• Next-Generation HotStart Bst Reagent

    Ultra-Sensitive, Lyophilized Solutions for Reliable DNA/RNA Detection Across Research and Manufacturing

  • It is time for HotStart Bst

    The HotStart Bst reagent series, carefully developed by SBS Genetech, is a high-performance solution for LAMP amplification, compatible with both DNA and RNA target detection. Built on rapid and highly tolerant enzymatic performance, it provides robust, fast, and ultra-sensitive amplification. The integration of HotStart technology effectively reduces false positives by preventing premature activity at lower temperatures.
    Its proprietary lyophilized formulation grants this reagent exceptional versatility—fulfilling standard research needs while enabling seamless development and manufacturing of lyophilized products using a single formulation.
    1

    Thermostable Aptamer for HotStart Control

    The entire LyoMix and lyophilized microbead series incorporates a HotStart aptamer that ensures >90% inhibition of enzyme activity below 30 °C and complete activation within 1 minute above 60 °C. This enables convenient room-temperature setup and substantially reduces nonspecific amplification under low-temperature conditions.

    2

    Elevated Reaction Temperature Enhances Specificity

    The Bst P DNA/RNA Polymerase series supports amplification at 70 °C, which significantly reduces primer-dimer formation, improves amplification specificity, and facilitates more efficient nucleic acid release from crude samples.

    3

    Helicase-Enabled pLAMP Simplifies Primer Design

    Integrated helicase allows for pLAMP (prime LAMP) amplification without the need for F3/B3 outer primers. It also permits halving the amount of FIP/BIP primers used, further reducing nonspecific amplification and greatly improving amplification uniformity.

    4

    Ready-to-Lyophilize Formulation for Versatile Application

    Supplied as a pre-lyomix liquid formulation, the reagent offers exceptional versatility—supporting routine research use and enabling streamlined development and large-scale production of lyophilized products within a single formulation system.

  • HotStart Bst DNA/RNA v.s. HotStart Bst P DNA/RNA

    Both Bst DNA/RNA Polymerase and Bst P DNA/RNA Polymerase demonstrate rapid amplification capabilities; however, in most cases, Bst P DNA/RNA Polymerase delivers noticeably faster amplification under identical primer conditions. Thanks to its elevated reaction temperature (70 °C), it also offers higher specificity, improved sensitivity, and reduced primer consumption. Therefore, for new projects, it is recommended to begin with the Bst P DNA/RNA Polymerase system for testing and optimization. If the desired detection performance is not achieved, the Bst4.0 system can be used as an alternative.

    Interestingly, some primer sets perform particularly well at 65 °C for reasons that are not yet fully understood—this preference does not appear to correlate with primer Tm or GC content. Currently, as LAMP amplification at 70 °C is a proprietary technology unique to SBS Genetech, most commercial LAMP reagents from other suppliers operate within the 60–65 °C range. As such, primer sets screened under these conditions can be seamlessly adapted to the Bst DNA/RNA LyoMix system.

    Feature

    Hotstart Mechanism

    Reaction Temperature

    Number of Primers

    F3/B3 Primers Required

    Target Substrates

    Amplification Specificity

    Amplification Speed

    HotStart Bst P DNA/RNA LyoMix

    Aptamer-based inhibition

    70 °C

    4-6

    Not required

    DNA/RNA

    Optimal

    ⭐⭐⭐⭐⭐⭐

    HotStart Bst DNA/RNA LyoMix

    Aptamer-based inhibition

    65 °C

    5-6

    Required

    DNA/RNA

    Good

    ⭐⭐⭐⭐⭐

  •  

    Ready for this new experience?

     

