Cell-Free Protein Expression System
Description
Cell-Free Protein Expression System, also known as the in vitro translation system, simulates the intracellular protein transcription and translation process by adding amino acids, energy sources, and exogenous DNA or mRNA templates to the reaction mixture. This system is characterized by short cycles and high throughput. In addition to proteins expressed by conventional protein expression systems, the cell-free protein expression system can also produce toxic proteins and other proteins that are difficult to express with conventional systems.
Based on our self-developed enhanced cell-free protein expression system and reaction amplification technology, we offer synthesis services for various recombinant proteins and difficult-to-express proteins. With the extensive experience of our professional technical team in DNA synthesis and molecular cloning, combined with our multifunctional and efficient cell-free protein expression platform, we can deliver products from protein sequence DNA to protein expression and purification in as little as 3 days.
Prokaryotic System
One of the most commonly used and widely applied expression systems, it is cost-effective and can express some recombinant proteins with no glycosylation or minimal modifications. Simply provide us with a protein sequence, and our experienced scientists can develop a complete protein expression and purification plan for you in a short time.
Service Advantages
- Integrated with cell-free protein expression technology platform to ensure timely delivery of proteins.
- Successfully completed hundreds of recombinant protein synthesis projects.
- One-stop service from gene sequence to protein purification.
Service Process
- Sequence Optimization and Synthesis: 5-10 working days
- Molecular Construction: 3-5 working days
- Small-Scale Expression and Optimization: 1-2 working days
- Protein Scale-Up Expression: 3-5 working days
- Protein Purification: 5-10 working days
Deliverables
- Target plasmid (2 µg)
- Protein expression and purification experiment report (experimental protocol and results)
- Target protein product (purity ~90%, endotoxin 1 EU/µg)