TransXG™ Transfection Reagent has high transfection efficiency, good repeatability, and simple operation for most adherent cells. Transfection reagents and plasmid DNA can form nanoparticles within 5 min at room temperature. The formed DNA nanocomplexes can be directly added into the complete cell culture medium without the need to remove the transfection reagents and DNA complexes by replacing the culture medium to reduce the cytotoxicity of the transfection agent, nor the need to use specific transfection-specific media such as MEM. The control experiment showed that the transfection effect of this product was not affected by serum and antibiotics in the culture medium, and the best transfection efficiency could be obtained by simply adjusting the ratio of transfection reagent and plasmid DNA. This reagent has obtained ideal results in the transfection experiments of HEK293, HeLa, MCF-7, COS-7, CHO, HepG2, NIH-3T3, L929 and 4T1 cells.
Stored at 2-8℃ for one year and -20℃ for two years.