per test.
Order
Sequence
- Customers can send us either DNA sequence or protein sequence for the testing. For protein sequence, we will provide codon optimization and get the ideal DNA sequence for expression.
Parameter
- Throughput: The number of expression vectors (e.g. 10,000) needs to be randomly tested
- Expression Strains: We support DH5a, DH10B, and BL21 (DE3)
- Experimental Conditions: Overnight culture at either 37°C or 30°C in 250 ml LB
Result
- Top Sequence: The sequence of the vector with the highest protein expression level in the experiment
- Data Plot (Optional): The plot of the average protein expression level and standard deviation in different vectors
- Dataset (Optional): The raw data of protein expression level in different vectors
- Customized Model (Optional): Prediction of the protein expression level in different vectors by artificial intelligence model base on the above dataset
Download the order form here.
Only for research and not intended for treatment of humans or animals
