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Scarlet™ Blood Direct PCR Kit (EDTA)
$425.00 - $1,180.00
$1,835.00
Scarlet™ Blood Direct PCR Kit (EDTA) is a research use only kit, which is ideal for direct PCR identification of EDTA anticoagulant whole blood without DNA purification. The 2 × Scarlet™ Blood Direct PCR Mix (EDTA) provided by this kit has strong inhibitor tolerance. The maximum volume of human blood sample as PCR template can reach 45% of PCR system and 20% for mouse blood. Scarlet™ Blood Direct PCR Kit (EDTA) can sensitively amplify genomic and exogenous DNA fragments in blood samples.
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Features
- The whole blood is directly used as a PCR template, without time-consuming and expensive DNA purification and pretreatment.
- The system has strong amplification ability and can sensitively detect genomic and exogenous DNA fragments in the blood.
- The 2× Scarlet™ Blood Direct PCR Mix (EDTA) provided by this kit has strong inhibitor tolerance
- The sample is operated in a fully enclosed condition, preventing either sample contamination or false positive PCR results.
- The Scarlet™ Blood Direct PCR Kit (EDTA) is specially designed for direct PCR identification of EDTA anticoagulant whole blood.
Limitations
- Amplified fragments should be less than 1 kb. If more than 1 kb, the amplification efficiency may decrease or the amplification may even fail.
- If PCR products are used for sequencing, we recommend purifying the PCR products first.
- PCR products may have mutation.
- The 3' end of the PCR product is randomly added with A tail.
Content
500T
2,000T
Storage
If used frequently, 2 × Scarlet™ Blood Direct PCR Mix (EDTA) can be stored at 4°C for a short time (within 10 days). For long term storage, please keep it at - 20°C.
6 × DNA Loading Buffer can be stored at 4°C or - 20°C for a long time.
Instruction: Protocol