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Cat. No.: ICMB-5 (for 5 mL, 10%(v/v), 30-150 μm)

Cat. No.: ICMB-100 (for 2x50 mL, 10%(v/v), 30-150 μm)

Cat. No.: ICMB-1k (for 4x250 mL, 10%(v/v), 30-150 μm)

Description

IDA-Co Magnetic Agarose Beads for His-Tag Protein Purification possess superparamagnetic properties and are a novel functional material designed for the efficient and rapid purification of histidine-tagged proteins. These beads enable the direct one-step purification of high-purity target proteins from biological samples through magnetic separation, significantly simplifying the purification process and enhancing efficiency. They are ideal for the convenient purification of histidine-tagged proteins in both research and industrial applications.

Compared to traditional column chromatography methods using metal-chelated agarose prepacked columns, using magnetic beads for purifying histidine-tagged proteins eliminates the need for multiple rounds of lengthy high-speed centrifugation and membrane filtration. There is no need to control flow rates or use expensive chromatography equipment. The specific binding, washing, and elution of target proteins with the magnetic beads are straightforward, quick, and easy to perform. Skilled operators can obtain high-purity target proteins within one hour and easily handle high-throughput and large-scale parallel sample processing, saving time and costs for researchers.

Features

• Direct Purification from Crude Samples: Significantly shortens purification time.
• Easy Control of Protein Concentration and Volume: Adjusting elution buffer volume allows precise control over protein concentration and volume without protein loss, denaturation, or precipitation.
• High Purity in One-Step Purification: A complete procedure can yield target protein purity exceeding 95%.
• High Stability in Parallel Operations: Enables high-throughput and large-scale protein purification with a standard deviation of <5% in protein purity and yield. No restrictions on flow rate or sample volume.
• High Yield of Target Protein: After a complete purification process, the target protein with purity above 90% typically has a yield of 70-80%.
• Reusable with Simple Regeneration Process: The magnetic beads maintain excellent protein binding capacity and purity even after up to six regeneration cycles.
• Comparison of Nickel-Chelated and Cobalt-Chelated Magnetic Beads for His-tag Protein Purification:
  • Cobalt ion-chelated beads have slightly lower protein binding capacity than nickel ion-chelated beads but offer higher purity.
  • Cobalt ion-chelated beads require lower concentrations of imidazole for washing and elution compared to nickel ion-chelated beads.

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory