Description

We supply peptide library as either desalt or >70%, >80%, >90%, and >95% purities. Peptides can be made in required amounts from minimal to mg scale for initial screening. Appropriate peptide labels (Biotin, FITC, etc.) can be added upon request. Each peptide in the library undergoes rigorous quality control to avoid any cross contaminants before delivery. All purified peptides are delivered with complete QC data including RP-HPLC, MS, and COA reports to ensure high quality. The desalted peptides are provided with MS only. Peptide length in a given library is usually 5-15 aa with 1-5 amino acid overlap.

Peptide Library Types

Depending upon a given application, peptide libraries are usually defined as:

A. Overlapping Peptide Library

Overlapping peptide library can be used for epitope mapping. The peptide length (5-15aa) and offset number or the degree of overlapping amino acids (2-5 aa) is determined by the length of the full-length proteins, overall experiment objectives, and the cost. As a general guideline, shorter peptides have less chance for multiple epitope hits. A greater degree of overlapping (small offset number) gives more chances of multiple epitope hits.

Example:   (peptide length 10 aa, offset/overlap 3 aa)

Total peptides, n=11

B. Alanine Scanning Library

Example:   (peptide length 10 aa)

C. Truncation Library

A truncated peptide library can be used to identify the shortest amino acid sequence needed for a given function or activity.  

Example:   (peptide length 10 aa) Truncation from the N-terminus

Truncated peptides can also be generated from the C-Terminus.

D. Random/scrambled Library

Scramble Library is constructed by carrying out permutation on the original peptide’s sequence. The number of each amino acid (e.g. 2 Arg or R) is the same in the peptide except its position and other amino acids are created at random. This strategy is used for preliminary identification of a group of active sequences that may possess a given function or activity.

E. Positional Scanning Library

A selected position or positions in a peptide sequence are each systematically replaced with all other natural amino acids (20) or any other amino acid to determine the preferred amino acid residues at a given position that will maximize the peptide activity.

Example:   peptide length 10 aa, with substitution of “K at position 5.

Potential peptides =20 with all 20 natural amino acids. It is also possible to study the replacement of “N” at position #8.

Peptide Library Pricing

Peptide libraries are synthesized on SBS Genetech’s unique proprietary synthesis platform and are typically supplied at the 2-4 mg scale for fast, efficient screening work.  

Peptide library pricing (per peptide) for up to 15 amino acids.

• Desalted peptides are provided with MS only.
• Purified peptides are provided with MS and HPLC data.

Related:

We also provide Custom Micropeptide Library Services. Unlike conventional peptide libraries that provide milligram-level peptides, micropeptide libraries contain microgram-level peptides. They are cost-effective and more suitable for initial screening in applications such as targeted proteomics, target screening, and antigen epitope identification.

With more than 20 years of experience in solid-phase peptide synthesis, SBS Genetech is recognized as one of the global major leading industry players in Peptide Synthesis by third-party market researchers. For more details, please visit Peptide Synthesis Market 2017-2022 International Outlook, Trends and Analysis.

Featured Publications

• Yang M, Chen J, Li X, Huang J, Wang Q, Wang S, Wei S, Qin Q. The transcription factor NFYC positively regulates expression of MHCIa in the red-spotted grouper (Epinephelus akaara). Dev Comp Immunol. 2021 Sep 29;127:104272. 
• Wu X, Li Y, Xu H, Chen Y, Mao H, Ma Q, Du Q, Gao P, Xia F. Exponential Increase in an Ionic Signal: A Dominant Role of the Space Charge Effect on the Outer Surface of Nanochannels. Anal Chem. 2021 Sep 28.
• Yaghoubi P, Bandehpour M, Mohebali M, Akhoundi B, Kazemi B. Designing and Evaluation of a Recombinant Multiepitope Protein by Using ELISA for Diagnosis of Leishmania infantum Infected in Dogs. Iran J Parasitol. 2021 Jul-Sep;16(3):377-385. 
• Xiao PP, Wan QQ, Liao T, Tu JY, Zhang GJ, Sun ZY. Peptide Nucleic Acid-Functionalized Nanochannel Biosensor for the Highly Sensitive Detection of Tumor Exosomal MicroRNA. Anal Chem. 2021 Aug 10;93(31):10966-10973. 
• Georgieva M, Heinonen T, Vitale A, Hargraves S, Causevic S, Pillonel T, Eberl L, Widmann C, Jacquier N. Bacterial surface properties influence the activity of the TAT-RasGAP_317-326 antimicrobial peptide. iScience. 2021 Jul 30;24(8):102923.
• Xu R, Li Y, Sui Z, Lan T, Song W, Zhang M, Zhang Y, Xing J. A C-terminal encoded peptide, ZmCEP1, is essential for kernel development in maize. J Exp Bot. 2021 Jul 28;72(15):5390-5406. doi: 10.1093/jxb/erab224. PMID: 34104938.
• Gu H, Ma K, Zhao W, Qiu L, Xu W. A general purpose MALDI matrix for the analyses of small organic, peptide and protein molecules. Analyst. 2021 Jun 14;146(12):4080-4086.
• Dong L, Tan CW, Feng PJ, Liu FB, Liu DX, Zhou JJ, Chen Y, Yang XX, Zhu YH, Zhu ZQ. Activation of TREM-1 induces endoplasmic reticulum stress through IRE-1α/XBP-1s pathway in murine macrophages. Mol Immunol. 2021 Jul;135:294-303.
• Heinonen T, Hargraves S, Georgieva M, Widmann C, Jacquier N. The antimicrobial peptide TAT-RasGAP_317-326 inhibits the formation and expansion of bacterial biofilms in vitro. J Glob Antimicrob Resist. 2021 Jun;25:227-231. 
• Wang Y, Xie X, Liu H, Liu H, Jiang H. LR12 Promotes Liver Repair by Improving the Resolution of Inflammation and Liver Regeneration in Mice with Thioacetamide- (TAA-) Induced Acute Liver Failure. Mediators Inflamm. 2021 May 28;2021:2327721.
• Gong W, Liang Y, Mi J, Jia Z, Xue Y, Wang J, Wang L, Zhou Y, Sun S, Wu X. Peptides-Based Vaccine MP3RT Induced Protective Immunity Against Mycobacterium Tuberculosis Infection in a Humanized Mouse Model. Front Immunol. 2021 Apr 26;12:666290.