SiMax™ Plasmid DNA Miniprep (50 preps)
The SiMax™ Plasmid DNA Miniprep Kit is designed for the rapid purification of plasmid DNA. In a high-salt buffer, DNA is bound to the SiMax™membrane in a spin column. Following a wash step, the DNA is eluted in low-salt buffer or water without alcohol precipitation or desalting. 5~15 μg of a high-copy number plasmid DNA can be isolated from 3 ml of bacterial culture in 30 minutes. This kit removes proteins, RNA, and low molecular weight impurities. The high-quality plasmid DNA can be used for restriction enzyme digestion, PCR, automated sequencing, manual sequencing, and transformation.