Cat. No.: T4DL
5 × T4 DNA Ligase Buffer
250 mMTris-HCl (pH 7.5), 50mM MgCl2, 50mM DTT, 5mM ATP, Connection promoter.
Purified from E. coli strain carrying T4 DNA ligase gene.
NaCl or KCl with more than 200 mM can strongly inhibit ligase activity. It can be inactivated by heating at 65℃ for 10 min or 70℃ for 5 min.
In T4 DNA ligase reaction buffer, the amount of enzyme required to ligate 50% of Hind III digested lambda DNA fragments at 16℃ for 30 min is a unit.
Blue-white spotting test shows that the number of white spots is less than 2%. Excess T4 DNA Ligase mixed superhelical plasmid test shows no nuclease detection. SDS-PAGE test of recombinant protein purity is more than 99%.