Adding Carrier RNA to the column purified nucleic acid purification system can improve the recovery efficiency of more than 10 times of the trace nucleic acid. At the same time, due to the presence of Carrier RNA, it can also protect the trace RNA template, and reduce the damage caused by the RNase on the trace RNA template. Carrier RNA is an indispensable component of column-based microRNA purification.
Add 1-5 μL Carrier RNA to the lysis buffer for each purification system. The final extracted nucleic acid containing Carrier RNA can be directly used in PCR or RT-PCR reactions.