PrimeIAmp™ HotStart LAMP Bst P L-HNB MasterMix
$400.00 - $1,600.00
$2,000.00
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: ILHPLL-100 (for 100T)
Cat. No.: ILHPLL-500 (for 400T)
Description
PrimeIAmp™ HotStart LAMP Bst P L-HNB MasterMix is a 4× semi‑premixed solution that can be directly used for lyophilization experiments without any additional auxiliary reagents. The premix contains reaction buffer, dNTPs, Mg²⁺, HotStart Bst P polymerase, trehalose, mannitol, and other stabilizers.
L-HNB dye in the product combines with Mg2+ to produce deep violet color before the reaction starts. With the LAMP reaction, the resulting PPi (inorganic pyrophosphate) combines with Mg2+ in the system, making L-HNB show light green.
(L-HNB-Mg2+ Compound) → (PPi-Mg2+) + (L-HNB)
Deep violet Light green
Features
Bst P DNA/RNA System has the following performance:
- The whole Bst P DNA/RNA System includes hot start Aptamer, which ensures that the enzyme activity blocking efficiency is >95% at <30°C, and the enzyme activity is completely released within 1 min at >60°C. This characteristic facilitates the establishment of the reaction system at room temperature and greatly reduces the non-specific amplification at low temperatures.
- The reaction temperature is further raised to 70°C, which greatly reduces the formation of primer dimer, improves the amplification specificity, and makes the nucleic acid release of crude samples more sufficient.
- The whole portfolio contains Helicase, so Premium LAMP amplification (pLAMP) is allowed without using F3/B3 primers. At the same time, Helicase has the function of assisting in strand unwinding, which further reduces the concentration of FIP/BIP primers. This will further reduce non-specific amplification and greatly improve amplification homogeneity.
Components
4 × Bst P Basic Mix | 500 μl |
50 × L-HNB Dye | 45 μl |
10 × GuHCl | 1.5 ml |
Storage
For long-term storage, keep the product at –20 °C or below (stable for up to 18 months). The reagent can withstand up to 10 freeze–thaw cycles without performance loss, though repeated freeze–thawing should be avoided when possible. For short-term use, storage at 2–8 °C is recommended, where the product remains stable for up to 2 weeks. Due to the high concentration of lyophilization excipients, a small amount of white insoluble material may be observed upon thawing; this will fully dissolve after 3–5 minutes at 30 °C (or by warming in the palm), without affecting product performance.
Storage
Store at –20 °C or below for up to 24 months. The product typically melts within 3–5 minutes at 28 °C, and up to 20 freeze–thaw cycles do not affect performance.
Instruction: Protocol
Viewpoint
Please find below the link of our viewpoint on the isothermal amplification industry, which is invited by APAC CIO Outlook, a digital and print magazine that identifies and profiles emerging companies providing cutting-edge solutions to enterprises in APAC.
SBS Genetech: Leading the Next Generation Isothermal Amplification Technology
SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data
Only for research and not intended for treatment of humans or animals
Journals Using SBS Genetech Products Universities Using SBS Genetech Products
SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory
