De novo Third-Generation Sequencing of Plant and Animal Genomes
Description
De novo sequencing analysis of plant and animal genomes, also known as from-scratch sequencing analysis, refers to sequencing analysis of a specific plant or animal without relying on any reference sequence information. This involves using bioinformatics methods to assemble sequences to obtain the genome sequence map of a species, followed by a series of subsequent analyses such as genome structure annotation, functional annotation, and comparative genomics analysis. Third-generation sequencing technologies (represented by PacBio and Nanopore) are characterized by long read lengths and have been making their mark in de novo sequencing of plant and animal genomes since 2015, continuing to be used to this day. The results of this type of sequencing analysis can be widely applied to research in agriculture, forestry, fisheries, animal husbandry, medicine, and marine sciences.
Principles of Third-Generation Sequencing Technology
PacBio Sequencing Principles
PacBio sequencing employs a "sequencing-by-synthesis" approach. It uses one DNA strand as a template, with DNA polymerase synthesizing the complementary strand and converting fluorescent signals into base calls. Additionally, PacBio has upgraded to the CCS (Circular Consensus Sequencing) mode to obtain long, high-fidelity (HiFi) 15 kb reads, thereby enhancing the accuracy of genome assembly.
Nanopore Sequencing Principles
As single-stranded DNA molecules pass through a nanopore, different current signals are generated for each nucleotide. The ion current fluctuations at each pore are recorded and converted into base sequences using methods based on Markov models or recurrent neural networks. Additionally, Ultra-long reads (ULRs) are another significant feature of the ONT platform, offering the potential to facilitate large genome assembly.
Technical Service Process
- Sample Shipping
- Library Construction and Sequencing
- Data Analysis
- Report Issuance
- Post-Sales Support