  • HotStart Bst Reagents

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    PrimeIAmp™ HotStart Bst dU-P Fluorescent Microbeads (with UDG)
    $500.00 - $2,000.00
    $2,500.00
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    PrimeIAmp™ HotStart Bst dU-P Basic Microbeads (with UDG)
    $500.00 - $2,000.00
    $2,500.00
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    Bst dU‑P Fluorescent LAMP Lyophilized Mix
    $400.00 - $1,600.00
    $2,000.00
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    Bst dU‑P Basic LAMP Lyophilized Mix
    $400.00 - $1,600.00
    $2,000.00
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    LAMP Loop DP-Probe Pair
    $600.00
    $750.00
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    PrimeIAmp™ HotStart Bst P DNA/RNA LHNB LyoMix (70ºC reaction)
    $660.00 - $2,000.00
    $2,500.00
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    PrimeIAmp™ HotStart Bst P DNA/RNA Basic & SYBR Green LyoMix (70ºC reaction)
    $660.00 - $2,000.00
    $2,500.00
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    PrimeIAmp™ HotStart Bst P DNA/RNA LHNB Microbead (70ºC reaction)
    $740.00
    $925.00
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    PrimeIAmp™ HotStart Bst P DNA/RNA Basic Microbead (70ºC reaction)
    $740.00
    $925.00
    product image
    Buy now
    PrimeIAmp™ HotStart Bst DNA/RNA LHNB LyoMix (65ºC reaction)
    $660.00 - $2,000.00
    $2,500.00
    product image
    Buy now
    PrimeIAmp™ HotStart Bst DNA/RNA Basic & SYBR Green LyoMix (65ºC reaction)
    $660.00 - $2,000.00
    $2,500.00
    product image
    Buy now
    PrimeIAmp™ HotStart Bst DNA/RNA LHNB Microbead (65ºC reaction)
    $740.00
    $925.00
    product image
    Buy now
    PrimeIAmp™ HotStart Bst DNA/RNA Basic Microbead (65ºC reaction)
    $740.00
    $925.00

  • Customized Lyophilized Microbead Service 

    Look no further than our customized lyophilized microbead services! We offer lyophilized microbeads with customized primers and reaction volumes of 20-100 μl per bead, ensuring that your experiments are optimized to your exact specifications. Don't settle for a one-size-fits-all approach - Contact us today to learn more about how our customized lyophilized bead services can take your research to the next level!

  • Published Papers

    Representative Publications Using SBS Genetech Isothermal Amplification Products

     

    Xue, T. , Ma, Z. , Liu, F. , Du, W. , & An, C. . (2020). Pneumocystis jirovecii colonization and its association with pulmonary diseases: a multicenter study based on a modified loop-mediated isothermal amplification assay. BMC Pulmonary Medicine, 20(1).

     

    Zhao, K. , Hu, R. , Ni, J. , Liang, J. , & Li, C. . (2020). Establishment of a porcine parvovirus (PPV) LAMP visual rapid detection method. Journal of Virological Methods, 284, 113924.

     

    Papadakis, G. , Pantazis, A. K. , Fikas, N. , Chatziioannidou, S. , Tsiakalou, V. , & Michaelidou, K. , et al. (2022). Portable real-time colorimetric LAMP-device for rapid quantitative detection of nucleic acids in crude samples. Scientific Reports, 12(1), 1-15.

     

    Wu, R. , Liu, X. , Guo, B. , Chen, F. , & Wang, X. . (2014). Development of double loop-mediated isothermal amplification to detect listeria monocytogenes in food. Current Microbiology, 69(6), 839-845.

     

    Ji, J. , Du, L. Q. , Xie, Q. M. , Cao, Y. C. , & Bee, Y. Z. . (2009). Rapid diagnosis of duck plagues virus infection by loop-mediated isothermal amplification. Research in Veterinary Science, 87(1), 53-58.

     

    Zhang, S. , Xu, X. , Wu, Q. , & Zhang, J. . (2013). Rapid and sensitive detection of pseudomonas aeruginosain bottled water by loop-mediated isothermal amplification. European Food Research & Technology, 236(1), 209-215.

     

    Xu, X. , Zhang, S. , Wu, Q. , Zhang, J. , Li, F. , & Cheng, J. . (2014). Development and Application of a Loop-Mediated Isothermal Amplification (LAMP) Method for Rapid and Sensitive Detection of Enterococcus faecalis in Drinking Water. Journal of Food Safety, 34(2), 103–110.

     

    Zhang, F. , Shi, Y. , Jiang, K. , Song, W. , Ma, C. , & Xu, Z. , et al. (2014). Rapid detection and quantification of Prorocentrum minimum by loop-mediated isothermal amplification and real-time fluorescence quantitative PCR. Journal of Applied Phycology, 26(3), 1379-1388.

  • Cold Spring Harbor Laboratory - SBS Genetech

    SBS Genetech is a long-term sponsor of

    Cold Spring Harbor Laboratory

  • Contact

    For more information, please fill out the provided form or contact us directly by E-mail

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