Cat. No.: SP-250
sPfu (super Pfu) DNA polymerase catalyzes the polymerization of nucleotides into duplex DNA in the 5’→3’ direction, resulting in blunt-ended PCR products without 3’-dA overhangs. sPfu DNA Polymerase exhibits 3’→5’ exonuclease (proofreading) activity that enables the polymerase to correct mis-incorporated nucleotides, and lacks 5’→3’ exonuclease activity. It is suitable for PCR and primer extension reaction that requires high fidelity when the PCR fragment is relatively shorter. The fidelity is 64 times higher than Taq DNA Polymerase, and 8 times higher than regular Pfu DNA Polymerase.
Instruction: sPfu DNA Polymerase
Only for research and not intended for treatment of humans or animals