Description

AF 555 Maleimide is a bright orange dye that is water-soluble and pH insensitive (pH 4 to pH 10). Its excitation and emission spectra cover a large portion of the visible light and part of the infrared spectrum, making it widely applicable in various biological imaging techniques, such as fluorescence microscopy, flow cytometry, and fluorescence resonance energy transfer. Additionally, it can be used in in vivo imaging experiments to observe physiological processes within organisms.

This fluorescent dye exhibits good photostability and cell permeability, allowing for long-term fluorescence labeling in live cells. Furthermore, it shows very low background fluorescence when combined with antibodies, ensuring the accuracy of experimental results. It is commonly used to label biological molecules such as proteins, antibodies, and nucleic acids.

Applications

AF 555 Maleimide has broad applications in biomedical research. It can be used for the labeling and localization of tissues, cells, and biomolecules. For example, it can connect with click chemistry linkers like DBCO, Azide, Alkyne, and Tetrazine to synthesize a series of AF 555 fluorescent probes for various applications in biomedical research. Moreover, the maleimide derivative of AF 555 is a commonly used tool for coupling this dye to thiol groups on proteins, oligonucleotide phosphorothioates, or low molecular weight ligands. The resulting AF 555 conjugates exhibit brighter fluorescence and greater photostability compared to conjugates of other spectrally similar fluorescent groups.